File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Molecular basis of mammalian transmissibility of avian H1N1 influenza viruses and their pandemic potential

TitleMolecular basis of mammalian transmissibility of avian H1N1 influenza viruses and their pandemic potential
Authors
KeywordsAirborne transmission
Avian influenza virus
Ferret
H1N1
Nonstructural protein 1
Issue Date2017
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2017, v. 114, n. 42, p. 11217-11222 How to Cite?
AbstractNorth American wild birds are an important reservoir of influenza A viruses, yet the potential of viruses in this reservoir to transmit and cause disease in mammals is not well understood. Our surveillance of avian influenza viruses (AIVs) at Delaware Bay, USA, revealed a group of similar H1N1 AIVs isolated in 2009, some of which were airborne-transmissible in the ferret model without prior adaptation. Comparison of the genomes of these viruses revealed genetic markers of airborne transmissibility in the Polymerase Basic 2 (PB2), PB1, PB1-F2, Polymerase Acidic-X (PA-X), Nonstructural Protein 1 (NS1), and Nuclear Export Protein (NEP) genes. We studied the role of NS1 in airborne transmission and found that NS1 mutants that were not airborne-transmissible caused limited tissue pathology in the upper respiratory tract (URT). Viral maturation was also delayed, evident as strong intranuclear staining and little virus at the mucosa. Our study of this naturally occurring constellation of genetic markers has provided insights into the poorly understood phenomenon of AIV airborne transmissibility by revealing a role for NS1 and characteristics of viral replication in the URT that were associated with airborne transmission. The transmissibility of these viruses further highlights the pandemic potential of AIVs in the wild bird reservoir and the need to maintain surveillance.
Persistent Identifierhttp://hdl.handle.net/10722/312027
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZanin, Mark-
dc.contributor.authorWong, Sook San-
dc.contributor.authorBarman, Subrata-
dc.contributor.authorKaewborisuth, Challika-
dc.contributor.authorVogel, Peter-
dc.contributor.authorRubrum, Adam-
dc.contributor.authorDarnell, Daniel-
dc.contributor.authorMarinova-Petkova, Atanaska-
dc.contributor.authorKrauss, Scott-
dc.contributor.authorWebby, Richard J.-
dc.contributor.authorWebster, Robert G.-
dc.date.accessioned2022-04-06T04:32:01Z-
dc.date.available2022-04-06T04:32:01Z-
dc.date.issued2017-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2017, v. 114, n. 42, p. 11217-11222-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/312027-
dc.description.abstractNorth American wild birds are an important reservoir of influenza A viruses, yet the potential of viruses in this reservoir to transmit and cause disease in mammals is not well understood. Our surveillance of avian influenza viruses (AIVs) at Delaware Bay, USA, revealed a group of similar H1N1 AIVs isolated in 2009, some of which were airborne-transmissible in the ferret model without prior adaptation. Comparison of the genomes of these viruses revealed genetic markers of airborne transmissibility in the Polymerase Basic 2 (PB2), PB1, PB1-F2, Polymerase Acidic-X (PA-X), Nonstructural Protein 1 (NS1), and Nuclear Export Protein (NEP) genes. We studied the role of NS1 in airborne transmission and found that NS1 mutants that were not airborne-transmissible caused limited tissue pathology in the upper respiratory tract (URT). Viral maturation was also delayed, evident as strong intranuclear staining and little virus at the mucosa. Our study of this naturally occurring constellation of genetic markers has provided insights into the poorly understood phenomenon of AIV airborne transmissibility by revealing a role for NS1 and characteristics of viral replication in the URT that were associated with airborne transmission. The transmissibility of these viruses further highlights the pandemic potential of AIVs in the wild bird reservoir and the need to maintain surveillance.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.subjectAirborne transmission-
dc.subjectAvian influenza virus-
dc.subjectFerret-
dc.subjectH1N1-
dc.subjectNonstructural protein 1-
dc.titleMolecular basis of mammalian transmissibility of avian H1N1 influenza viruses and their pandemic potential-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1073/pnas.1713974114-
dc.identifier.pmid28874549-
dc.identifier.scopuseid_2-s2.0-85031428371-
dc.identifier.volume114-
dc.identifier.issue42-
dc.identifier.spage11217-
dc.identifier.epage11222-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000413237900062-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats