File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3390/ijms22094486
- Scopus: eid_2-s2.0-85104572079
- PMID: 33925774
- WOS: WOS:000650393000001
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: PDLCs and EPCs co-cultured on Ta discs: A golden fleece for 'compromised' osseointegration
Title | PDLCs and EPCs co-cultured on Ta discs: A golden fleece for 'compromised' osseointegration |
---|---|
Authors | |
Keywords | osteogenesis neovascularization osseointegration VEGFR-2 RUNX-2 |
Issue Date | 2021 |
Publisher | Molecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms |
Citation | International Journal of Molecular Sciences, 2021, v. 22 n. 9, p. article no. 4486 How to Cite? |
Abstract | Material research in tissue engineering forms a vital link between basic cell research and animal research. Periodontal ligament cells (PDLCs, P) from the tooth have an osteogenic effect, whereas endothelial progenitor cells (EPCs, E) assist in neovascularization. In the present study, the interaction of PDLCs and EPCs with Tantalum (Ta, I) discs, either alone (IP or IE group) or in 1:1 (IPE) ratio was explored. Additionally, surface analysis of Ta discs with respect to different types and cycles of sterilization and disinfection procedures was evaluated. It was observed that Ta discs could be used for a maximum of three times, after which the changes in properties of Ta discs were detrimental to cell growth, irrespective of the type of the protocol. Cell-Disc’s analysis revealed that cell proliferation in the IE group at day 6 and day 10 was significantly higher (p < 0.05) than other groups. A cell viability assay revealed increased live cells in the IPE group than in the IP or IE group. Similarly, adhesion and penetration of cells in the IPE group were not only higher, but also had an increased thickness of cellular extensions. RT-PCR analysis revealed that on day 8, both osteogenic (ALP, RUNX-2, and BSP) and angiogenic genes (VEGFR-2, CD31) increased significantly in the IPE group as compared to the IP or IE groups (p < 0.05). In conclusion, Ta discs promoted cellular proliferation and increased osteogenic and angiogenic activity by augmenting RUNX-2 and VEGFR-2 activity. |
Persistent Identifier | http://hdl.handle.net/10722/310583 |
ISSN | 2023 Impact Factor: 4.9 2023 SCImago Journal Rankings: 1.179 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chopra, H | - |
dc.contributor.author | Han, Y | - |
dc.contributor.author | Zhang, C | - |
dc.contributor.author | Pow, EHN | - |
dc.date.accessioned | 2022-02-07T07:58:49Z | - |
dc.date.available | 2022-02-07T07:58:49Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | International Journal of Molecular Sciences, 2021, v. 22 n. 9, p. article no. 4486 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/10722/310583 | - |
dc.description.abstract | Material research in tissue engineering forms a vital link between basic cell research and animal research. Periodontal ligament cells (PDLCs, P) from the tooth have an osteogenic effect, whereas endothelial progenitor cells (EPCs, E) assist in neovascularization. In the present study, the interaction of PDLCs and EPCs with Tantalum (Ta, I) discs, either alone (IP or IE group) or in 1:1 (IPE) ratio was explored. Additionally, surface analysis of Ta discs with respect to different types and cycles of sterilization and disinfection procedures was evaluated. It was observed that Ta discs could be used for a maximum of three times, after which the changes in properties of Ta discs were detrimental to cell growth, irrespective of the type of the protocol. Cell-Disc’s analysis revealed that cell proliferation in the IE group at day 6 and day 10 was significantly higher (p < 0.05) than other groups. A cell viability assay revealed increased live cells in the IPE group than in the IP or IE group. Similarly, adhesion and penetration of cells in the IPE group were not only higher, but also had an increased thickness of cellular extensions. RT-PCR analysis revealed that on day 8, both osteogenic (ALP, RUNX-2, and BSP) and angiogenic genes (VEGFR-2, CD31) increased significantly in the IPE group as compared to the IP or IE groups (p < 0.05). In conclusion, Ta discs promoted cellular proliferation and increased osteogenic and angiogenic activity by augmenting RUNX-2 and VEGFR-2 activity. | - |
dc.language | eng | - |
dc.publisher | Molecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms | - |
dc.relation.ispartof | International Journal of Molecular Sciences | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | osteogenesis | - |
dc.subject | neovascularization | - |
dc.subject | osseointegration | - |
dc.subject | VEGFR-2 | - |
dc.subject | RUNX-2 | - |
dc.title | PDLCs and EPCs co-cultured on Ta discs: A golden fleece for 'compromised' osseointegration | - |
dc.type | Article | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.email | Pow, EHN: ehnpow@hku.hk | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.identifier.authority | Pow, EHN=rp00030 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/ijms22094486 | - |
dc.identifier.pmid | 33925774 | - |
dc.identifier.pmcid | PMC8123461 | - |
dc.identifier.scopus | eid_2-s2.0-85104572079 | - |
dc.identifier.hkuros | 331654 | - |
dc.identifier.volume | 22 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | article no. 4486 | - |
dc.identifier.epage | article no. 4486 | - |
dc.identifier.isi | WOS:000650393000001 | - |
dc.publisher.place | Switzerland | - |