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- Publisher Website: 10.1016/j.jdent.2020.103528
- Scopus: eid_2-s2.0-85097739620
- PMID: 33188848
- WOS: WOS:000619736100019
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Article: Enamel remineralization potential of arginine-fluoride varnish in a multi-species bacterial pH-cycling model
Title | Enamel remineralization potential of arginine-fluoride varnish in a multi-species bacterial pH-cycling model |
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Authors | |
Keywords | Arginine Caries Enamel Fluoride Varnish |
Issue Date | 2021 |
Publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent |
Citation | Journal of Dentistry, 2021, v. 104, p. article no. 103528 How to Cite? |
Abstract | Objectives:
To examine the enamel fluoride uptake and remineralization potential of arginine-fluoride (Arg-NaF) varnishes in a simulated clinical condition using a multi-species bacterial pH-cycling model.
Methods:
L-Arginine (at 1 %, 2 %, and 4 % by wt.) was incorporated in a 5 % NaF varnish. Experimental and control groups were: 1 % Arg-NaF; 2 % Arg-NaF; 4 % Arg-NaF; NaF and no treatment. Artificial incipient caries-like lesions were formed on 30 enamel specimen blocks (n = 6). The specimens underwent multi-species bacterial pH-cycling in an artificial mouth system using oral biofilm reactor for 72 h after treatment. The specimens were evaluated for mineral density using micro-CT, Ca/P ratio with SEM-EDX, enamel fluoride uptake (EFU) and plaque fluoride uptake (PFU).
Results:
Increasing concentrations of Arg in NaF varnish significantly increased the EFU of incipient caries-like lesions (p < 0.001). The PFU for 1 % Arg-NaF was significantly higher than 4 % Arg-NaF and the control NaF (p < 0.05). Post pH-cycling, Ca/P ratio with 1 %/2 % Arg-NaF was closest to hydroxyapatite (1.67). Mineral gain and % remineralization of 1 %/2 % Arg-NaF was significantly higher than the control NaF varnish (p < 0.05).
Conclusion:
The prebiotic L-arginine (at 1 %/2 % by wt.) in a 5 % NaF varnish enhanced the enamel fluoride uptake and remineralization potential of the conventional 5 % NaF varnish.
Clinical significance:
The Arg-NaF varnish addresses the limitations of fluorides on cariogenic biofilms. The Arg-NaF varnish appears a promising caries-preventive regimen that counters the pathogenic biofilms by Arg and promotes remineralization with fluorides. In high caries-risk patients, professional application of Arg-NaF varnish might aid to alleviate the global burden of dental caries. |
Persistent Identifier | http://hdl.handle.net/10722/305261 |
ISSN | 2021 Impact Factor: 4.991 2020 SCImago Journal Rankings: 1.504 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | BIJLE, MN | - |
dc.contributor.author | ABDALLA, MM | - |
dc.contributor.author | ASHRAF, U | - |
dc.contributor.author | Ekambaram, M | - |
dc.contributor.author | Yiu, CKY | - |
dc.date.accessioned | 2021-10-20T10:06:55Z | - |
dc.date.available | 2021-10-20T10:06:55Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Journal of Dentistry, 2021, v. 104, p. article no. 103528 | - |
dc.identifier.issn | 0300-5712 | - |
dc.identifier.uri | http://hdl.handle.net/10722/305261 | - |
dc.description.abstract | Objectives: To examine the enamel fluoride uptake and remineralization potential of arginine-fluoride (Arg-NaF) varnishes in a simulated clinical condition using a multi-species bacterial pH-cycling model. Methods: L-Arginine (at 1 %, 2 %, and 4 % by wt.) was incorporated in a 5 % NaF varnish. Experimental and control groups were: 1 % Arg-NaF; 2 % Arg-NaF; 4 % Arg-NaF; NaF and no treatment. Artificial incipient caries-like lesions were formed on 30 enamel specimen blocks (n = 6). The specimens underwent multi-species bacterial pH-cycling in an artificial mouth system using oral biofilm reactor for 72 h after treatment. The specimens were evaluated for mineral density using micro-CT, Ca/P ratio with SEM-EDX, enamel fluoride uptake (EFU) and plaque fluoride uptake (PFU). Results: Increasing concentrations of Arg in NaF varnish significantly increased the EFU of incipient caries-like lesions (p < 0.001). The PFU for 1 % Arg-NaF was significantly higher than 4 % Arg-NaF and the control NaF (p < 0.05). Post pH-cycling, Ca/P ratio with 1 %/2 % Arg-NaF was closest to hydroxyapatite (1.67). Mineral gain and % remineralization of 1 %/2 % Arg-NaF was significantly higher than the control NaF varnish (p < 0.05). Conclusion: The prebiotic L-arginine (at 1 %/2 % by wt.) in a 5 % NaF varnish enhanced the enamel fluoride uptake and remineralization potential of the conventional 5 % NaF varnish. Clinical significance: The Arg-NaF varnish addresses the limitations of fluorides on cariogenic biofilms. The Arg-NaF varnish appears a promising caries-preventive regimen that counters the pathogenic biofilms by Arg and promotes remineralization with fluorides. In high caries-risk patients, professional application of Arg-NaF varnish might aid to alleviate the global burden of dental caries. | - |
dc.language | eng | - |
dc.publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent | - |
dc.relation.ispartof | Journal of Dentistry | - |
dc.subject | Arginine | - |
dc.subject | Caries | - |
dc.subject | Enamel | - |
dc.subject | Fluoride | - |
dc.subject | Varnish | - |
dc.title | Enamel remineralization potential of arginine-fluoride varnish in a multi-species bacterial pH-cycling model | - |
dc.type | Article | - |
dc.identifier.email | Yiu, CKY: ckyyiu@hkucc.hku.hk | - |
dc.identifier.authority | Ekambaram, M=rp02026 | - |
dc.identifier.authority | Yiu, CKY=rp00018 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jdent.2020.103528 | - |
dc.identifier.pmid | 33188848 | - |
dc.identifier.scopus | eid_2-s2.0-85097739620 | - |
dc.identifier.hkuros | 328325 | - |
dc.identifier.volume | 104 | - |
dc.identifier.spage | article no. 103528 | - |
dc.identifier.epage | article no. 103528 | - |
dc.identifier.isi | WOS:000619736100019 | - |
dc.publisher.place | United Kingdom | - |