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Article: Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain

TitleFactors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain
Authors
KeywordsRNA polymerase II
TBP-associated factors
TFIID
Transcription factor
Transcriptional activation
Issue Date1993
Citation
Genes and Development, 1993, v. 7, n. 2, p. 180-187 How to Cite?
AbstractTFIID is a multisubunit protein containing the TATA box-binding polypeptide (TBP) and associated factors (TFIID-TAFs) required for activated transcription by RNA polymerase II. TBPs from different eukaryotes contain a highly conserved carboxy-terminal domain and very divergent amino-terminal domains. Earlier studies proposed that the amino-terminal domains of metazoan TBPs are required for activated transcription. However, we report that a human TFIID complex containing an amino-terminal truncated TBP contains all the major TFIID-TAFs and supports in vitro transcriptional stimulation by different classes of activation domains and from a TATA-less promoter. Protein blotting experiments revealed direct interactions between the conserved domain of TBP and the two largest TAFs. The results suggest a model for the interaction of TFIID-TAFs with TBP.
Persistent Identifierhttp://hdl.handle.net/10722/325603
ISSN
2023 Impact Factor: 7.5
2023 SCImago Journal Rankings: 5.015
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhou, Qiang-
dc.contributor.authorBoyer, Thomas G.-
dc.contributor.authorBerk, Arnold J.-
dc.date.accessioned2023-02-27T07:34:41Z-
dc.date.available2023-02-27T07:34:41Z-
dc.date.issued1993-
dc.identifier.citationGenes and Development, 1993, v. 7, n. 2, p. 180-187-
dc.identifier.issn0890-9369-
dc.identifier.urihttp://hdl.handle.net/10722/325603-
dc.description.abstractTFIID is a multisubunit protein containing the TATA box-binding polypeptide (TBP) and associated factors (TFIID-TAFs) required for activated transcription by RNA polymerase II. TBPs from different eukaryotes contain a highly conserved carboxy-terminal domain and very divergent amino-terminal domains. Earlier studies proposed that the amino-terminal domains of metazoan TBPs are required for activated transcription. However, we report that a human TFIID complex containing an amino-terminal truncated TBP contains all the major TFIID-TAFs and supports in vitro transcriptional stimulation by different classes of activation domains and from a TATA-less promoter. Protein blotting experiments revealed direct interactions between the conserved domain of TBP and the two largest TAFs. The results suggest a model for the interaction of TFIID-TAFs with TBP.-
dc.languageeng-
dc.relation.ispartofGenes and Development-
dc.subjectRNA polymerase II-
dc.subjectTBP-associated factors-
dc.subjectTFIID-
dc.subjectTranscription factor-
dc.subjectTranscriptional activation-
dc.titleFactors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1101/gad.7.2.180-
dc.identifier.pmid8436290-
dc.identifier.scopuseid_2-s2.0-0027526424-
dc.identifier.volume7-
dc.identifier.issue2-
dc.identifier.spage180-
dc.identifier.epage187-
dc.identifier.isiWOS:A1993KM85900002-

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