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Conference Paper: Molecules enhance neurogenic differentiation of dental-derived adult stem cells

TitleMolecules enhance neurogenic differentiation of dental-derived adult stem cells
Authors
Issue Date2019
PublisherInternational Association for Dental Research. The Journal's web site is located at http://www.iadr.org/
Citation
The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec ISS A, Final Presentation ID: 3256 How to Cite?
AbstractObjectives: Dental pulp stem cells (DPSCs), stem cells from apical papilla (SCAPs) and gingival mesenchymal stem cells (GMSCs) exhibit high neurogenic potential. The aim of this study was therefore to utilize a cocktail of small molecules to enhance the neurogenic differentiation of DPSCs, SCAPs and GMSCs in vitro. Methods: Neural induction of DPSCs, SCAPs and GMSCs was conducted by the cocktail of eight small molecules (CHIR99021, Dorsomorphin, Forskolin, GO6983, Repsox, SP600125, Valproic acid, and Y-27632 ) over a duration of 14 days. The derived putative neural lineage cells were analysed by qRT-PCR, Western blotting, immunocytochemistry and the Fluo-4 AM calcium flux assay. Results: After 14 days of neural induction by the cocktail of small molecules, distinct morphological characteristic changes of the neural lineage were observed in all three dental-derived adult stem cells. Conversely, much less marked morphological changes were displayed by the corresponding untreated controls. As shown by qRT-PCR analyses, DPSCs and SCAPs treated with small molecules demonstrated significantly increased expression of mature neural markers NSE and NFM, as compared with the control groups (all p<0.05). However, GMSCs treated with small molecules exhibited significantly increased expression of early neural markers such as βIII-tubulin, Musashi 1, MASH1, and NGN2 (all p<0.05). The immunostaining data and western blot analysis further indicated that only significant upregulation of NSE expression by all three chemical-induced neural lineages compared with the corresponding untreated control. Consistently, the calcium transient (F/Fo) peaks for the cells treated with small molecules were significantly higher than the corresponding untreated control as indicated by Fluo-4 AM calcium flux assay (all p<0.05). Conclusions: The small-molecule cocktail enhance the commitment and differentiation of DPSCs, SCAPs and GMSCs into the neural lineage.
DescriptionPoster Session: New Insights into Bruxism, Sleep Apnea and Related Disorders - Final Presentation ID: 3256
Persistent Identifierhttp://hdl.handle.net/10722/308260

 

DC FieldValueLanguage
dc.contributor.authorJiang, S-
dc.contributor.authorHeng, BC-
dc.contributor.authorZhang, C-
dc.date.accessioned2021-11-12T13:44:44Z-
dc.date.available2021-11-12T13:44:44Z-
dc.date.issued2019-
dc.identifier.citationThe 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec ISS A, Final Presentation ID: 3256-
dc.identifier.urihttp://hdl.handle.net/10722/308260-
dc.descriptionPoster Session: New Insights into Bruxism, Sleep Apnea and Related Disorders - Final Presentation ID: 3256-
dc.description.abstractObjectives: Dental pulp stem cells (DPSCs), stem cells from apical papilla (SCAPs) and gingival mesenchymal stem cells (GMSCs) exhibit high neurogenic potential. The aim of this study was therefore to utilize a cocktail of small molecules to enhance the neurogenic differentiation of DPSCs, SCAPs and GMSCs in vitro. Methods: Neural induction of DPSCs, SCAPs and GMSCs was conducted by the cocktail of eight small molecules (CHIR99021, Dorsomorphin, Forskolin, GO6983, Repsox, SP600125, Valproic acid, and Y-27632 ) over a duration of 14 days. The derived putative neural lineage cells were analysed by qRT-PCR, Western blotting, immunocytochemistry and the Fluo-4 AM calcium flux assay. Results: After 14 days of neural induction by the cocktail of small molecules, distinct morphological characteristic changes of the neural lineage were observed in all three dental-derived adult stem cells. Conversely, much less marked morphological changes were displayed by the corresponding untreated controls. As shown by qRT-PCR analyses, DPSCs and SCAPs treated with small molecules demonstrated significantly increased expression of mature neural markers NSE and NFM, as compared with the control groups (all p<0.05). However, GMSCs treated with small molecules exhibited significantly increased expression of early neural markers such as βIII-tubulin, Musashi 1, MASH1, and NGN2 (all p<0.05). The immunostaining data and western blot analysis further indicated that only significant upregulation of NSE expression by all three chemical-induced neural lineages compared with the corresponding untreated control. Consistently, the calcium transient (F/Fo) peaks for the cells treated with small molecules were significantly higher than the corresponding untreated control as indicated by Fluo-4 AM calcium flux assay (all p<0.05). Conclusions: The small-molecule cocktail enhance the commitment and differentiation of DPSCs, SCAPs and GMSCs into the neural lineage.-
dc.languageeng-
dc.publisherInternational Association for Dental Research. The Journal's web site is located at http://www.iadr.org/-
dc.relation.ispartofJournal of Dental Research (Spec Issue)-
dc.relation.ispartofIADR/AADR/CADR 2019 General Session & Exhibition-
dc.titleMolecules enhance neurogenic differentiation of dental-derived adult stem cells-
dc.typeConference_Paper-
dc.identifier.emailZhang, C: zhangcf@hku.hk-
dc.identifier.authorityZhang, C=rp01408-
dc.description.natureabstract-
dc.identifier.hkuros329477-
dc.identifier.volume98-
dc.identifier.issueSpec ISS A-
dc.identifier.spageFinal Presentation ID: 3256-
dc.identifier.epageFinal Presentation ID: 3256-
dc.publisher.placeUnited States-

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