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- Publisher Website: 10.1016/j.aquatox.2007.09.014
- Scopus: eid_2-s2.0-38149049166
- PMID: 18022256
- WOS: WOS:000253345800003
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Article: Non invasive high resolution in vivo imaging of α-naphthylisothiocyanate (ANIT) induced hepatobiliary toxicity in STII medaka
Title | Non invasive high resolution in vivo imaging of α-naphthylisothiocyanate (ANIT) induced hepatobiliary toxicity in STII medaka |
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Authors | |
Keywords | α-Naphthylisothiocyanate ANIT Biliary Biliary toxicity Fish Hepatobiliary Hepatotoxicity Liver Medaka Piscine liver Toxicity Toxicology |
Issue Date | 2008 |
Citation | Aquatic Toxicology, 2008, v. 86, n. 1, p. 20-37 How to Cite? |
Abstract | A novel transparent stock of medaka (Oryzias latipes; STII), homozygous recessive for all four pigments (iridophores, xanthophores, leucophores, melanophores), permits transcutaneous, high resolution (<1 μm) imaging of internal organs and tissues in living individuals. We applied this model to in vivo investigation of α -naphthylisothiocyanate (ANIT) induced hepatobiliary toxicity. Distinct phenotypic responses to ANIT involving all aspects of intrahepatic biliary passageways (IHBPs), particularly bile preductular epithelial cells (BPDECs), associated with transitional passageways between canaliculi and bile ductules, were observed. Alterations included: attenuation/dilation of bile canaliculi, bile preductular lesions, hydropic vacuolation of hepatocytes and BPDECs, mild BPDEC hypertrophy, and biliary epithelial cell (BEC) hyperplasia. Ex vivo histological, immunohistochemical, and ultrastructural studies were employed to aid in interpretation of, and verify, in vivo findings. 3D reconstructions from in vivo investigations provided quantitative morphometric and volumetric evaluation of ANIT exposed and untreated livers. The findings presented show for the first time in vivo evaluation of toxicity in the STII medaka hepatobiliary system, and, in conjunction with prior in vivo work characterizing normalcy, advance our comparative understanding of this lower vertebrate hepatobiliary system and its response to toxic insult. © 2007 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/313023 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.099 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Hardman, Ron | - |
dc.contributor.author | Kullman, Seth | - |
dc.contributor.author | Yuen, Bonny | - |
dc.contributor.author | Hinton, David E. | - |
dc.date.accessioned | 2022-05-26T07:00:07Z | - |
dc.date.available | 2022-05-26T07:00:07Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Aquatic Toxicology, 2008, v. 86, n. 1, p. 20-37 | - |
dc.identifier.issn | 0166-445X | - |
dc.identifier.uri | http://hdl.handle.net/10722/313023 | - |
dc.description.abstract | A novel transparent stock of medaka (Oryzias latipes; STII), homozygous recessive for all four pigments (iridophores, xanthophores, leucophores, melanophores), permits transcutaneous, high resolution (<1 μm) imaging of internal organs and tissues in living individuals. We applied this model to in vivo investigation of α -naphthylisothiocyanate (ANIT) induced hepatobiliary toxicity. Distinct phenotypic responses to ANIT involving all aspects of intrahepatic biliary passageways (IHBPs), particularly bile preductular epithelial cells (BPDECs), associated with transitional passageways between canaliculi and bile ductules, were observed. Alterations included: attenuation/dilation of bile canaliculi, bile preductular lesions, hydropic vacuolation of hepatocytes and BPDECs, mild BPDEC hypertrophy, and biliary epithelial cell (BEC) hyperplasia. Ex vivo histological, immunohistochemical, and ultrastructural studies were employed to aid in interpretation of, and verify, in vivo findings. 3D reconstructions from in vivo investigations provided quantitative morphometric and volumetric evaluation of ANIT exposed and untreated livers. The findings presented show for the first time in vivo evaluation of toxicity in the STII medaka hepatobiliary system, and, in conjunction with prior in vivo work characterizing normalcy, advance our comparative understanding of this lower vertebrate hepatobiliary system and its response to toxic insult. © 2007 Elsevier B.V. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Aquatic Toxicology | - |
dc.subject | α-Naphthylisothiocyanate | - |
dc.subject | ANIT | - |
dc.subject | Biliary | - |
dc.subject | Biliary toxicity | - |
dc.subject | Fish | - |
dc.subject | Hepatobiliary | - |
dc.subject | Hepatotoxicity | - |
dc.subject | Liver | - |
dc.subject | Medaka | - |
dc.subject | Piscine liver | - |
dc.subject | Toxicity | - |
dc.subject | Toxicology | - |
dc.title | Non invasive high resolution in vivo imaging of α-naphthylisothiocyanate (ANIT) induced hepatobiliary toxicity in STII medaka | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.aquatox.2007.09.014 | - |
dc.identifier.pmid | 18022256 | - |
dc.identifier.pmcid | PMC2724681 | - |
dc.identifier.scopus | eid_2-s2.0-38149049166 | - |
dc.identifier.volume | 86 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 20 | - |
dc.identifier.epage | 37 | - |
dc.identifier.isi | WOS:000253345800003 | - |