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Book Chapter: Synchronization of HeLa Cells

TitleSynchronization of HeLa Cells
Authors
KeywordsFlow cytometry
Cyclin
Synchronization
Cell cycle
Cyclin-dependent kinases
Issue Date2017
PublisherHumana Press.
Citation
Synchronization of HeLa Cells. In Banfalvi, G (Ed.), Cell Cycle Synchronization: Methods and Protocols (Second Edition), p. 189-201. New York, NY: Humana Press, 2017 How to Cite?
AbstractHeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studying the cell cycle. Here, we describe several protocols for synchronizing HeLa cells from different phases of the cell cycle, including G1 phase using the HMG-CoA reductase inhibitor lovastatin, S phase with a double thymidine block procedure, and G2 phase with the CDK1 inhibitor RO-3306. Cells can also be enriched in mitosis using nocodazole and mechanical shake-off. Releasing the cells from these blocks enables researchers to follow gene expression and other events through the cell cycle. We also describe several protocols, including flow cytometry, BrdU labeling, immunoblotting, and time-lapse microscopy, for validating the synchrony of the cells and monitoring the progression of the cell cycle.
Persistent Identifierhttp://hdl.handle.net/10722/307499
ISBN
ISSN
2023 SCImago Journal Rankings: 0.399
Series/Report no.Methods in Molecular Biology ; 1524

 

DC FieldValueLanguage
dc.contributor.authorMa, Hoi Tang-
dc.contributor.authorPoon, Randy Y.C.-
dc.date.accessioned2021-11-03T06:22:43Z-
dc.date.available2021-11-03T06:22:43Z-
dc.date.issued2017-
dc.identifier.citationSynchronization of HeLa Cells. In Banfalvi, G (Ed.), Cell Cycle Synchronization: Methods and Protocols (Second Edition), p. 189-201. New York, NY: Humana Press, 2017-
dc.identifier.isbn9781493966028-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/307499-
dc.description.abstractHeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studying the cell cycle. Here, we describe several protocols for synchronizing HeLa cells from different phases of the cell cycle, including G1 phase using the HMG-CoA reductase inhibitor lovastatin, S phase with a double thymidine block procedure, and G2 phase with the CDK1 inhibitor RO-3306. Cells can also be enriched in mitosis using nocodazole and mechanical shake-off. Releasing the cells from these blocks enables researchers to follow gene expression and other events through the cell cycle. We also describe several protocols, including flow cytometry, BrdU labeling, immunoblotting, and time-lapse microscopy, for validating the synchrony of the cells and monitoring the progression of the cell cycle.-
dc.languageeng-
dc.publisherHumana Press.-
dc.relation.ispartofCell Cycle Synchronization: Methods and Protocols (Second Edition)-
dc.relation.ispartofseriesMethods in Molecular Biology ; 1524-
dc.subjectFlow cytometry-
dc.subjectCyclin-
dc.subjectSynchronization-
dc.subjectCell cycle-
dc.subjectCyclin-dependent kinases-
dc.titleSynchronization of HeLa Cells-
dc.typeBook_Chapter-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-4939-6603-5_12-
dc.identifier.pmid27815904-
dc.identifier.scopuseid_2-s2.0-84994496483-
dc.identifier.spage189-
dc.identifier.epage201-
dc.publisher.placeNew York, NY-

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