File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants

TitleTropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants
Authors
KeywordsCOVID-19
SARS-CoV-2
SARS-CoV
Influenza
Dogs
Ex vivo
Explants
Issue Date2021
PublisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
Citation
The Journal of Infectious Diseases, 2021, v. 224 n. 5, p. 821-830 How to Cite?
AbstractBackground: Human spillovers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to dogs and the emergence of a highly contagious avian-origin H3N2 canine influenza virus have raised concerns on the role of dogs in the spread of SARS-CoV-2 and their susceptibility to existing human and avian influenza viruses, which might result in further reassortment. Methods: We systematically studied the replication kinetics of SARS-CoV-2, SARS-CoV, influenza A viruses of H1, H3, H5, H7, and H9 subtypes, and influenza B viruses of Yamagata-like and Victoria-like lineages in ex vivo canine nasal cavity, soft palate, trachea, and lung tissue explant cultures and examined ACE2 and sialic acid (SA) receptor distribution in these tissues. Results: There was limited productive replication of SARS-CoV-2 in canine nasal cavity and SARS-CoV in canine nasal cavity, soft palate, and lung, with unexpectedly high ACE2 levels in canine nasal cavity and soft palate. Canine tissues were susceptible to a wide range of human and avian influenza viruses, which matched with the abundance of both human and avian SA receptors. Conclusions: Existence of suitable receptors and tropism for the same tissue foster virus adaptation and reassortment. Continuous surveillance in dog populations should be conducted given the many chances for spillover during outbreaks.
Persistent Identifierhttp://hdl.handle.net/10722/306103
ISSN
2020 Impact Factor: 5.226
2020 SCImago Journal Rankings: 2.690
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBui, CHT-
dc.contributor.authorYeung, HW-
dc.contributor.authorHo, JCW-
dc.contributor.authorLeung, CYH-
dc.contributor.authorHui, KPY-
dc.contributor.authorPerera, RAPM-
dc.contributor.authorWebby, RJ-
dc.contributor.authorSchultz-Cherry, SL-
dc.contributor.authorNicholls, JM-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorChan, MCW-
dc.date.accessioned2021-10-20T10:18:50Z-
dc.date.available2021-10-20T10:18:50Z-
dc.date.issued2021-
dc.identifier.citationThe Journal of Infectious Diseases, 2021, v. 224 n. 5, p. 821-830-
dc.identifier.issn0022-1899-
dc.identifier.urihttp://hdl.handle.net/10722/306103-
dc.description.abstractBackground: Human spillovers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to dogs and the emergence of a highly contagious avian-origin H3N2 canine influenza virus have raised concerns on the role of dogs in the spread of SARS-CoV-2 and their susceptibility to existing human and avian influenza viruses, which might result in further reassortment. Methods: We systematically studied the replication kinetics of SARS-CoV-2, SARS-CoV, influenza A viruses of H1, H3, H5, H7, and H9 subtypes, and influenza B viruses of Yamagata-like and Victoria-like lineages in ex vivo canine nasal cavity, soft palate, trachea, and lung tissue explant cultures and examined ACE2 and sialic acid (SA) receptor distribution in these tissues. Results: There was limited productive replication of SARS-CoV-2 in canine nasal cavity and SARS-CoV in canine nasal cavity, soft palate, and lung, with unexpectedly high ACE2 levels in canine nasal cavity and soft palate. Canine tissues were susceptible to a wide range of human and avian influenza viruses, which matched with the abundance of both human and avian SA receptors. Conclusions: Existence of suitable receptors and tropism for the same tissue foster virus adaptation and reassortment. Continuous surveillance in dog populations should be conducted given the many chances for spillover during outbreaks.-
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org-
dc.relation.ispartofThe Journal of Infectious Diseases-
dc.subjectCOVID-19-
dc.subjectSARS-CoV-2-
dc.subjectSARS-CoV-
dc.subjectInfluenza-
dc.subjectDogs-
dc.subjectEx vivo-
dc.subjectExplants-
dc.titleTropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants-
dc.typeArticle-
dc.identifier.emailBui, CHT: clyyee@hku.hk-
dc.identifier.emailYeung, HW: hinwo@hku.hk-
dc.identifier.emailHo, JCW: cwjohn@connect.hku.hk-
dc.identifier.emailLeung, CYH: cyhleung@hku.hk-
dc.identifier.emailHui, KPY: kenrie@hku.hk-
dc.identifier.emailNicholls, JM: jmnichol@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.emailChan, MCW: mchan@hku.hk-
dc.identifier.authorityLeung, CYH=rp00307-
dc.identifier.authorityHui, KPY=rp02149-
dc.identifier.authorityPerera, RAPM=rp02500-
dc.identifier.authorityNicholls, JM=rp00364-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.identifier.authorityChan, MCW=rp00420-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/infdis/jiab002-
dc.identifier.pmid33395484-
dc.identifier.pmcidPMC7799041-
dc.identifier.scopuseid_2-s2.0-85116957339-
dc.identifier.hkuros327840-
dc.identifier.volume224-
dc.identifier.issue5-
dc.identifier.spage821-
dc.identifier.epage830-
dc.identifier.isiWOS:000703933800013-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats