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Article: High-Efficiency Cellular Reprogramming by Nanoscale Puncturing

TitleHigh-Efficiency Cellular Reprogramming by Nanoscale Puncturing
Authors
Keywordscell reprogramming
cell transfection
diamond nanoneedle
induced pluripotency
intracellular delivery
membrane disruption
Issue Date2020
Citation
Nano Letters, 2020, v. 20, n. 7, p. 5473-5481 How to Cite?
AbstractInduced pluripotent stem cells (iPSCs) bear great potential for disease modeling, drug discovery, and regenerative medicine; however, the wide adoption of iPSC for clinically relevant applications has been hindered by the extremely low reprogramming efficiency. Here, we describe a high-efficiency cellular reprogramming strategy by puncturing cells with an array of diamond nanoneedles, which is applied to temporally disrupt the cell membrane in a reversible and minimally invasive format. This method enables high-efficiency cytoplasmic delivery of mini-intronic plasmid vectors to initiate the conversion of human fibroblast cells to either primed iPSCs or nal¨ve iPSCs. The nanopuncturing operation is directly performed on cells in adherent culture without any cell lift-off and is completed within just 5 min. The treated cells are then cultured in feeder-free medium to achieve a reprogramming efficiency of 1.17 ± 0.28%, which is more than 2 orders of magnitude higher than the typical results from common methods involving plasmid delivery.
Persistent Identifierhttp://hdl.handle.net/10722/326228
ISSN
2023 Impact Factor: 9.6
2023 SCImago Journal Rankings: 3.411
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Yuan-
dc.contributor.authorWang, Zixun-
dc.contributor.authorXie, Kai-
dc.contributor.authorZhao, Xi-
dc.contributor.authorJiang, Xuezhen-
dc.contributor.authorChen, Bing-
dc.contributor.authorHan, Ying-
dc.contributor.authorLu, Yang-
dc.contributor.authorHuang, Linfeng-
dc.contributor.authorZhang, Wenjun-
dc.contributor.authorYang, Yang-
dc.contributor.authorShi, Peng-
dc.date.accessioned2023-03-09T09:59:03Z-
dc.date.available2023-03-09T09:59:03Z-
dc.date.issued2020-
dc.identifier.citationNano Letters, 2020, v. 20, n. 7, p. 5473-5481-
dc.identifier.issn1530-6984-
dc.identifier.urihttp://hdl.handle.net/10722/326228-
dc.description.abstractInduced pluripotent stem cells (iPSCs) bear great potential for disease modeling, drug discovery, and regenerative medicine; however, the wide adoption of iPSC for clinically relevant applications has been hindered by the extremely low reprogramming efficiency. Here, we describe a high-efficiency cellular reprogramming strategy by puncturing cells with an array of diamond nanoneedles, which is applied to temporally disrupt the cell membrane in a reversible and minimally invasive format. This method enables high-efficiency cytoplasmic delivery of mini-intronic plasmid vectors to initiate the conversion of human fibroblast cells to either primed iPSCs or nal¨ve iPSCs. The nanopuncturing operation is directly performed on cells in adherent culture without any cell lift-off and is completed within just 5 min. The treated cells are then cultured in feeder-free medium to achieve a reprogramming efficiency of 1.17 ± 0.28%, which is more than 2 orders of magnitude higher than the typical results from common methods involving plasmid delivery.-
dc.languageeng-
dc.relation.ispartofNano Letters-
dc.subjectcell reprogramming-
dc.subjectcell transfection-
dc.subjectdiamond nanoneedle-
dc.subjectinduced pluripotency-
dc.subjectintracellular delivery-
dc.subjectmembrane disruption-
dc.titleHigh-Efficiency Cellular Reprogramming by Nanoscale Puncturing-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acs.nanolett.0c01979-
dc.identifier.pmid32520569-
dc.identifier.scopuseid_2-s2.0-85088211138-
dc.identifier.volume20-
dc.identifier.issue7-
dc.identifier.spage5473-
dc.identifier.epage5481-
dc.identifier.eissn1530-6992-
dc.identifier.isiWOS:000548893200102-

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