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Article: Generation of GADD45A gene knockout human embryonic stem cell line using CRISPR/Cas9

TitleGeneration of GADD45A gene knockout human embryonic stem cell line using CRISPR/Cas9
Authors
Li, JLi, YZhan, XYRan, XTse, HFDang, SNie, YHuang, K
Issue Date2020
PublisherElsevier BV. The Journal's web site is located at https://journals.elsevier.com/stem-cell-research
Citation
Stem Cell Research, 2020, v. 49, article no. 102090 How to Cite?
DOI: http://dx.doi.org/10.1016/j.scr.2020.102090
AbstractGADD45A is a DNA damage and stressful growth arrest inducible protein, also it is shown to a be tumor suppressor gene and a chromatin relaxer associated with opening chromatin during the somatic reprogramming. However, its role in human embryonic stem cells and human embryonic stem cell modeled development has been merely documented. To illustrate the function of GADD45A in the human embryonic stem cell biology, we reported a GADD45A knockout human embryonic stem cell line by CRISPR/Cas9 mediated gene targeting. This cell line displayed normal karyotype, pluripotent stem cell marker expression and differentiation potential both in vivo and vitro.
Persistent Identifierhttp://hdl.handle.net/10722/307617
ISSN
1873-5061
2021 Impact Factor: 1.587
2020 SCImago Journal Rankings: 0.654
ISI Accession Number ID
WOS:000600952800089

 

DC FieldValueLanguage
dc.contributor.authorLi, J-
dc.contributor.authorLi, Y-
dc.contributor.authorZhan, XY-
dc.contributor.authorRan, X-
dc.contributor.authorTse, HF-
dc.contributor.authorDang, S-
dc.contributor.authorNie, Y-
dc.contributor.authorHuang, K-
dc.date.accessioned2021-11-12T13:35:14Z-
dc.date.available2021-11-12T13:35:14Z-
dc.date.issued2020-
dc.identifier.citationStem Cell Research, 2020, v. 49, article no. 102090-
dc.identifier.issn1873-5061-
dc.identifier.urihttp://hdl.handle.net/10722/307617-
dc.description.abstractGADD45A is a DNA damage and stressful growth arrest inducible protein, also it is shown to a be tumor suppressor gene and a chromatin relaxer associated with opening chromatin during the somatic reprogramming. However, its role in human embryonic stem cells and human embryonic stem cell modeled development has been merely documented. To illustrate the function of GADD45A in the human embryonic stem cell biology, we reported a GADD45A knockout human embryonic stem cell line by CRISPR/Cas9 mediated gene targeting. This cell line displayed normal karyotype, pluripotent stem cell marker expression and differentiation potential both in vivo and vitro.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at https://journals.elsevier.com/stem-cell-research-
dc.relation.ispartofStem Cell Research-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleGeneration of GADD45A gene knockout human embryonic stem cell line using CRISPR/Cas9-
dc.typeArticle-
dc.identifier.emailTse, HF: hftse@hkucc.hku.hk-
dc.identifier.authorityTse, HF=rp00428-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.scr.2020.102090-
dc.identifier.pmid33232900-
dc.identifier.scopuseid_2-s2.0-85096648354-
dc.identifier.hkuros329453-
dc.identifier.volume49-
dc.identifier.spagearticle no. 102090-
dc.identifier.epagearticle no. 102090-
dc.identifier.isiWOS:000600952800089-
dc.publisher.placeNetherlands-

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