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- Publisher Website: 10.1038/s41467-022-30699-5
- WOS: WOS:000800650200016
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Article: SARS-CoV-2 accessory proteins reveal distinct serological signatures in children
Title | SARS-CoV-2 accessory proteins reveal distinct serological signatures in children |
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Authors | |
Issue Date | 2022 |
Citation | Nature Communications, 2022, v. 13 n. 1 How to Cite? |
Abstract | The antibody response magnitude and kinetics may impact clinical severity, serological diagnosis and long-term protection of COVID-19, which may play a role in why children experience lower morbidity. We therefore tested samples from 122 children in Hong Kong with symptomatic (n = 78) and asymptomatic (n = 44) SARS-CoV-2 infections up to 200 days post infection, relative to 71 infected adults (symptomatic n = 61, and asymptomatic n = 10), and negative controls (n = 48). We assessed serum IgG antibodies to a 14-wide antigen panel of structural and accessory proteins by Luciferase Immuno-Precipitation System (LIPS) assay and circulating cytokines. Infected children have lower levels of Spike, Membrane, ORF3a, ORF7a, ORF7b antibodies, comparable ORF8 and elevated E-specific antibodies than adults. Combination of two unique antibody targets, ORF3d and ORF8, can accurately discriminate SARS-CoV-2 infection in children. Principal component analysis reveals distinct pediatric serological signatures, and the highest contribution to variance from adults are antibody responses to non-structural proteins ORF3d, NSP1, ORF3a and ORF8. From a diverse panel of cytokines that can modulate immune priming and relative inflammation, IL-8, MCP-1 and IL-6 correlate with the magnitude of pediatric antibody specificity and severity. Antibodies to SARS-CoV-2 internal proteins may become an important sero surveillance tool of infection with the roll-out of vaccines in the pediatric population. |
Persistent Identifier | http://hdl.handle.net/10722/314116 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Hachim, A | - |
dc.contributor.author | Gu, H | - |
dc.contributor.author | Kavian-Tessler, NC | - |
dc.contributor.author | Mori, MASASHI | - |
dc.contributor.author | Kwan, MIKE Y. W. | - |
dc.contributor.author | Chan, WAI HUNG | - |
dc.contributor.author | Yau, YAT SUN | - |
dc.contributor.author | Chiu, SSS | - |
dc.contributor.author | Tsang, OWEN T. Y. | - |
dc.contributor.author | Hui, DAVID S. C | - |
dc.contributor.author | Mok, CHRIS K. P | - |
dc.contributor.author | Ma, NL | - |
dc.contributor.author | Lau, EHY | - |
dc.contributor.author | Amarasinghe, GAYA K. | - |
dc.contributor.author | Qavi, ABRAHAM J. | - |
dc.contributor.author | Cheng, MS | - |
dc.contributor.author | Poon, LML | - |
dc.contributor.author | Peiris, JSM | - |
dc.contributor.author | Doak, SAV | - |
dc.contributor.author | Kavian-Tessler, NC | - |
dc.date.accessioned | 2022-07-18T06:12:01Z | - |
dc.date.available | 2022-07-18T06:12:01Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Nature Communications, 2022, v. 13 n. 1 | - |
dc.identifier.uri | http://hdl.handle.net/10722/314116 | - |
dc.description.abstract | The antibody response magnitude and kinetics may impact clinical severity, serological diagnosis and long-term protection of COVID-19, which may play a role in why children experience lower morbidity. We therefore tested samples from 122 children in Hong Kong with symptomatic (n = 78) and asymptomatic (n = 44) SARS-CoV-2 infections up to 200 days post infection, relative to 71 infected adults (symptomatic n = 61, and asymptomatic n = 10), and negative controls (n = 48). We assessed serum IgG antibodies to a 14-wide antigen panel of structural and accessory proteins by Luciferase Immuno-Precipitation System (LIPS) assay and circulating cytokines. Infected children have lower levels of Spike, Membrane, ORF3a, ORF7a, ORF7b antibodies, comparable ORF8 and elevated E-specific antibodies than adults. Combination of two unique antibody targets, ORF3d and ORF8, can accurately discriminate SARS-CoV-2 infection in children. Principal component analysis reveals distinct pediatric serological signatures, and the highest contribution to variance from adults are antibody responses to non-structural proteins ORF3d, NSP1, ORF3a and ORF8. From a diverse panel of cytokines that can modulate immune priming and relative inflammation, IL-8, MCP-1 and IL-6 correlate with the magnitude of pediatric antibody specificity and severity. Antibodies to SARS-CoV-2 internal proteins may become an important sero surveillance tool of infection with the roll-out of vaccines in the pediatric population. | - |
dc.language | eng | - |
dc.relation.ispartof | Nature Communications | - |
dc.title | SARS-CoV-2 accessory proteins reveal distinct serological signatures in children | - |
dc.type | Article | - |
dc.identifier.email | Gu, H: guhaogao@hku.hk | - |
dc.identifier.email | Chiu, SSS: ssschiu@HKUCC-COM.hku.hk | - |
dc.identifier.email | Lau, EHY: ehylau@hku.hk | - |
dc.identifier.email | Cheng, MS: samuelms@hku.hk | - |
dc.identifier.email | Poon, LML: llmpoon@hkucc.hku.hk | - |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | - |
dc.identifier.authority | Chiu, SSS=rp00421 | - |
dc.identifier.authority | Lau, EHY=rp01349 | - |
dc.identifier.authority | Poon, LML=rp00484 | - |
dc.identifier.authority | Peiris, JSM=rp00410 | - |
dc.identifier.authority | Doak, SAV=rp02141 | - |
dc.identifier.doi | 10.1038/s41467-022-30699-5 | - |
dc.identifier.hkuros | 334279 | - |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 1 | - |
dc.identifier.isi | WOS:000800650200016 | - |