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Article: Impairment of caprine oocyte maturation in vitro and alteration of granulosa cells functions by widely used fungicide mancozeb

TitleImpairment of caprine oocyte maturation in vitro and alteration of granulosa cells functions by widely used fungicide mancozeb
Authors
KeywordsMancozeb
Cumulus oocyte complexes
Granulosa cells
Oocyte maturation
Progesterone
Issue Date2021
PublisherSpringer Netherlands. The Journal's web site is located at http://link.springer.com/journal/11250
Citation
Tropical Animal Health and Production, 2021, v. 53, p. article no. 406 How to Cite?
AbstractMancozeb is classified as an endocrine disruptor; thus the present study was carried out to investigate the impact of mancozeb on mammalian ovarian functions using in vitro caprine oocyte maturation and granulosa cell culture models. Caprine cumulus oocyte complexes (COCs) and granulosa cells were cultured under standard culture conditions and treated with mancozeb concentrations of 0.3, 3, and 30 μg/ml along with a control for 24 h and assessed. Granulosa cell viability and progesterone concentration in spent culture media after treatments were also assessed. Mancozeb significantly decreased (P < 0.05) the oocytes cumulus expansion and the maturation of caprine oocytes. Marked changes in granulose cell morphology were observed with 30 μg/ml mancozeb and significantly reduced (P < 0.05) cell viability. Interestingly, the same concentrations significantly increased (P < 0.05) the progesterone secretion by the cells. Significant reduction of granulosa cells viability and reduction of cumulus expansion and suppression of metaphase plate formation in oocyte can impair the fertilization ability and developmental potential of the oocytes. High progesterone concentration due to mancozeb treatment may suppress LH surge and suppress ovulation. In conclusion, mancozeb suppresses granulosa cells viability, reduces cumulus expansion, and suppresses metaphase plate formation but induces progesterone secretion from granulosa cells that may inhibit LH surge for ovulation process.
Persistent Identifierhttp://hdl.handle.net/10722/308180
ISSN
2023 Impact Factor: 1.7
2023 SCImago Journal Rankings: 0.481
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDinisri, I-
dc.contributor.authorKodikara, S-
dc.contributor.authorPrasadani, M-
dc.contributor.authorPathirana, I-
dc.contributor.authorRathnayake, C-
dc.contributor.authorAlexander, B-
dc.contributor.authorLee, KF-
dc.contributor.authorKodithuwakku Kankanamge, SPK-
dc.date.accessioned2021-11-12T13:43:36Z-
dc.date.available2021-11-12T13:43:36Z-
dc.date.issued2021-
dc.identifier.citationTropical Animal Health and Production, 2021, v. 53, p. article no. 406-
dc.identifier.issn0049-4747-
dc.identifier.urihttp://hdl.handle.net/10722/308180-
dc.description.abstractMancozeb is classified as an endocrine disruptor; thus the present study was carried out to investigate the impact of mancozeb on mammalian ovarian functions using in vitro caprine oocyte maturation and granulosa cell culture models. Caprine cumulus oocyte complexes (COCs) and granulosa cells were cultured under standard culture conditions and treated with mancozeb concentrations of 0.3, 3, and 30 μg/ml along with a control for 24 h and assessed. Granulosa cell viability and progesterone concentration in spent culture media after treatments were also assessed. Mancozeb significantly decreased (P < 0.05) the oocytes cumulus expansion and the maturation of caprine oocytes. Marked changes in granulose cell morphology were observed with 30 μg/ml mancozeb and significantly reduced (P < 0.05) cell viability. Interestingly, the same concentrations significantly increased (P < 0.05) the progesterone secretion by the cells. Significant reduction of granulosa cells viability and reduction of cumulus expansion and suppression of metaphase plate formation in oocyte can impair the fertilization ability and developmental potential of the oocytes. High progesterone concentration due to mancozeb treatment may suppress LH surge and suppress ovulation. In conclusion, mancozeb suppresses granulosa cells viability, reduces cumulus expansion, and suppresses metaphase plate formation but induces progesterone secretion from granulosa cells that may inhibit LH surge for ovulation process.-
dc.languageeng-
dc.publisherSpringer Netherlands. The Journal's web site is located at http://link.springer.com/journal/11250-
dc.relation.ispartofTropical Animal Health and Production-
dc.rightsThis version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/[insert DOI]-
dc.subjectMancozeb-
dc.subjectCumulus oocyte complexes-
dc.subjectGranulosa cells-
dc.subjectOocyte maturation-
dc.subjectProgesterone-
dc.titleImpairment of caprine oocyte maturation in vitro and alteration of granulosa cells functions by widely used fungicide mancozeb-
dc.typeArticle-
dc.identifier.emailLee, KF: ckflee@hku.hk-
dc.identifier.emailKodithuwakku Kankanamge, SPK: suranga@hku.hk-
dc.identifier.authorityLee, KF=rp00458-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11250-021-02854-5-
dc.identifier.pmid34287714-
dc.identifier.scopuseid_2-s2.0-85110634100-
dc.identifier.hkuros329647-
dc.identifier.volume53-
dc.identifier.spagearticle no. 406-
dc.identifier.epagearticle no. 406-
dc.identifier.isiWOS:000678662700001-
dc.publisher.placeNetherlands-

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