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Article: Deficiency in flavonoid biosynthesis genes CHS, CHI, and CHIL alters rice flavonoid and lignin profiles

TitleDeficiency in flavonoid biosynthesis genes CHS, CHI, and CHIL alters rice flavonoid and lignin profiles
Authors
Issue Date2022
PublisherAmerican Society of Plant Physiologists. The Journal's web site is located at http://www.plantphysiol.org
Citation
Plant Physiology, 2022, v. 188 n. 4, p. 1993-2011 How to Cite?
AbstractLignin is a complex phenylpropanoid polymer deposited in the secondary cell walls of vascular plants. Unlike most gymnosperm and eudicot lignins that are generated via the polymerization of monolignols, grass lignins additionally incorporate the flavonoid tricin as a natural lignin monomer. The biosynthesis and functions of tricin-integrated lignin (tricin-lignin) in grass cell walls and its effects on the utility of grass biomass remain largely unknown. We herein report a comparative analysis of rice (Oryza sativa) mutants deficient in the early flavonoid biosynthetic genes encoding CHALCONE SYNTHASE (CHS), CHALCONE ISOMERASE (CHI), and CHI-LIKE (CHIL), with an emphasis on the analyses of disrupted tricin-lignin formation and the concurrent changes in lignin profiles and cell wall digestibility. All examined CHS-, CHI-, and CHIL-deficient rice mutants were largely depleted of extractable flavones, including tricin, and nearly devoid of tricin-lignin in the cell walls, supporting the crucial roles of CHS and CHI as committed enzymes and CHIL as a noncatalytic enhancer in the conserved biosynthetic pathway leading to flavone and tricin-lignin formation. In-depth cell wall structural analyses further indicated that lignin content and composition, including the monolignol-derived units, were differentially altered in the mutants. However, regardless of the extent of the lignin alterations, cell wall saccharification efficiencies of all tested rice mutants were similar to that of the wild-type controls. Together with earlier studies on other tricin-depleted grass mutant and transgenic plants, our results reflect the complexity in the metabolic consequences of tricin pathway perturbations and the relationships between lignin profiles and cell wall properties.
Persistent Identifierhttp://hdl.handle.net/10722/310098
ISSN
2023 Impact Factor: 6.5
2023 SCImago Journal Rankings: 2.101
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLam, PY-
dc.contributor.authorWANG, L-
dc.contributor.authorLUI, CW-
dc.contributor.authorLiu, H-
dc.contributor.authorTakeda-Kimura, Y-
dc.contributor.authorChen, MX-
dc.contributor.authorZhu, FY-
dc.contributor.authorZhang, J-
dc.contributor.authorUmezawa, T-
dc.contributor.authorTobimatsu, Y-
dc.contributor.authorLo, CSC-
dc.date.accessioned2022-01-24T02:23:48Z-
dc.date.available2022-01-24T02:23:48Z-
dc.date.issued2022-
dc.identifier.citationPlant Physiology, 2022, v. 188 n. 4, p. 1993-2011-
dc.identifier.issn0032-0889-
dc.identifier.urihttp://hdl.handle.net/10722/310098-
dc.description.abstractLignin is a complex phenylpropanoid polymer deposited in the secondary cell walls of vascular plants. Unlike most gymnosperm and eudicot lignins that are generated via the polymerization of monolignols, grass lignins additionally incorporate the flavonoid tricin as a natural lignin monomer. The biosynthesis and functions of tricin-integrated lignin (tricin-lignin) in grass cell walls and its effects on the utility of grass biomass remain largely unknown. We herein report a comparative analysis of rice (Oryza sativa) mutants deficient in the early flavonoid biosynthetic genes encoding CHALCONE SYNTHASE (CHS), CHALCONE ISOMERASE (CHI), and CHI-LIKE (CHIL), with an emphasis on the analyses of disrupted tricin-lignin formation and the concurrent changes in lignin profiles and cell wall digestibility. All examined CHS-, CHI-, and CHIL-deficient rice mutants were largely depleted of extractable flavones, including tricin, and nearly devoid of tricin-lignin in the cell walls, supporting the crucial roles of CHS and CHI as committed enzymes and CHIL as a noncatalytic enhancer in the conserved biosynthetic pathway leading to flavone and tricin-lignin formation. In-depth cell wall structural analyses further indicated that lignin content and composition, including the monolignol-derived units, were differentially altered in the mutants. However, regardless of the extent of the lignin alterations, cell wall saccharification efficiencies of all tested rice mutants were similar to that of the wild-type controls. Together with earlier studies on other tricin-depleted grass mutant and transgenic plants, our results reflect the complexity in the metabolic consequences of tricin pathway perturbations and the relationships between lignin profiles and cell wall properties.-
dc.languageeng-
dc.publisherAmerican Society of Plant Physiologists. The Journal's web site is located at http://www.plantphysiol.org-
dc.relation.ispartofPlant Physiology-
dc.titleDeficiency in flavonoid biosynthesis genes CHS, CHI, and CHIL alters rice flavonoid and lignin profiles-
dc.typeArticle-
dc.identifier.emailLo, CSC: clivelo@hku.hk-
dc.identifier.authorityLo, CSC=rp00751-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/plphys/kiab606-
dc.identifier.pmid34963002-
dc.identifier.pmcidPMC8969032-
dc.identifier.hkuros331436-
dc.identifier.volume188-
dc.identifier.issue4-
dc.identifier.spage1993-
dc.identifier.epage2011-
dc.identifier.isiWOS:000764692400001-
dc.publisher.placeUnited States-

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