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Article: The effect of gold nanoparticle size on osteogenic differentiation of adipose-derived stem cells

TitleThe effect of gold nanoparticle size on osteogenic differentiation of adipose-derived stem cells
Authors
KeywordsGold nanoparticles
Human adipose-derived stem cell
Osteogenesis
Osteogenic differentiation
Uptake
Issue Date2015
Citation
Journal of Colloid and Interface Science, 2015, v. 438, p. 68-76 How to Cite?
AbstractThere have been many medical applications based on gold nanoparticles (GNPs) over the past several centuries. Recently, researchers have focused on bone tissue engineering applications utilizing GNPs. The effect of various sizes of gold nanoparticles on the differentiation of human adipose-derived stem cells (ADSCs) into osteoblasts was investigated. The concentration of gold nanoparticles was fixed at 1. μM and varying sizes of 15, 30, 50, 75 and 100. nm (spherical GNPs) were used. The lack of cytotoxicity was confirmed by establishing viability of ADSCs using cell counting kit-8 (CCK-8) and live/dead assays. The results showed that each size of GNPs had no significant toxicity on ADSCs during 1. week of incubation. Osteogenic differentiation of ADSCs was confirmed by alkaline phosphatase (ALP) staining, ALP activity, calcium deposition, and real time PCR experiments. It was found, through dark field assays and microscope cell images, that 30. nm and 50. nm GNPs were preferentially up taken into the ADSCs. As expected, all sizes of gold nanoparticles promoted the differentiation of ADSCs toward osteoblasts more than control. Among all sizes, 30 and 50. nm GNPs appeared to have the highest differentiation rates. The data consistently demonstrated that 30 and 50. nm GNPs are the most effective in promoting osteogenic differentiation of ADSCs.
Persistent Identifierhttp://hdl.handle.net/10722/323921
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 1.760
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKo, Wan Kyu-
dc.contributor.authorHeo, Dong Nyoung-
dc.contributor.authorMoon, Ho Jin-
dc.contributor.authorLee, Sang Jin-
dc.contributor.authorBae, Min Soo-
dc.contributor.authorLee, Jung Bok-
dc.contributor.authorSun, In Cheol-
dc.contributor.authorJeon, Hoon Bong-
dc.contributor.authorPark, Hun Kuk-
dc.contributor.authorKwon, Il Keun-
dc.date.accessioned2023-01-13T03:00:15Z-
dc.date.available2023-01-13T03:00:15Z-
dc.date.issued2015-
dc.identifier.citationJournal of Colloid and Interface Science, 2015, v. 438, p. 68-76-
dc.identifier.issn0021-9797-
dc.identifier.urihttp://hdl.handle.net/10722/323921-
dc.description.abstractThere have been many medical applications based on gold nanoparticles (GNPs) over the past several centuries. Recently, researchers have focused on bone tissue engineering applications utilizing GNPs. The effect of various sizes of gold nanoparticles on the differentiation of human adipose-derived stem cells (ADSCs) into osteoblasts was investigated. The concentration of gold nanoparticles was fixed at 1. μM and varying sizes of 15, 30, 50, 75 and 100. nm (spherical GNPs) were used. The lack of cytotoxicity was confirmed by establishing viability of ADSCs using cell counting kit-8 (CCK-8) and live/dead assays. The results showed that each size of GNPs had no significant toxicity on ADSCs during 1. week of incubation. Osteogenic differentiation of ADSCs was confirmed by alkaline phosphatase (ALP) staining, ALP activity, calcium deposition, and real time PCR experiments. It was found, through dark field assays and microscope cell images, that 30. nm and 50. nm GNPs were preferentially up taken into the ADSCs. As expected, all sizes of gold nanoparticles promoted the differentiation of ADSCs toward osteoblasts more than control. Among all sizes, 30 and 50. nm GNPs appeared to have the highest differentiation rates. The data consistently demonstrated that 30 and 50. nm GNPs are the most effective in promoting osteogenic differentiation of ADSCs.-
dc.languageeng-
dc.relation.ispartofJournal of Colloid and Interface Science-
dc.subjectGold nanoparticles-
dc.subjectHuman adipose-derived stem cell-
dc.subjectOsteogenesis-
dc.subjectOsteogenic differentiation-
dc.subjectUptake-
dc.titleThe effect of gold nanoparticle size on osteogenic differentiation of adipose-derived stem cells-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jcis.2014.08.058-
dc.identifier.pmid25454427-
dc.identifier.scopuseid_2-s2.0-84908377690-
dc.identifier.volume438-
dc.identifier.spage68-
dc.identifier.epage76-
dc.identifier.eissn1095-7103-
dc.identifier.isiWOS:000346692100010-

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