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- Publisher Website: 10.1083/jcb.201108152
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- PMID: 22472441
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Article: Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism
Title | Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism |
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Authors | |
Issue Date | 2012 |
Citation | Journal of Cell Biology, 2012, v. 197, n. 1, p. 105-114 How to Cite? |
Abstract | Mesenchymal cell migration as exhibited by fibroblasts is distinct from amoeboid cell migration and is characterized by dynamic competition among multiple protrusions, which determines directional persistence and responses to spatial cues. Localization of phosphoinositide 3-kinase (PI3K) signaling is thought to play a broadly important role in cell motility, yet the context-dependent functions of this pathway have not been adequately elucidated. By mapping the spatiotemporal dynamics of cell protrusion/retraction and PI3K signaling monitored by total internal reflection fluorescence microscopy, we show that randomly migrating fibroblasts reorient polarity through PI3K-dependent branching and pivoting of protrusions. PI3K inhibition did not affect the initiation of newly branched protrusions, nor did it prevent protrusion induced by photoactivation of Rac. Rather, PI3K signaling increased after, not before, the onset of local protrusion and was required for the lateral spreading and stabilization of nascent branches. During chemotaxis, the branch experiencing the higher chemoattractant concentration was favored, and, thus, the cell reoriented so as to align with the external gradient. © 2012 Welf et al. |
Persistent Identifier | http://hdl.handle.net/10722/311371 |
ISSN | 2023 Impact Factor: 7.4 2023 SCImago Journal Rankings: 3.717 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Welf, Erik S. | - |
dc.contributor.author | Ahmed, Shoeb | - |
dc.contributor.author | Johnson, Heath E. | - |
dc.contributor.author | Melvin, Adam T. | - |
dc.contributor.author | Haugh, Jason M. | - |
dc.date.accessioned | 2022-03-22T11:53:46Z | - |
dc.date.available | 2022-03-22T11:53:46Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Journal of Cell Biology, 2012, v. 197, n. 1, p. 105-114 | - |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.uri | http://hdl.handle.net/10722/311371 | - |
dc.description.abstract | Mesenchymal cell migration as exhibited by fibroblasts is distinct from amoeboid cell migration and is characterized by dynamic competition among multiple protrusions, which determines directional persistence and responses to spatial cues. Localization of phosphoinositide 3-kinase (PI3K) signaling is thought to play a broadly important role in cell motility, yet the context-dependent functions of this pathway have not been adequately elucidated. By mapping the spatiotemporal dynamics of cell protrusion/retraction and PI3K signaling monitored by total internal reflection fluorescence microscopy, we show that randomly migrating fibroblasts reorient polarity through PI3K-dependent branching and pivoting of protrusions. PI3K inhibition did not affect the initiation of newly branched protrusions, nor did it prevent protrusion induced by photoactivation of Rac. Rather, PI3K signaling increased after, not before, the onset of local protrusion and was required for the lateral spreading and stabilization of nascent branches. During chemotaxis, the branch experiencing the higher chemoattractant concentration was favored, and, thus, the cell reoriented so as to align with the external gradient. © 2012 Welf et al. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Cell Biology | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1083/jcb.201108152 | - |
dc.identifier.pmid | 22472441 | - |
dc.identifier.pmcid | PMC3317800 | - |
dc.identifier.scopus | eid_2-s2.0-84860295783 | - |
dc.identifier.volume | 197 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 105 | - |
dc.identifier.epage | 114 | - |
dc.identifier.eissn | 1540-8140 | - |
dc.identifier.isi | WOS:000302476600012 | - |