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Article: Lingzhi and San-Miao-San with hyaluronic acid gel mitigate cartilage degeneration in anterior cruciate ligament transection induced osteoarthritis

TitleLingzhi and San-Miao-San with hyaluronic acid gel mitigate cartilage degeneration in anterior cruciate ligament transection induced osteoarthritis
Authors
KeywordsArticular cartilage
Hyaluronic acid gel
Lingzhi and San-Miao-San
Osteoarthritis
Subchondral trabecular bone
Issue Date2021
PublisherElsevier for the Chinese Speaking Orthopaedic Society and the International Chinese Musculoskeletal Research Society. The Journal's web site is located at http://www.elsevier.com/journals/journal-of-orthopaedic-translation/2214-031x#
Citation
Journal of Orthopaedic Translation, 2021, v. 26, p. 132-140 How to Cite?
AbstractObjective: To investigate the mitigate efficacy of Chinese medicine Lingzhi (LZ) and San-Miao-San (SMS) combined with hyaluronic acid (HA)-gel in attenuating cartilage degeneration in traumatic osteoarthritis (OA). Methods: The standardized surgery of anterior cruciate ligament transection (ACLT) was made from the medial compartment of right hind limbs of 8-week-old female SD rats and resulted in a traumatic OA. Rats (n ​= ​5/group) were treated once intra-articular injection of 50 ​μl HA-gel, 50 ​μl HA-gel+50 ​μg LZ-SMS, 50 ​μl of saline+50 ​μg LZ-SMS and null (ACLT group) respectively, except sham group. Limbs were harvested for μCT scan and histopathological staining 3-month post-treatment. Inflammatory cytokines from plasma and synovial fluid were detected using Immunology Multiplex Assay kit. The putative targets of active compounds in LZ-SMS and known therapeutic targets for OA were combined to construct protein–protein interaction network. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was adopted to predict the potential targets and signaling pathway of LZ-SMS in OA through the tool of DAVID Bioinformatics. Results: In vivo, HA-gel ​+ ​LZ-SMS treatment resulted in a higher volume ratio of hyaline cartilage (HC)/calcified cartilage (CC) and HC/Sum (total volume of cartilage), compared to ACLT and HA-gel groups. In addition, histological results showed the elevated cartilage matrix, chondrogenic and osteoblastic signals in HA-gel ​+ ​LZ-SMS treatment. Treatment also significantly altered subchondral bone (SCB) structure including an increase in BV/TV, Tb.Th, BMD, Conn.Dn, Tb.N, and DA, as well as a significant decrease in Tb.Sp and Po(tot), which implied a protective effect on maintaining the stabilization of tibial SCB microstructure. Furthermore, there was also a down-regulated inflammatory cytokines and upregulated anti-inflammatory cytokine IL-10 in HA+LZ-SMS group. Finally, 64 shared targets from 37 active compounds in LZ-SMS related to the core genes for the development of OA. LZ-SMS has a putative role in regulating inflammatory circumstance through influencing the MAPK signaling pathway. Conclusion: Our study elucidated a protective effect of HA-gel ​+ ​LZ-SMS in mitigating cartilage degradation and putative interaction with targets and signaling pathway for the development of traumatic OA. The translational potential of this article: Our results provide a biological rationale for the use of LZ-SMS as a potential candidate for OA treatment.
