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Article: MiR-383 is downregulated in medulloblastoma and targets peroxiredoxin 3 (PRDX3)

TitleMiR-383 is downregulated in medulloblastoma and targets peroxiredoxin 3 (PRDX3)
Authors
Keywordscell growth
medulloblastoma
microRNA
PRDX3
Issue Date2013
Citation
Brain Pathology, 2013, v. 23, n. 4, p. 413-425 How to Cite?
AbstractAccumulating evidence suggests that microRNAs (miRNAs) are over- or under-expressed in tumors, and abnormalities in miRNA expression may contribute to carcinogenesis. MiR-383 was previously identified as one of the under-expresssed miRNAs in medulloblastoma (MB) by miRNA expression profiling. Quantitative reverse transcription polymerase chain reaction (RT-PCR)-based miRNA assays showed an enrichment of miR-383 in normal brain. Based on these data, we speculated that miR-383 is important in MB pathogenesis. In this study, we demonstrated significant downregulation of miR-383 in 23/29 (79%) MB samples and 7/7 (100%) MB cells lines. Ectopic expression of miR-383 in MB cells led to suppression of cell growth, cell accumulation at sub-G1 phase and alteration of apoptosis-related proteins. By transcriptomic analysis and computational algorithms, we identified peroxiredoxin 3 (PRDX3) as a target gene of miR-383. Luciferase reporter assay confirmed that miR-383 negatively regulated PRDX3 by interaction between miR-383 and complementary sequences in the 3′ UTR of PRDX3. MiR-383 repressed PRDX3 at transcriptional and translational levels as revealed by quantitative RT-PCR and Western blot analysis. Furthermore, depletion of PRDX3 by siRNAs resulted in similar effects as observed in miR-383-transfected cells. In conclusion, miR-383 acts as a regulator controlling cell growth of MB, at least in part, through targeting PRDX3. © 2012 International Society of Neuropathology.
Persistent Identifierhttp://hdl.handle.net/10722/325665
ISSN
2023 Impact Factor: 5.8
2023 SCImago Journal Rankings: 1.937
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, Kay Ka Wai-
dc.contributor.authorPang, Jesse Chung Sean-
dc.contributor.authorLau, Kin Mang-
dc.contributor.authorZhou, Liangfu-
dc.contributor.authorMao, Ying-
dc.contributor.authorWang, Yin-
dc.contributor.authorPoon, Wai Sang-
dc.contributor.authorNg, Ho Keung-
dc.date.accessioned2023-02-27T07:35:14Z-
dc.date.available2023-02-27T07:35:14Z-
dc.date.issued2013-
dc.identifier.citationBrain Pathology, 2013, v. 23, n. 4, p. 413-425-
dc.identifier.issn1015-6305-
dc.identifier.urihttp://hdl.handle.net/10722/325665-
dc.description.abstractAccumulating evidence suggests that microRNAs (miRNAs) are over- or under-expressed in tumors, and abnormalities in miRNA expression may contribute to carcinogenesis. MiR-383 was previously identified as one of the under-expresssed miRNAs in medulloblastoma (MB) by miRNA expression profiling. Quantitative reverse transcription polymerase chain reaction (RT-PCR)-based miRNA assays showed an enrichment of miR-383 in normal brain. Based on these data, we speculated that miR-383 is important in MB pathogenesis. In this study, we demonstrated significant downregulation of miR-383 in 23/29 (79%) MB samples and 7/7 (100%) MB cells lines. Ectopic expression of miR-383 in MB cells led to suppression of cell growth, cell accumulation at sub-G1 phase and alteration of apoptosis-related proteins. By transcriptomic analysis and computational algorithms, we identified peroxiredoxin 3 (PRDX3) as a target gene of miR-383. Luciferase reporter assay confirmed that miR-383 negatively regulated PRDX3 by interaction between miR-383 and complementary sequences in the 3′ UTR of PRDX3. MiR-383 repressed PRDX3 at transcriptional and translational levels as revealed by quantitative RT-PCR and Western blot analysis. Furthermore, depletion of PRDX3 by siRNAs resulted in similar effects as observed in miR-383-transfected cells. In conclusion, miR-383 acts as a regulator controlling cell growth of MB, at least in part, through targeting PRDX3. © 2012 International Society of Neuropathology.-
dc.languageeng-
dc.relation.ispartofBrain Pathology-
dc.subjectcell growth-
dc.subjectmedulloblastoma-
dc.subjectmicroRNA-
dc.subjectPRDX3-
dc.titleMiR-383 is downregulated in medulloblastoma and targets peroxiredoxin 3 (PRDX3)-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/bpa.12014-
dc.identifier.pmid23227829-
dc.identifier.scopuseid_2-s2.0-84879222079-
dc.identifier.volume23-
dc.identifier.issue4-
dc.identifier.spage413-
dc.identifier.epage425-
dc.identifier.eissn1750-3639-
dc.identifier.isiWOS:000320385900006-

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