File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1126/sciadv.abj5715
- Scopus: eid_2-s2.0-85119965631
- PMID: 34797722
- WOS: WOS:000722925000012
Supplementary
- Citations:
- Appears in Collections:
Article: Structure of native HIV-1 cores and their interactions with IP6 and CypA
Title | Structure of native HIV-1 cores and their interactions with IP6 and CypA |
---|---|
Authors | |
Issue Date | 2021 |
Citation | Science Advances, 2021, v. 7, n. 47, article no. abj5715 How to Cite? |
Abstract | The viral capsid plays essential roles in HIV replication and is a major platform engaging host factors. To overcome challenges in study native capsid structure, we used the perfringolysin O to perforate the membrane of HIV-1 particles, thus allowing host proteins and small molecules to access the native capsid while improving cryo-electron microscopy image quality. Using cryo-electron tomography and subtomogram averaging, we determined the structures of native capsomers in the presence and absence of inositol hexakisphosphate (IP6) and cyclophilin A and constructed an all-atom model of a complete HIV-1 capsid. Our structures reveal two IP6 binding sites and modes of cyclophilin A interactions. Free energy calculations substantiate the two binding sites at R18 and K25 and further show a prohibitive energy barrier for IP6 to pass through the pentamer. Our results demonstrate that perfringolysin O perforation is a valuable tool for structural analyses of enveloped virus capsids and interactions with host cell factors. |
Persistent Identifier | http://hdl.handle.net/10722/316637 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ni, Tao | - |
dc.contributor.author | Zhu, Yanan | - |
dc.contributor.author | Yang, Zhengyi | - |
dc.contributor.author | Xu, Chaoyi | - |
dc.contributor.author | Chaban, Yuriy | - |
dc.contributor.author | Nesterova, Tanya | - |
dc.contributor.author | Ning, Jiying | - |
dc.contributor.author | Böcking, Till | - |
dc.contributor.author | Parker, Michael W. | - |
dc.contributor.author | Monnie, Christina | - |
dc.contributor.author | Ahn, Jinwoo | - |
dc.contributor.author | Perilla, Juan R. | - |
dc.contributor.author | Zhang, Peijun | - |
dc.date.accessioned | 2022-09-14T11:40:56Z | - |
dc.date.available | 2022-09-14T11:40:56Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Science Advances, 2021, v. 7, n. 47, article no. abj5715 | - |
dc.identifier.uri | http://hdl.handle.net/10722/316637 | - |
dc.description.abstract | The viral capsid plays essential roles in HIV replication and is a major platform engaging host factors. To overcome challenges in study native capsid structure, we used the perfringolysin O to perforate the membrane of HIV-1 particles, thus allowing host proteins and small molecules to access the native capsid while improving cryo-electron microscopy image quality. Using cryo-electron tomography and subtomogram averaging, we determined the structures of native capsomers in the presence and absence of inositol hexakisphosphate (IP6) and cyclophilin A and constructed an all-atom model of a complete HIV-1 capsid. Our structures reveal two IP6 binding sites and modes of cyclophilin A interactions. Free energy calculations substantiate the two binding sites at R18 and K25 and further show a prohibitive energy barrier for IP6 to pass through the pentamer. Our results demonstrate that perfringolysin O perforation is a valuable tool for structural analyses of enveloped virus capsids and interactions with host cell factors. | - |
dc.language | eng | - |
dc.relation.ispartof | Science Advances | - |
dc.title | Structure of native HIV-1 cores and their interactions with IP6 and CypA | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1126/sciadv.abj5715 | - |
dc.identifier.pmid | 34797722 | - |
dc.identifier.scopus | eid_2-s2.0-85119965631 | - |
dc.identifier.volume | 7 | - |
dc.identifier.issue | 47 | - |
dc.identifier.spage | article no. abj5715 | - |
dc.identifier.epage | article no. abj5715 | - |
dc.identifier.eissn | 2375-2548 | - |
dc.identifier.isi | WOS:000722925000012 | - |