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Article: Impact of Adjuvants on the Immunogenicity and Efficacy of Split-Virion H7N9 Vaccine in Ferrets

TitleImpact of Adjuvants on the Immunogenicity and Efficacy of Split-Virion H7N9 Vaccine in Ferrets
Authors
Keywordsadjuvants
ferrets
H7N9
influenza
vaccine
Issue Date2015
Citation
Journal of Infectious Diseases, 2015, v. 212, n. 4, p. 542-551 How to Cite?
AbstractBackground. An effective vaccine is urgently needed against the H7N9 avian influenza virus. We evaluated the immunogenicity and protective efficacy of a split-virion H7N9 vaccine with or without the oil-in-water adjuvants in ferrets. Methods. Ferrets were vaccinated with 2 doses of unadjuvanted, MF59 or AS03-adjuvanted A/Shanghai/2/2013 (H7N9) vaccine, and the induction of antibodies to hemagglutinin (HA) or neuraminidase proteins was evaluated. Ferrets were then challenged with wild-type H7N9 virus to assess the vaccine's protective efficacy. The vaccine composition and integrity was also evaluated in vitro. Results. Adjuvanted vaccines stimulated robust serum antibody titers against HA and neuraminidase compared with the unadjuvanted vaccines. Although there was a difference in adjuvanticity between AS03 and MF59 at a lower dose (3.75 μg of HA), both adjuvants induced comparable antibody responses after 2 doses of 15 μg. On challenge, ferrets that received adjuvanted vaccines showed lower viral burden than the control or unadjuvanted vaccine group. In vitro examinations revealed that the vaccine contained visible split-virus particles and retained the native conformation of HA recognizable by polyclonal and monoclonal antibodies. Conclusions. The adjuvanted H7N9 vaccines demonstrated superior immunogenicity and protective efficacy against H7N9 infection in ferrets and hold potential as a vaccination regimen.
Persistent Identifierhttp://hdl.handle.net/10722/312003
ISSN
2023 Impact Factor: 5.0
2023 SCImago Journal Rankings: 2.387
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, Sook San-
dc.contributor.authorKaplan, Bryan-
dc.contributor.authorZanin, Mark-
dc.contributor.authorDebeauchamp, Jennifer-
dc.contributor.authorKercher, Lisa-
dc.contributor.authorCrumpton, Jeri Carol-
dc.contributor.authorSeiler, Patrick-
dc.contributor.authorSun, Yilun-
dc.contributor.authorTang, Li-
dc.contributor.authorKrauss, Scott-
dc.contributor.authorWebster, Robert-
dc.contributor.authorWebby, Richard J.-
dc.date.accessioned2022-04-06T04:31:57Z-
dc.date.available2022-04-06T04:31:57Z-
dc.date.issued2015-
dc.identifier.citationJournal of Infectious Diseases, 2015, v. 212, n. 4, p. 542-551-
dc.identifier.issn0022-1899-
dc.identifier.urihttp://hdl.handle.net/10722/312003-
dc.description.abstractBackground. An effective vaccine is urgently needed against the H7N9 avian influenza virus. We evaluated the immunogenicity and protective efficacy of a split-virion H7N9 vaccine with or without the oil-in-water adjuvants in ferrets. Methods. Ferrets were vaccinated with 2 doses of unadjuvanted, MF59 or AS03-adjuvanted A/Shanghai/2/2013 (H7N9) vaccine, and the induction of antibodies to hemagglutinin (HA) or neuraminidase proteins was evaluated. Ferrets were then challenged with wild-type H7N9 virus to assess the vaccine's protective efficacy. The vaccine composition and integrity was also evaluated in vitro. Results. Adjuvanted vaccines stimulated robust serum antibody titers against HA and neuraminidase compared with the unadjuvanted vaccines. Although there was a difference in adjuvanticity between AS03 and MF59 at a lower dose (3.75 μg of HA), both adjuvants induced comparable antibody responses after 2 doses of 15 μg. On challenge, ferrets that received adjuvanted vaccines showed lower viral burden than the control or unadjuvanted vaccine group. In vitro examinations revealed that the vaccine contained visible split-virus particles and retained the native conformation of HA recognizable by polyclonal and monoclonal antibodies. Conclusions. The adjuvanted H7N9 vaccines demonstrated superior immunogenicity and protective efficacy against H7N9 infection in ferrets and hold potential as a vaccination regimen.-
dc.languageeng-
dc.relation.ispartofJournal of Infectious Diseases-
dc.subjectadjuvants-
dc.subjectferrets-
dc.subjectH7N9-
dc.subjectinfluenza-
dc.subjectvaccine-
dc.titleImpact of Adjuvants on the Immunogenicity and Efficacy of Split-Virion H7N9 Vaccine in Ferrets-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/infdis/jiv099-
dc.identifier.pmid25712975-
dc.identifier.scopuseid_2-s2.0-84939646075-
dc.identifier.volume212-
dc.identifier.issue4-
dc.identifier.spage542-
dc.identifier.epage551-
dc.identifier.eissn1537-6613-
dc.identifier.isiWOS:000359677600006-

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