Persistent Identifierhttp://hdl.handle.net/10722/306163
ISSN
2023 Impact Factor: 5.9
2023 SCImago Journal Rankings: 1.259
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChu, M-
dc.contributor.authorWu, P-
dc.contributor.authorHong, M-
dc.contributor.authorZeng, H-
dc.contributor.authorWong, CK-
dc.contributor.authorFeng, Y-
dc.contributor.authorCai, Z-
dc.contributor.authorLu, WW-
dc.date.accessioned2021-10-20T10:19:41Z-
dc.date.available2021-10-20T10:19:41Z-
dc.date.issued2021-
dc.identifier.citationJournal of Orthopaedic Translation, 2021, v. 26, p. 132-140-
dc.identifier.issn2214-031X-
dc.identifier.urihttp://hdl.handle.net/10722/306163-
dc.description.abstractObjective: To investigate the mitigate efficacy of Chinese medicine Lingzhi (LZ) and San-Miao-San (SMS) combined with hyaluronic acid (HA)-gel in attenuating cartilage degeneration in traumatic osteoarthritis (OA). Methods: The standardized surgery of anterior cruciate ligament transection (ACLT) was made from the medial compartment of right hind limbs of 8-week-old female SD rats and resulted in a traumatic OA. Rats (n ​= ​5/group) were treated once intra-articular injection of 50 ​μl HA-gel, 50 ​μl HA-gel+50 ​μg LZ-SMS, 50 ​μl of saline+50 ​μg LZ-SMS and null (ACLT group) respectively, except sham group. Limbs were harvested for μCT scan and histopathological staining 3-month post-treatment. Inflammatory cytokines from plasma and synovial fluid were detected using Immunology Multiplex Assay kit. The putative targets of active compounds in LZ-SMS and known therapeutic targets for OA were combined to construct protein–protein interaction network. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was adopted to predict the potential targets and signaling pathway of LZ-SMS in OA through the tool of DAVID Bioinformatics. Results: In vivo, HA-gel ​+ ​LZ-SMS treatment resulted in a higher volume ratio of hyaline cartilage (HC)/calcified cartilage (CC) and HC/Sum (total volume of cartilage), compared to ACLT and HA-gel groups. In addition, histological results showed the elevated cartilage matrix, chondrogenic and osteoblastic signals in HA-gel ​+ ​LZ-SMS treatment. Treatment also significantly altered subchondral bone (SCB) structure including an increase in BV/TV, Tb.Th, BMD, Conn.Dn, Tb.N, and DA, as well as a significant decrease in Tb.Sp and Po(tot), which implied a protective effect on maintaining the stabilization of tibial SCB microstructure. Furthermore, there was also a down-regulated inflammatory cytokines and upregulated anti-inflammatory cytokine IL-10 in HA+LZ-SMS group. Finally, 64 shared targets from 37 active compounds in LZ-SMS related to the core genes for the development of OA. LZ-SMS has a putative role in regulating inflammatory circumstance through influencing the MAPK signaling pathway. Conclusion: Our study elucidated a protective effect of HA-gel ​+ ​LZ-SMS in mitigating cartilage degradation and putative interaction with targets and signaling pathway for the development of traumatic OA. The translational potential of this article: Our results provide a biological rationale for the use of LZ-SMS as a potential candidate for OA treatment.-
dc.languageeng-
dc.publisherElsevier for the Chinese Speaking Orthopaedic Society and the International Chinese Musculoskeletal Research Society. The Journal's web site is located at http://www.elsevier.com/journals/journal-of-orthopaedic-translation/2214-031x#-
dc.relation.ispartofJournal of Orthopaedic Translation-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectArticular cartilage-
dc.subjectHyaluronic acid gel-
dc.subjectLingzhi and San-Miao-San-
dc.subjectOsteoarthritis-
dc.subjectSubchondral trabecular bone-
dc.titleLingzhi and San-Miao-San with hyaluronic acid gel mitigate cartilage degeneration in anterior cruciate ligament transection induced osteoarthritis-
dc.typeArticle-
dc.identifier.emailLu, WW: wwlu@hku.hk-
dc.identifier.authorityLu, WW=rp00411-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.jot.2020.07.008-
dc.identifier.pmid33437632-
dc.identifier.pmcidPMC7773973-
dc.identifier.scopuseid_2-s2.0-85093652853-
dc.identifier.hkuros327521-
dc.identifier.volume26-
dc.identifier.spage132-
dc.identifier.epage140-
dc.identifier.isiWOS:000604132600016-
dc.publisher.placeNetherlands-

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