File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1002/0471143030.cb1414s61
- Scopus: eid_2-s2.0-84896330579
- PMID: 24510804
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Quantitative analysis of phosphoinositide 3-kinase (PI3K) signaling using live-cell total internal reflection fluorescence (TIRF) microscopy
Title | Quantitative analysis of phosphoinositide 3-kinase (PI3K) signaling using live-cell total internal reflection fluorescence (TIRF) microscopy |
---|---|
Authors | |
Keywords | Cell biophysics Cell migration Image analysis Image processing Signal transduction |
Issue Date | 2013 |
Citation | Current Protocols in Cell Biology, 2013, suppl. 61, p. 14.14.1-14.14.24 How to Cite? |
Abstract | This unit focuses on the use of total internal reflection fluorescence (TIRF) microscopy and image analysis methods to study the dynamics of signal transduction mediated by class I phosphoinositide 3-kinases (PI3Ks) in mammalian cells. The first four protocols cover live-cell imaging experiments, image acquisition parameters, and basic image processing and segmentation. These methods are generally applicable to livecell TIRF experiments. The remaining protocols outline more advanced image analysis methods, which were developed in our laboratory for the purpose of characterizing the spatiotemporal dynamics of PI3K signaling. These methods may be extended to analyze other cellular processes monitored using fluorescent biosensors. © 2013 by John Wiley & Sons, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/311385 |
ISSN | 2023 SCImago Journal Rankings: 1.872 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Johnson, Heath E. | - |
dc.contributor.author | Haugh, Jason M. | - |
dc.date.accessioned | 2022-03-22T11:53:48Z | - |
dc.date.available | 2022-03-22T11:53:48Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Current Protocols in Cell Biology, 2013, suppl. 61, p. 14.14.1-14.14.24 | - |
dc.identifier.issn | 1934-2500 | - |
dc.identifier.uri | http://hdl.handle.net/10722/311385 | - |
dc.description.abstract | This unit focuses on the use of total internal reflection fluorescence (TIRF) microscopy and image analysis methods to study the dynamics of signal transduction mediated by class I phosphoinositide 3-kinases (PI3Ks) in mammalian cells. The first four protocols cover live-cell imaging experiments, image acquisition parameters, and basic image processing and segmentation. These methods are generally applicable to livecell TIRF experiments. The remaining protocols outline more advanced image analysis methods, which were developed in our laboratory for the purpose of characterizing the spatiotemporal dynamics of PI3K signaling. These methods may be extended to analyze other cellular processes monitored using fluorescent biosensors. © 2013 by John Wiley & Sons, Inc. | - |
dc.language | eng | - |
dc.relation.ispartof | Current Protocols in Cell Biology | - |
dc.subject | Cell biophysics | - |
dc.subject | Cell migration | - |
dc.subject | Image analysis | - |
dc.subject | Image processing | - |
dc.subject | Signal transduction | - |
dc.title | Quantitative analysis of phosphoinositide 3-kinase (PI3K) signaling using live-cell total internal reflection fluorescence (TIRF) microscopy | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/0471143030.cb1414s61 | - |
dc.identifier.pmid | 24510804 | - |
dc.identifier.scopus | eid_2-s2.0-84896330579 | - |
dc.identifier.issue | suppl. 61 | - |
dc.identifier.spage | 14.14.1 | - |
dc.identifier.epage | 14.14.24 | - |
dc.identifier.eissn | 1934-2616 | - |