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- Publisher Website: 10.1038/s41467-021-24887-y
- Scopus: eid_2-s2.0-85111625783
- PMID: 34330917
- WOS: WOS:000684300300001
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Article: Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress
Title | Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress |
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Authors | Mendonça, LuizaHowe, AndrewGilchrist, James B.Sheng, YuewenSun, DapengKnight, Michael L.Zanetti-Domingues, Laura C.Bateman, BenjiKrebs, Anna SophiaChen, LongRadecke, JulikaLi, Vivian D.Ni, TaoKounatidis, IliasKoronfel, Mohamed A.Szynkiewicz, MartaHarkiolaki, MariaMartin-Fernandez, Marisa L.James, WilliamZhang, Peijun |
Issue Date | 2021 |
Citation | Nature Communications, 2021, v. 12, n. 1, article no. 4629 How to Cite? |
Abstract | Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events – e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. |
Persistent Identifier | http://hdl.handle.net/10722/316594 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Mendonça, Luiza | - |
dc.contributor.author | Howe, Andrew | - |
dc.contributor.author | Gilchrist, James B. | - |
dc.contributor.author | Sheng, Yuewen | - |
dc.contributor.author | Sun, Dapeng | - |
dc.contributor.author | Knight, Michael L. | - |
dc.contributor.author | Zanetti-Domingues, Laura C. | - |
dc.contributor.author | Bateman, Benji | - |
dc.contributor.author | Krebs, Anna Sophia | - |
dc.contributor.author | Chen, Long | - |
dc.contributor.author | Radecke, Julika | - |
dc.contributor.author | Li, Vivian D. | - |
dc.contributor.author | Ni, Tao | - |
dc.contributor.author | Kounatidis, Ilias | - |
dc.contributor.author | Koronfel, Mohamed A. | - |
dc.contributor.author | Szynkiewicz, Marta | - |
dc.contributor.author | Harkiolaki, Maria | - |
dc.contributor.author | Martin-Fernandez, Marisa L. | - |
dc.contributor.author | James, William | - |
dc.contributor.author | Zhang, Peijun | - |
dc.date.accessioned | 2022-09-14T11:40:50Z | - |
dc.date.available | 2022-09-14T11:40:50Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Nature Communications, 2021, v. 12, n. 1, article no. 4629 | - |
dc.identifier.uri | http://hdl.handle.net/10722/316594 | - |
dc.description.abstract | Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events – e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. | - |
dc.language | eng | - |
dc.relation.ispartof | Nature Communications | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1038/s41467-021-24887-y | - |
dc.identifier.pmid | 34330917 | - |
dc.identifier.pmcid | PMC8324836 | - |
dc.identifier.scopus | eid_2-s2.0-85111625783 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 4629 | - |
dc.identifier.epage | article no. 4629 | - |
dc.identifier.eissn | 2041-1723 | - |
dc.identifier.isi | WOS:000684300300001 | - |