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Article: Development of Fluorescent Probe for CAG-RNA Repeats

TitleDevelopment of Fluorescent Probe for CAG-RNA Repeats
Authors
Issue Date25-Nov-2022
PublisherMDPI
Citation
Biosensors, 2022, v. 12, n. 2 How to Cite?
Abstract

Fluorescent sensing of nucleic acids is a highly sensitive and efficient bioanalytical method for their study in cellular processes, detection and diagnosis in related diseases. However, the design of small molecule fluorescent probes for the selective binding and detection of RNA of a specific sequence is very challenging because of their diverse, dynamic, and flexible structures. By modifying a bis(amidinium)-based small molecular binder that is known to selectively target RNA with CAG repeats using an environment-sensitive fluorophore, a turn-on fluorescent probe featuring aggregation-induced emission (AIE) is successfully developed in this proof-of-concept study. The probe (DB-TPE) exhibits a strong, 19-fold fluorescence enhancement upon binding to a short CAG RNA, and the binding and fluorescence response was found to be specific to the overall RNA secondary structure with A·A mismatches. These promising analytical performances suggest that the probe could be applied in pathological studies, disease progression monitoring, as well as diagnosis of related neurodegenerative diseases due to expanded CAG RNA repeats.


Persistent Identifierhttp://hdl.handle.net/10722/328528
ISSN
2023 Impact Factor: 4.9
2023 SCImago Journal Rankings: 0.707

 

DC FieldValueLanguage
dc.contributor.authorLau, Ho Yan Matthew-
dc.contributor.authorWong, Chun Ho-
dc.contributor.authorChan, Ho Yin Edwin-
dc.contributor.authorAu Yeung, Ho Yu-
dc.date.accessioned2023-06-28T04:45:45Z-
dc.date.available2023-06-28T04:45:45Z-
dc.date.issued2022-11-25-
dc.identifier.citationBiosensors, 2022, v. 12, n. 2-
dc.identifier.issn2079-6374-
dc.identifier.urihttp://hdl.handle.net/10722/328528-
dc.description.abstract<p>Fluorescent sensing of nucleic acids is a highly sensitive and efficient bioanalytical method for their study in cellular processes, detection and diagnosis in related diseases. However, the design of small molecule fluorescent probes for the selective binding and detection of RNA of a specific sequence is very challenging because of their diverse, dynamic, and flexible structures. By modifying a bis(amidinium)-based small molecular binder that is known to selectively target RNA with CAG repeats using an environment-sensitive fluorophore, a turn-on fluorescent probe featuring aggregation-induced emission (AIE) is successfully developed in this proof-of-concept study. The probe (<strong>DB-TPE</strong>) exhibits a strong, 19-fold fluorescence enhancement upon binding to a short CAG RNA, and the binding and fluorescence response was found to be specific to the overall RNA secondary structure with A·A mismatches. These promising analytical performances suggest that the probe could be applied in pathological studies, disease progression monitoring, as well as diagnosis of related neurodegenerative diseases due to expanded CAG RNA repeats.</p>-
dc.languageeng-
dc.publisherMDPI-
dc.relation.ispartofBiosensors-
dc.titleDevelopment of Fluorescent Probe for CAG-RNA Repeats-
dc.typeArticle-
dc.identifier.doi10.3390/bios12121080-
dc.identifier.volume12-
dc.identifier.issue2-
dc.identifier.eissn2079-6374-
dc.identifier.issnl2079-6374-

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