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Article: Core promoter-specific function of a mutant transcription factor TFIID defective in TATA-box binding

TitleCore promoter-specific function of a mutant transcription factor TFIID defective in TATA-box binding
Authors
Issue Date1995
Citation
Proceedings of the National Academy of Sciences of the United States of America, 1995, v. 92, n. 25, p. 11864-11868 How to Cite?
AbstractIn conjunction with other general initiation factors, the TATA box- binding protein (TBP) can direct basal transcription by RNA polymerase II from TATA-containing promoters, but its stable interaction with TBP- associated factors (TAFs) in the TFIID complex is required both for activator-dependent transcription and for basal transcription directed by an initiator element. We have generated a TATA-binding-defective TFIID complex containing an amino acid substitution in the DNA-binding surface of its TBP subunit. This mutated TFIID is defective in both basal and activated transcription from core promoters containing only a TATA box but supports transcription from initiator-containing promoters independently of the presence or absence of a TATA sequence. Our results show that a functional initiator element is needed to bypass the requirement for an active TATA DNA- binding surface in TFIID and imply that gene-specific transcription can be achieved by modulating distinct core promoter-specific TFIID functions-e.g., TBP-TATA versus TAF-initiator interactions.
Persistent Identifierhttp://hdl.handle.net/10722/324992
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMartinez, Ernest-
dc.contributor.authorZhou, Qiang-
dc.contributor.authorL'Etoile, Noelle D.-
dc.contributor.authorOelgeschläger, Thomas-
dc.contributor.authorBerk, Arnold J.-
dc.contributor.authorRoeder, Robert G.-
dc.date.accessioned2023-02-27T07:28:49Z-
dc.date.available2023-02-27T07:28:49Z-
dc.date.issued1995-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 1995, v. 92, n. 25, p. 11864-11868-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/324992-
dc.description.abstractIn conjunction with other general initiation factors, the TATA box- binding protein (TBP) can direct basal transcription by RNA polymerase II from TATA-containing promoters, but its stable interaction with TBP- associated factors (TAFs) in the TFIID complex is required both for activator-dependent transcription and for basal transcription directed by an initiator element. We have generated a TATA-binding-defective TFIID complex containing an amino acid substitution in the DNA-binding surface of its TBP subunit. This mutated TFIID is defective in both basal and activated transcription from core promoters containing only a TATA box but supports transcription from initiator-containing promoters independently of the presence or absence of a TATA sequence. Our results show that a functional initiator element is needed to bypass the requirement for an active TATA DNA- binding surface in TFIID and imply that gene-specific transcription can be achieved by modulating distinct core promoter-specific TFIID functions-e.g., TBP-TATA versus TAF-initiator interactions.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.titleCore promoter-specific function of a mutant transcription factor TFIID defective in TATA-box binding-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1073/pnas.92.25.11864-
dc.identifier.pmid8524864-
dc.identifier.scopuseid_2-s2.0-0029610077-
dc.identifier.volume92-
dc.identifier.issue25-
dc.identifier.spage11864-
dc.identifier.epage11868-
dc.identifier.isiWOS:A1995TJ22200112-

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