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Article: The Dynamics of Microglial Polarization Reveal the Resident Neuroinflammatory Responses After Subarachnoid Hemorrhage

TitleThe Dynamics of Microglial Polarization Reveal the Resident Neuroinflammatory Responses After Subarachnoid Hemorrhage
Authors
KeywordsMicroglia
Neuroinflammation
Polarization
Stroke
Subarachnoid hemorrhage
Issue Date2020
Citation
Translational Stroke Research, 2020, v. 11, n. 3, p. 433-449 How to Cite?
AbstractNeuroinflammation plays a critical role in the pathogenesis of subarachnoid hemorrhage (SAH). Microglia, as the resident immune cells, orchestrate neuroinflammation distinctly in neurological diseases with different polarization statuses. However, microglial polarizations in the neuroinflammatory responses after SAH are not fully understood. In this study, we investigated the dynamics of microglial reaction in an endovascular perforated SAH model. By using the Cx3cr1GFP/GFP Ccr2RFP/RFP transgenic mice, we found that the reactive immune cells were largely from resident microglia pool rather than infiltrating macrophages. Immunostaining and real-time PCR were employed to analyze the temporal microglial polarization and the resulting inflammatory responses. Our results showed that microglia accumulated immediately after SAH with a centrifugal spreading through the Cortex Adjacent to the Perforated Site (CAPS) to the remote motor cortex. Microglia polarized dynamically from M1 to M2 phenotype along with the morphological transformation from ramified to amoeboid shapes. The ramified microglia demonstrated the M1 property, which suggested the function-related microglial polarization occurred prior to morphological transformation after SAH. Bipolar-shaped microglia appeared as the intermediate and transitional status with the capacity of bidirectional polarization. The microglial polarization status is distinct in molecular inflammatory responses; M1-related pro-inflammation was predominant in the early phase and subsequently transited to the M2-related anti-inflammation. The systematic characterization of the dynamics of microglial polarization in this study contributes to the understanding of the origin of neuroinflammatory responses after SAH and provides key foundation for further investigations to develop target treatment.
Persistent Identifierhttp://hdl.handle.net/10722/325454
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 1.595
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZheng, Zhiyuan Vera-
dc.contributor.authorLyu, Hao-
dc.contributor.authorLam, Sin Yu Erica-
dc.contributor.authorLam, Ping Kuen-
dc.contributor.authorPoon, Wai Sang-
dc.contributor.authorWong, George Kwok Chu-
dc.date.accessioned2023-02-27T07:33:27Z-
dc.date.available2023-02-27T07:33:27Z-
dc.date.issued2020-
dc.identifier.citationTranslational Stroke Research, 2020, v. 11, n. 3, p. 433-449-
dc.identifier.issn1868-4483-
dc.identifier.urihttp://hdl.handle.net/10722/325454-
dc.description.abstractNeuroinflammation plays a critical role in the pathogenesis of subarachnoid hemorrhage (SAH). Microglia, as the resident immune cells, orchestrate neuroinflammation distinctly in neurological diseases with different polarization statuses. However, microglial polarizations in the neuroinflammatory responses after SAH are not fully understood. In this study, we investigated the dynamics of microglial reaction in an endovascular perforated SAH model. By using the Cx3cr1GFP/GFP Ccr2RFP/RFP transgenic mice, we found that the reactive immune cells were largely from resident microglia pool rather than infiltrating macrophages. Immunostaining and real-time PCR were employed to analyze the temporal microglial polarization and the resulting inflammatory responses. Our results showed that microglia accumulated immediately after SAH with a centrifugal spreading through the Cortex Adjacent to the Perforated Site (CAPS) to the remote motor cortex. Microglia polarized dynamically from M1 to M2 phenotype along with the morphological transformation from ramified to amoeboid shapes. The ramified microglia demonstrated the M1 property, which suggested the function-related microglial polarization occurred prior to morphological transformation after SAH. Bipolar-shaped microglia appeared as the intermediate and transitional status with the capacity of bidirectional polarization. The microglial polarization status is distinct in molecular inflammatory responses; M1-related pro-inflammation was predominant in the early phase and subsequently transited to the M2-related anti-inflammation. The systematic characterization of the dynamics of microglial polarization in this study contributes to the understanding of the origin of neuroinflammatory responses after SAH and provides key foundation for further investigations to develop target treatment.-
dc.languageeng-
dc.relation.ispartofTranslational Stroke Research-
dc.subjectMicroglia-
dc.subjectNeuroinflammation-
dc.subjectPolarization-
dc.subjectStroke-
dc.subjectSubarachnoid hemorrhage-
dc.titleThe Dynamics of Microglial Polarization Reveal the Resident Neuroinflammatory Responses After Subarachnoid Hemorrhage-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s12975-019-00728-5-
dc.identifier.pmid31628642-
dc.identifier.scopuseid_2-s2.0-85074583989-
dc.identifier.volume11-
dc.identifier.issue3-
dc.identifier.spage433-
dc.identifier.epage449-
dc.identifier.eissn1868-601X-
dc.identifier.isiWOS:000491412000001-

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