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Article: Automated optical tweezers manipulation to transfer mitochondria from fetal to adult MSCs to improve antiaging gene expressions

TitleAutomated optical tweezers manipulation to transfer mitochondria from fetal to adult MSCs to improve antiaging gene expressions
Authors
Issue Date2021
PublisherWiley-VCH Verlag GmbH & Co KGaA. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-6829
Citation
Small, 2021, v. 17 n. 38, p. article no. 2103086 How to Cite?
AbstractMitochondrial dysfunction is considered to be an important factor that leads to aging and premature aging diseases. Transferring mitochondria to cells is an emerging and promising technique for the therapy of mitochondrial deoxyribonucleic acid (mtDNA)-related diseases. This paper presents a unique method of controlling the quality and quantity of mitochondria transferred to single cells using an automated optical tweezer-based micromanipulation system. The proposed method can automatically, accurately, and efficiently collect and transport healthy mitochondria to cells, and the recipient cells then take up the mitochondria through endocytosis. The results of the study reveal the possibility of using mitochondria from fetal mesenchymal stem cells (fMSCs) as a potential source to reverse the aging-related phenotype and improve metabolic activities in adult mesenchymal stem cells (aMSCs). The results of the quantitative polymerase chain reaction analysis show that the transfer of isolated mitochondria from fMSCs to a single aMSC can significantly increase the antiaging and metabolic gene expression in the aMSC. The proposed mitochondrial transfer method can greatly promote precision medicine for cell therapy of mtDNA-related diseases.
Persistent Identifierhttp://hdl.handle.net/10722/307730
ISSN
2023 Impact Factor: 13.0
2023 SCImago Journal Rankings: 3.348
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShakoor, A-
dc.contributor.authorWang, B-
dc.contributor.authorFan, L-
dc.contributor.authorKong, L-
dc.contributor.authorGao, W-
dc.contributor.authorSun, J-
dc.contributor.authorMan, K-
dc.contributor.authorLi, G-
dc.contributor.authorSun, D-
dc.date.accessioned2021-11-12T13:36:59Z-
dc.date.available2021-11-12T13:36:59Z-
dc.date.issued2021-
dc.identifier.citationSmall, 2021, v. 17 n. 38, p. article no. 2103086-
dc.identifier.issn1613-6810-
dc.identifier.urihttp://hdl.handle.net/10722/307730-
dc.description.abstractMitochondrial dysfunction is considered to be an important factor that leads to aging and premature aging diseases. Transferring mitochondria to cells is an emerging and promising technique for the therapy of mitochondrial deoxyribonucleic acid (mtDNA)-related diseases. This paper presents a unique method of controlling the quality and quantity of mitochondria transferred to single cells using an automated optical tweezer-based micromanipulation system. The proposed method can automatically, accurately, and efficiently collect and transport healthy mitochondria to cells, and the recipient cells then take up the mitochondria through endocytosis. The results of the study reveal the possibility of using mitochondria from fetal mesenchymal stem cells (fMSCs) as a potential source to reverse the aging-related phenotype and improve metabolic activities in adult mesenchymal stem cells (aMSCs). The results of the quantitative polymerase chain reaction analysis show that the transfer of isolated mitochondria from fMSCs to a single aMSC can significantly increase the antiaging and metabolic gene expression in the aMSC. The proposed mitochondrial transfer method can greatly promote precision medicine for cell therapy of mtDNA-related diseases.-
dc.languageeng-
dc.publisherWiley-VCH Verlag GmbH & Co KGaA. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-6829-
dc.relation.ispartofSmall-
dc.rightsSubmitted (preprint) Version This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Accepted (peer-reviewed) Version This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.titleAutomated optical tweezers manipulation to transfer mitochondria from fetal to adult MSCs to improve antiaging gene expressions-
dc.typeArticle-
dc.identifier.emailMan, K: kwanman@hku.hk-
dc.identifier.authorityMan, K=rp00417-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/smll.202103086-
dc.identifier.pmid34411428-
dc.identifier.scopuseid_2-s2.0-85112815641-
dc.identifier.hkuros330191-
dc.identifier.volume17-
dc.identifier.issue38-
dc.identifier.spagearticle no. 2103086-
dc.identifier.epagearticle no. 2103086-
dc.identifier.isiWOS:000686270900001-
dc.publisher.placeGermany-

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