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Article: Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology

TitleGeneration of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology
Authors
SUN, YFU, JYANG, JZhao, JRong, J
Issue Date2022
Citation
Stem Cell Research, 2022, v. 63 How to Cite?
DOI: http://dx.doi.org/10.1016/j.scr.2022.102859
AbstractRas-related GTP-binding protein A (RagA), encoded by RRAGA gene, initially senses the availability of cellular amino acids (e.g., leucine) and controls the translocation of mTORC1 to the lysosomal membrane. RagA overexpression appears to be associated with the onset of depression. To discover the biological roles of RagA, we employed the CRISPR/Cas9 system to generate a RRAGA gene knockout stem cell line from human induced pluripotent stem cell (iPSC) iPSN0003. Such RRAGA knockout iPSC cell line may help the development of new therapeutics for depression.
Persistent Identifierhttp://hdl.handle.net/10722/319931
ISSN
1873-5061
2023 Impact Factor: 0.8
2023 SCImago Journal Rankings: 0.467
ISI Accession Number ID
WOS:000834090000001

 

DC FieldValueLanguage
dc.contributor.authorSUN, Y-
dc.contributor.authorFU, J-
dc.contributor.authorYANG, J-
dc.contributor.authorZhao, J-
dc.contributor.authorRong, J-
dc.date.accessioned2022-10-14T05:22:22Z-
dc.date.available2022-10-14T05:22:22Z-
dc.date.issued2022-
dc.identifier.citationStem Cell Research, 2022, v. 63-
dc.identifier.issn1873-5061-
dc.identifier.urihttp://hdl.handle.net/10722/319931-
dc.description.abstractRas-related GTP-binding protein A (RagA), encoded by RRAGA gene, initially senses the availability of cellular amino acids (e.g., leucine) and controls the translocation of mTORC1 to the lysosomal membrane. RagA overexpression appears to be associated with the onset of depression. To discover the biological roles of RagA, we employed the CRISPR/Cas9 system to generate a RRAGA gene knockout stem cell line from human induced pluripotent stem cell (iPSC) iPSN0003. Such RRAGA knockout iPSC cell line may help the development of new therapeutics for depression.-
dc.languageeng-
dc.relation.ispartofStem Cell Research-
dc.titleGeneration of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology-
dc.typeArticle-
dc.identifier.emailZhao, J: zhaojia7@hku.hk-
dc.identifier.emailRong, J: jrong@hku.hk-
dc.identifier.authorityRong, J=rp00515-
dc.identifier.doi10.1016/j.scr.2022.102859-
dc.identifier.pmid35870248-
dc.identifier.scopuseid_2-s2.0-85134785306-
dc.identifier.hkuros338570-
dc.identifier.volume63-
dc.identifier.eissn1876-7753-
dc.identifier.isiWOS:000834090000001-

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