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Article: Mammalian cells use the autophagy process to restrict avian influenza virus replication

TitleMammalian cells use the autophagy process to restrict avian influenza virus replication
Authors
Keywordsinfluenza
PB2
adaptationautophagy
cross species transmission
avian influenza
Issue Date2021
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports
Citation
Cell Reports, 2021, v. 35 n. 10, p. article no. 109213 How to Cite?
AbstractHost adaptive mutations in the influenza A virus (IAV) PB2 protein are critical for human infection, but their molecular action is not well understood. We observe that when IAV containing avian PB2 infects mammalian cells, viral ribonucleoprotein (vRNP) aggregates that localize to the microtubule-organizing center (MTOC) are formed. These vRNP aggregates resemble LC3B-associated autophagosome structures, with aggresome-like properties, in that they cause the re-distribution of vimentin. However, electron microscopy reveals that these aggregates represent an accumulation of autophagic vacuoles. Compared to mammalian-PB2 virus, avian-PB2 virus induces higher autophagic flux in infected cells, indicating an increased rate of autophagosomes containing avian vRNPs fusing with lysosomes. We found that p62 is essential for the formation of vRNP aggregates and that the Raptor-interacting region of p62 is required for interaction with vRNPs through the PB2 polymerase subunit. Selective autophagic sequestration during late-stage virus replication is thus an additional strategy for host restriction of avian-PB2 IAV.
Persistent Identifierhttp://hdl.handle.net/10722/305001
ISSN
2021 Impact Factor: 9.995
2020 SCImago Journal Rankings: 6.264
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, S-
dc.contributor.authorMok, BWY-
dc.contributor.authorDeng, S-
dc.contributor.authorLiu, H-
dc.contributor.authorWang, P-
dc.contributor.authorSong, W-
dc.contributor.authorChen, P-
dc.contributor.authorHuang, X-
dc.contributor.authorZheng, M-
dc.contributor.authorLau, SY-
dc.contributor.authorCremin, CJ-
dc.contributor.authorTam, CY-
dc.contributor.authorLi, B-
dc.contributor.authorJiang, L-
dc.contributor.authorChen, Y-
dc.contributor.authorYuen, KY-
dc.contributor.authorChen, H-
dc.date.accessioned2021-10-05T02:38:19Z-
dc.date.available2021-10-05T02:38:19Z-
dc.date.issued2021-
dc.identifier.citationCell Reports, 2021, v. 35 n. 10, p. article no. 109213-
dc.identifier.issn2211-1247-
dc.identifier.urihttp://hdl.handle.net/10722/305001-
dc.description.abstractHost adaptive mutations in the influenza A virus (IAV) PB2 protein are critical for human infection, but their molecular action is not well understood. We observe that when IAV containing avian PB2 infects mammalian cells, viral ribonucleoprotein (vRNP) aggregates that localize to the microtubule-organizing center (MTOC) are formed. These vRNP aggregates resemble LC3B-associated autophagosome structures, with aggresome-like properties, in that they cause the re-distribution of vimentin. However, electron microscopy reveals that these aggregates represent an accumulation of autophagic vacuoles. Compared to mammalian-PB2 virus, avian-PB2 virus induces higher autophagic flux in infected cells, indicating an increased rate of autophagosomes containing avian vRNPs fusing with lysosomes. We found that p62 is essential for the formation of vRNP aggregates and that the Raptor-interacting region of p62 is required for interaction with vRNPs through the PB2 polymerase subunit. Selective autophagic sequestration during late-stage virus replication is thus an additional strategy for host restriction of avian-PB2 IAV.-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports-
dc.relation.ispartofCell Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectinfluenza-
dc.subjectPB2-
dc.subjectadaptationautophagy-
dc.subjectcross species transmission-
dc.subjectavian influenza-
dc.titleMammalian cells use the autophagy process to restrict avian influenza virus replication-
dc.typeArticle-
dc.identifier.emailLiu, S: siwenliu@hku.hk-
dc.identifier.emailMok, BWY: bobomok@hku.hk-
dc.identifier.emailWang, P: puiwang@hkucc.hku.hk-
dc.identifier.emailSong, W: wjsong@hkucc.hku.hk-
dc.identifier.emailTam, CY: rach2011@hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.emailChen, H: hlchen@hku.hk-
dc.identifier.authorityYuen, KY=rp00366-
dc.identifier.authorityChen, H=rp00383-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.celrep.2021.109213-
dc.identifier.pmid34107256-
dc.identifier.scopuseid_2-s2.0-85107441987-
dc.identifier.hkuros326074-
dc.identifier.volume35-
dc.identifier.issue10-
dc.identifier.spagearticle no. 109213-
dc.identifier.epagearticle no. 109213-
dc.identifier.isiWOS:000659894300006-
dc.publisher.placeUnited States-

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