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Conference Paper: Molecules enhance neurogenic differentiation of dental-derived adult stem cells
Title | Molecules enhance neurogenic differentiation of dental-derived adult stem cells |
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Authors | |
Issue Date | 2019 |
Publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ |
Citation | The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec ISS A, Final Presentation ID: 3256 How to Cite? |
Abstract | Objectives: Dental pulp stem cells (DPSCs), stem cells from apical papilla (SCAPs) and gingival mesenchymal stem cells (GMSCs) exhibit high neurogenic potential. The aim of this study was therefore to utilize a cocktail of small molecules to enhance the neurogenic differentiation of DPSCs, SCAPs and GMSCs in vitro.
Methods: Neural induction of DPSCs, SCAPs and GMSCs was conducted by the cocktail of eight small molecules (CHIR99021, Dorsomorphin, Forskolin, GO6983, Repsox, SP600125, Valproic acid, and Y-27632 ) over a duration of 14 days. The derived putative neural lineage cells were analysed by qRT-PCR, Western blotting, immunocytochemistry and the Fluo-4 AM calcium flux assay.
Results: After 14 days of neural induction by the cocktail of small molecules, distinct morphological characteristic changes of the neural lineage were observed in all three dental-derived adult stem cells. Conversely, much less marked morphological changes were displayed by the corresponding untreated controls. As shown by qRT-PCR analyses, DPSCs and SCAPs treated with small molecules demonstrated significantly increased expression of mature neural markers NSE and NFM, as compared with the control groups (all p<0.05). However, GMSCs treated with small molecules exhibited significantly increased expression of early neural markers such as βIII-tubulin, Musashi 1, MASH1, and NGN2 (all p<0.05). The immunostaining data and western blot analysis further indicated that only significant upregulation of NSE expression by all three chemical-induced neural lineages compared with the corresponding untreated control. Consistently, the calcium transient (F/Fo) peaks for the cells treated with small molecules were significantly higher than the corresponding untreated control as indicated by Fluo-4 AM calcium flux assay (all p<0.05).
Conclusions: The small-molecule cocktail enhance the commitment and differentiation of DPSCs, SCAPs and GMSCs into the neural lineage. |
Description | Poster Session: New Insights into Bruxism, Sleep Apnea and Related Disorders - Final Presentation ID: 3256 |
Persistent Identifier | http://hdl.handle.net/10722/308260 |
DC Field | Value | Language |
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dc.contributor.author | Jiang, S | - |
dc.contributor.author | Heng, BC | - |
dc.contributor.author | Zhang, C | - |
dc.date.accessioned | 2021-11-12T13:44:44Z | - |
dc.date.available | 2021-11-12T13:44:44Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec ISS A, Final Presentation ID: 3256 | - |
dc.identifier.uri | http://hdl.handle.net/10722/308260 | - |
dc.description | Poster Session: New Insights into Bruxism, Sleep Apnea and Related Disorders - Final Presentation ID: 3256 | - |
dc.description.abstract | Objectives: Dental pulp stem cells (DPSCs), stem cells from apical papilla (SCAPs) and gingival mesenchymal stem cells (GMSCs) exhibit high neurogenic potential. The aim of this study was therefore to utilize a cocktail of small molecules to enhance the neurogenic differentiation of DPSCs, SCAPs and GMSCs in vitro. Methods: Neural induction of DPSCs, SCAPs and GMSCs was conducted by the cocktail of eight small molecules (CHIR99021, Dorsomorphin, Forskolin, GO6983, Repsox, SP600125, Valproic acid, and Y-27632 ) over a duration of 14 days. The derived putative neural lineage cells were analysed by qRT-PCR, Western blotting, immunocytochemistry and the Fluo-4 AM calcium flux assay. Results: After 14 days of neural induction by the cocktail of small molecules, distinct morphological characteristic changes of the neural lineage were observed in all three dental-derived adult stem cells. Conversely, much less marked morphological changes were displayed by the corresponding untreated controls. As shown by qRT-PCR analyses, DPSCs and SCAPs treated with small molecules demonstrated significantly increased expression of mature neural markers NSE and NFM, as compared with the control groups (all p<0.05). However, GMSCs treated with small molecules exhibited significantly increased expression of early neural markers such as βIII-tubulin, Musashi 1, MASH1, and NGN2 (all p<0.05). The immunostaining data and western blot analysis further indicated that only significant upregulation of NSE expression by all three chemical-induced neural lineages compared with the corresponding untreated control. Consistently, the calcium transient (F/Fo) peaks for the cells treated with small molecules were significantly higher than the corresponding untreated control as indicated by Fluo-4 AM calcium flux assay (all p<0.05). Conclusions: The small-molecule cocktail enhance the commitment and differentiation of DPSCs, SCAPs and GMSCs into the neural lineage. | - |
dc.language | eng | - |
dc.publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ | - |
dc.relation.ispartof | Journal of Dental Research (Spec Issue) | - |
dc.relation.ispartof | IADR/AADR/CADR 2019 General Session & Exhibition | - |
dc.title | Molecules enhance neurogenic differentiation of dental-derived adult stem cells | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.description.nature | abstract | - |
dc.identifier.hkuros | 329477 | - |
dc.identifier.volume | 98 | - |
dc.identifier.issue | Spec ISS A | - |
dc.identifier.spage | Final Presentation ID: 3256 | - |
dc.identifier.epage | Final Presentation ID: 3256 | - |
dc.publisher.place | United States | - |