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Article: SARS-CoV-2 accessory proteins reveal distinct serological signatures in children

TitleSARS-CoV-2 accessory proteins reveal distinct serological signatures in children
Authors
Issue Date2022
Citation
Nature Communications, 2022, v. 13 n. 1 How to Cite?
AbstractThe antibody response magnitude and kinetics may impact clinical severity, serological diagnosis and long-term protection of COVID-19, which may play a role in why children experience lower morbidity. We therefore tested samples from 122 children in Hong Kong with symptomatic (n = 78) and asymptomatic (n = 44) SARS-CoV-2 infections up to 200 days post infection, relative to 71 infected adults (symptomatic n = 61, and asymptomatic n = 10), and negative controls (n = 48). We assessed serum IgG antibodies to a 14-wide antigen panel of structural and accessory proteins by Luciferase Immuno-Precipitation System (LIPS) assay and circulating cytokines. Infected children have lower levels of Spike, Membrane, ORF3a, ORF7a, ORF7b antibodies, comparable ORF8 and elevated E-specific antibodies than adults. Combination of two unique antibody targets, ORF3d and ORF8, can accurately discriminate SARS-CoV-2 infection in children. Principal component analysis reveals distinct pediatric serological signatures, and the highest contribution to variance from adults are antibody responses to non-structural proteins ORF3d, NSP1, ORF3a and ORF8. From a diverse panel of cytokines that can modulate immune priming and relative inflammation, IL-8, MCP-1 and IL-6 correlate with the magnitude of pediatric antibody specificity and severity. Antibodies to SARS-CoV-2 internal proteins may become an important sero surveillance tool of infection with the roll-out of vaccines in the pediatric population.
Persistent Identifierhttp://hdl.handle.net/10722/314116

 

DC FieldValueLanguage
dc.contributor.authorHachim, A-
dc.contributor.authorGu, H-
dc.contributor.authorKavian-Tessler, NC-
dc.contributor.authorMori, MASASHI-
dc.contributor.authorKwan, MIKE Y. W.-
dc.contributor.authorChan, WAI HUNG-
dc.contributor.authorYau, YAT SUN-
dc.contributor.authorChiu, SSS-
dc.contributor.authorTsang, OWEN T. Y.-
dc.contributor.authorHui, DAVID S. C-
dc.contributor.authorMok, CHRIS K. P-
dc.contributor.authorMa, NL-
dc.contributor.authorLau, EHY-
dc.contributor.authorAmarasinghe, GAYA K.-
dc.contributor.authorQavi, ABRAHAM J.-
dc.contributor.authorCheng, MS-
dc.contributor.authorPoon, LML-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorDoak, SAV-
dc.contributor.authorKavian-Tessler, NC-
dc.date.accessioned2022-07-18T06:12:01Z-
dc.date.available2022-07-18T06:12:01Z-
dc.date.issued2022-
dc.identifier.citationNature Communications, 2022, v. 13 n. 1-
dc.identifier.urihttp://hdl.handle.net/10722/314116-
dc.description.abstractThe antibody response magnitude and kinetics may impact clinical severity, serological diagnosis and long-term protection of COVID-19, which may play a role in why children experience lower morbidity. We therefore tested samples from 122 children in Hong Kong with symptomatic (n = 78) and asymptomatic (n = 44) SARS-CoV-2 infections up to 200 days post infection, relative to 71 infected adults (symptomatic n = 61, and asymptomatic n = 10), and negative controls (n = 48). We assessed serum IgG antibodies to a 14-wide antigen panel of structural and accessory proteins by Luciferase Immuno-Precipitation System (LIPS) assay and circulating cytokines. Infected children have lower levels of Spike, Membrane, ORF3a, ORF7a, ORF7b antibodies, comparable ORF8 and elevated E-specific antibodies than adults. Combination of two unique antibody targets, ORF3d and ORF8, can accurately discriminate SARS-CoV-2 infection in children. Principal component analysis reveals distinct pediatric serological signatures, and the highest contribution to variance from adults are antibody responses to non-structural proteins ORF3d, NSP1, ORF3a and ORF8. From a diverse panel of cytokines that can modulate immune priming and relative inflammation, IL-8, MCP-1 and IL-6 correlate with the magnitude of pediatric antibody specificity and severity. Antibodies to SARS-CoV-2 internal proteins may become an important sero surveillance tool of infection with the roll-out of vaccines in the pediatric population.-
dc.languageeng-
dc.relation.ispartofNature Communications-
dc.titleSARS-CoV-2 accessory proteins reveal distinct serological signatures in children-
dc.typeArticle-
dc.identifier.emailGu, H: guhaogao@hku.hk-
dc.identifier.emailChiu, SSS: ssschiu@HKUCC-COM.hku.hk-
dc.identifier.emailLau, EHY: ehylau@hku.hk-
dc.identifier.emailCheng, MS: samuelms@hku.hk-
dc.identifier.emailPoon, LML: llmpoon@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.authorityChiu, SSS=rp00421-
dc.identifier.authorityLau, EHY=rp01349-
dc.identifier.authorityPoon, LML=rp00484-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.identifier.authorityDoak, SAV=rp02141-
dc.identifier.doi10.1038/s41467-022-30699-5-
dc.identifier.hkuros334279-
dc.identifier.volume13-
dc.identifier.issue1-

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