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Article: Rationally designed protein cross-linked hydrogel for bone regeneration via synergistic release of magnesium and zinc ions

TitleRationally designed protein cross-linked hydrogel for bone regeneration via synergistic release of magnesium and zinc ions
Authors
KeywordsProtein engineering
Protein crosslinker
Smart delivery system
Synergistic effect of bioactive ions
Bone-tissue engineering
Issue Date2021
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2021, v. 274, p. article no. 120895 How to Cite?
AbstractThe development of recombinant protein cross-linked injectable hydrogels with good mechanical strength and effective drug loading capacity for bone regeneration is extremely attractive and rarely reported. Here, we report the fabrication of a smart hydrogel delivery system by incorporating a rationally designed T4 lysozyme mutant (T4M) to mediate the localized delivery and synergistic release of Mg2+ and Zn2+ for bone repair. Apart from its intrinsic antibacterial properties, T4M bears abundant free amine groups on its surface to function as effective covalent crosslinkers to strengthen the hydrogel network as well as exhibits specific binding affinity to multivalent cations such as Zn2+. Moreover, the integrin receptor-binding Arg-Gly-Asp (RGD) sequence was introduced onto the C-terminus of T4 lysozyme to improve its cellular affinity and further facilitate rapid tissue regeneration. The final composite hydrogel displays excellent injectability, improved mechanical properties, antibacterial activity, and unique bioactivities. The effective loading of Mg2+/Zn2+ in the hydrogels could mediate the sequential and sustained release of Mg2+ and Zn2+, thereby resulting in synergistic enhancement on bone regeneration through modulation of the MAPK signaling pathway. We believe that the strategy proposed in this paper opens up a new route for developing protein cross-linked smart delivery systems for tissue regeneration.
DescriptionHybrid open access
Persistent Identifierhttp://hdl.handle.net/10722/305408
ISSN
2023 Impact Factor: 12.8
2023 SCImago Journal Rankings: 3.016
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, X-
dc.contributor.authorTan, B-
dc.contributor.authorWang, S-
dc.contributor.authorTang, R-
dc.contributor.authorBao, Z-
dc.contributor.authorChen, G-
dc.contributor.authorChen, S-
dc.contributor.authorTang, W-
dc.contributor.authorWang, Z-
dc.contributor.authorLong, C-
dc.contributor.authorLu, WW-
dc.contributor.authorYang, D-
dc.contributor.authorBian, L-
dc.contributor.authorPeng, S-
dc.date.accessioned2021-10-20T10:08:59Z-
dc.date.available2021-10-20T10:08:59Z-
dc.date.issued2021-
dc.identifier.citationBiomaterials, 2021, v. 274, p. article no. 120895-
dc.identifier.issn0142-9612-
dc.identifier.urihttp://hdl.handle.net/10722/305408-
dc.descriptionHybrid open access-
dc.description.abstractThe development of recombinant protein cross-linked injectable hydrogels with good mechanical strength and effective drug loading capacity for bone regeneration is extremely attractive and rarely reported. Here, we report the fabrication of a smart hydrogel delivery system by incorporating a rationally designed T4 lysozyme mutant (T4M) to mediate the localized delivery and synergistic release of Mg2+ and Zn2+ for bone repair. Apart from its intrinsic antibacterial properties, T4M bears abundant free amine groups on its surface to function as effective covalent crosslinkers to strengthen the hydrogel network as well as exhibits specific binding affinity to multivalent cations such as Zn2+. Moreover, the integrin receptor-binding Arg-Gly-Asp (RGD) sequence was introduced onto the C-terminus of T4 lysozyme to improve its cellular affinity and further facilitate rapid tissue regeneration. The final composite hydrogel displays excellent injectability, improved mechanical properties, antibacterial activity, and unique bioactivities. The effective loading of Mg2+/Zn2+ in the hydrogels could mediate the sequential and sustained release of Mg2+ and Zn2+, thereby resulting in synergistic enhancement on bone regeneration through modulation of the MAPK signaling pathway. We believe that the strategy proposed in this paper opens up a new route for developing protein cross-linked smart delivery systems for tissue regeneration.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials-
dc.relation.ispartofBiomaterials-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectProtein engineering-
dc.subjectProtein crosslinker-
dc.subjectSmart delivery system-
dc.subjectSynergistic effect of bioactive ions-
dc.subjectBone-tissue engineering-
dc.titleRationally designed protein cross-linked hydrogel for bone regeneration via synergistic release of magnesium and zinc ions-
dc.typeArticle-
dc.identifier.emailLu, WW: wwlu@hku.hk-
dc.identifier.authorityLu, WW=rp00411-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.biomaterials.2021.120895-
dc.identifier.pmid34020269-
dc.identifier.scopuseid_2-s2.0-85106306759-
dc.identifier.hkuros327920-
dc.identifier.volume274-
dc.identifier.spagearticle no. 120895-
dc.identifier.epagearticle no. 120895-
dc.identifier.isiWOS:000663586500001-
dc.publisher.placeNetherlands-

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