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- Publisher Website: 10.1109/TNB.2021.3096056
- Scopus: eid_2-s2.0-85112602944
- PMID: 34242170
- WOS: WOS:000702562200017
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Article: Hierarchical Micro-/Nanotopography for Tuning Structures and Mechanics of Cells Probed by Atomic Force Microscopy
Title | Hierarchical Micro-/Nanotopography for Tuning Structures and Mechanics of Cells Probed by Atomic Force Microscopy |
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Authors | |
Keywords | Atomic force microscopy cell-matrix interaction microgroove substrate nanogranular surface cell mechanics |
Issue Date | 2021 |
Publisher | Institute of Electrical and Electronics Engineers. The Journal's web site is located at https://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=7728 |
Citation | IEEE Transactions on NanoBioscience, 2021, v. 20 n. 4, p. 543-553 How to Cite? |
Abstract | Extracellular matrix plays an important role in regulating the behaviors of cells, and utilizing matrix physics to control cell fate has been a promising way for cell and tissue engineering. However, the nanoscale situations taking place during the topography-regulated cell-matrix interactions are still not fully understood to the best of our knowledge. The invention of atomic force microscopy (AFM) provides a powerful tool to characterize the structures and properties of living biological systems under aqueous conditions with unprecedented spatial resolution. In this work, with the use of AFM, structural and mechanical dynamics of individual cells grown on micro-/nanotopographical surface were revealed. First, the microgroove patterned silicon substrates were fabricated by photolithography. Next, nanogranular topography was formed on microgroove substrates by cell culture medium protein deposition, which was visualized by in situ AFM imaging. The micro-/nanotopographical substrates were then used to grow two types of cells (3T3 cell or MCF-7 cell). AFM morphological imaging and mechanical measurements were applied to characterize the changes of cells grown on the micro-/nanotopographical substrates. The experimental results showed the significant alterations in cellular structures and cellular mechanics caused by micro-/nanotopography. The study provides a novel way based on AFM to unveil the native nanostructures and mechanical properties of cell-matrix interfaces with high spatial resolution in liquids, which will have potential impacts on the studies of topography-tuned cell behaviors. |
Persistent Identifier | http://hdl.handle.net/10722/309386 |
ISSN | 2023 Impact Factor: 3.7 2023 SCImago Journal Rankings: 0.659 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, M | - |
dc.contributor.author | Xi, N | - |
dc.contributor.author | Liu, L | - |
dc.date.accessioned | 2021-12-29T02:14:22Z | - |
dc.date.available | 2021-12-29T02:14:22Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | IEEE Transactions on NanoBioscience, 2021, v. 20 n. 4, p. 543-553 | - |
dc.identifier.issn | 1536-1241 | - |
dc.identifier.uri | http://hdl.handle.net/10722/309386 | - |
dc.description.abstract | Extracellular matrix plays an important role in regulating the behaviors of cells, and utilizing matrix physics to control cell fate has been a promising way for cell and tissue engineering. However, the nanoscale situations taking place during the topography-regulated cell-matrix interactions are still not fully understood to the best of our knowledge. The invention of atomic force microscopy (AFM) provides a powerful tool to characterize the structures and properties of living biological systems under aqueous conditions with unprecedented spatial resolution. In this work, with the use of AFM, structural and mechanical dynamics of individual cells grown on micro-/nanotopographical surface were revealed. First, the microgroove patterned silicon substrates were fabricated by photolithography. Next, nanogranular topography was formed on microgroove substrates by cell culture medium protein deposition, which was visualized by in situ AFM imaging. The micro-/nanotopographical substrates were then used to grow two types of cells (3T3 cell or MCF-7 cell). AFM morphological imaging and mechanical measurements were applied to characterize the changes of cells grown on the micro-/nanotopographical substrates. The experimental results showed the significant alterations in cellular structures and cellular mechanics caused by micro-/nanotopography. The study provides a novel way based on AFM to unveil the native nanostructures and mechanical properties of cell-matrix interfaces with high spatial resolution in liquids, which will have potential impacts on the studies of topography-tuned cell behaviors. | - |
dc.language | eng | - |
dc.publisher | Institute of Electrical and Electronics Engineers. The Journal's web site is located at https://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=7728 | - |
dc.relation.ispartof | IEEE Transactions on NanoBioscience | - |
dc.rights | IEEE Transactions on NanoBioscience. Copyright © Institute of Electrical and Electronics Engineers. | - |
dc.rights | ©20xx IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. | - |
dc.subject | Atomic force microscopy | - |
dc.subject | cell-matrix interaction | - |
dc.subject | microgroove substrate | - |
dc.subject | nanogranular surface | - |
dc.subject | cell mechanics | - |
dc.title | Hierarchical Micro-/Nanotopography for Tuning Structures and Mechanics of Cells Probed by Atomic Force Microscopy | - |
dc.type | Article | - |
dc.identifier.email | Xi, N: xining@hku.hk | - |
dc.identifier.authority | Xi, N=rp02044 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1109/TNB.2021.3096056 | - |
dc.identifier.pmid | 34242170 | - |
dc.identifier.scopus | eid_2-s2.0-85112602944 | - |
dc.identifier.hkuros | 331228 | - |
dc.identifier.volume | 20 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 543 | - |
dc.identifier.epage | 553 | - |
dc.identifier.isi | WOS:000702562200017 | - |
dc.publisher.place | United States | - |