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Article: Plasma polymer surface modified expanded polytetrafluoroethylene promotes epithelial monolayer formation in vitro and can be transplanted into the dystrophic rat subretinal space

TitlePlasma polymer surface modified expanded polytetrafluoroethylene promotes epithelial monolayer formation in vitro and can be transplanted into the dystrophic rat subretinal space
Authors
Issue Date2021
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www.wiley.com/WileyCDA/WileyTitle/productCd-TERM.html
Citation
Journal of Tissue Engineering and Regenerative Medicine, 2021, v. 15 n. 1, p. 49-62 How to Cite?
AbstractThe aim of this study was to evaluate whether the surface modification of expanded polytetrafluoroethylene (ePTFE) using an n-heptylamine (HA) plasma polymer would allow for functional epithelial monolayer formation suitable for subretinal transplant into a non-dystrophic rat model. Freshly isolated iris pigment epithelial (IPE) cells from two rat strains (Long Evans [LE] and Dark Agouti [DA]) were seeded onto HA, fibronectin-coated n-heptylamine modified (F-HA) and unmodified ePFTE and fibronectin-coated tissue culture (F-TCPS) substrates. Both F-HA ePTFE and F-TCPS substrates enabled functional monolayer formation with both strains of rat. Without fibronectin coating, only LE IPE formed a monolayer on HA-treated ePTFE. Functional assessment of both IPE strains on F-HA ePTFE demonstrated uptake of POS that increased significantly with time that was greater than control F-TCPS. Surgical optimization using Healon GV and mixtures of Healon GV: phosphate buffered saline (PBS) to induce retinal detachment demonstrated that only Healon GV:PBS allowed F-HA ePTFE substrates to be successfully transplanted into the subretinal space of Royal College of Surgeons rats, where they remained flat beneath the neural retina for up to 4 weeks. No apparent substrate-induced inflammatory response was observed by fundus microscopy or immunohistochemical analysis, indicating the potential of this substrate for future clinical applications.
Persistent Identifierhttp://hdl.handle.net/10722/307815
ISSN
2020 Impact Factor: 3.963
2020 SCImago Journal Rankings: 0.835
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNian, S-
dc.contributor.authorKearns, VR-
dc.contributor.authorWong, DSH-
dc.contributor.authorBachhuka, A-
dc.contributor.authorVasilev, K-
dc.contributor.authorWIlliams, RL-
dc.contributor.authorLai, WW-
dc.contributor.authorLo, A-
dc.contributor.authorSheridan, CM-
dc.date.accessioned2021-11-12T13:38:18Z-
dc.date.available2021-11-12T13:38:18Z-
dc.date.issued2021-
dc.identifier.citationJournal of Tissue Engineering and Regenerative Medicine, 2021, v. 15 n. 1, p. 49-62-
dc.identifier.issn1932-6254-
dc.identifier.urihttp://hdl.handle.net/10722/307815-
dc.description.abstractThe aim of this study was to evaluate whether the surface modification of expanded polytetrafluoroethylene (ePTFE) using an n-heptylamine (HA) plasma polymer would allow for functional epithelial monolayer formation suitable for subretinal transplant into a non-dystrophic rat model. Freshly isolated iris pigment epithelial (IPE) cells from two rat strains (Long Evans [LE] and Dark Agouti [DA]) were seeded onto HA, fibronectin-coated n-heptylamine modified (F-HA) and unmodified ePFTE and fibronectin-coated tissue culture (F-TCPS) substrates. Both F-HA ePTFE and F-TCPS substrates enabled functional monolayer formation with both strains of rat. Without fibronectin coating, only LE IPE formed a monolayer on HA-treated ePTFE. Functional assessment of both IPE strains on F-HA ePTFE demonstrated uptake of POS that increased significantly with time that was greater than control F-TCPS. Surgical optimization using Healon GV and mixtures of Healon GV: phosphate buffered saline (PBS) to induce retinal detachment demonstrated that only Healon GV:PBS allowed F-HA ePTFE substrates to be successfully transplanted into the subretinal space of Royal College of Surgeons rats, where they remained flat beneath the neural retina for up to 4 weeks. No apparent substrate-induced inflammatory response was observed by fundus microscopy or immunohistochemical analysis, indicating the potential of this substrate for future clinical applications.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www.wiley.com/WileyCDA/WileyTitle/productCd-TERM.html-
dc.relation.ispartofJournal of Tissue Engineering and Regenerative Medicine-
dc.rightsSubmitted (preprint) Version This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Accepted (peer-reviewed) Version This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.titlePlasma polymer surface modified expanded polytetrafluoroethylene promotes epithelial monolayer formation in vitro and can be transplanted into the dystrophic rat subretinal space-
dc.typeArticle-
dc.identifier.emailLo, A: amylo@hku.hk-
dc.identifier.authorityLo, A=rp00425-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/term.3154-
dc.identifier.scopuseid_2-s2.0-85096765705-
dc.identifier.hkuros329655-
dc.identifier.volume15-
dc.identifier.issue1-
dc.identifier.spage49-
dc.identifier.epage62-
dc.identifier.isiWOS:000591426900001-
dc.publisher.placeUnited States-

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