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Article: Activity‐based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells

TitleActivity‐based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells
Authors
Issue Date21-Apr-2023
PublisherChemistry Europe
Citation
Analysis & Sensing, 2023 How to Cite?
Abstract

Development of a fluorescent probe for activity-based sensing of activity of alcohol dehydrogenase, a key enzyme in ethanol biooxidation, is reported. A caged coumarin reporter is released upon the selective oxidation by the enzyme with a strong, >60-fold emission enhancement. The probe has a low cytotoxicity and has been applied in visualising alcohol dehydrogenase activity in HepG2, A549 and HEK293T cells, demonstrating its potential as a convenient, easy-to-use bioanalytical tools in unveiling the roles of the enzyme in alcohol metabolism.


Persistent Identifierhttp://hdl.handle.net/10722/328982
ISSN
2023 Impact Factor: 3.4

 

DC FieldValueLanguage
dc.contributor.authorYau, Wai Yin-
dc.contributor.authorLai, Samuel Kin Man-
dc.contributor.authorBlasco, Pilar-
dc.contributor.authorLi, Xuechen-
dc.contributor.authorNg, Kwan Ming-
dc.contributor.authorLok, Chun Nam-
dc.contributor.authorAu Yeung, Ho Yu-
dc.date.accessioned2023-08-05T07:54:24Z-
dc.date.available2023-08-05T07:54:24Z-
dc.date.issued2023-04-21-
dc.identifier.citationAnalysis & Sensing, 2023-
dc.identifier.issn2629-2742-
dc.identifier.urihttp://hdl.handle.net/10722/328982-
dc.description.abstract<p>Development of a fluorescent probe for activity-based sensing of activity of alcohol dehydrogenase, a key enzyme in ethanol biooxidation, is reported. A caged coumarin reporter is released upon the selective oxidation by the enzyme with a strong, >60-fold emission enhancement. The probe has a low cytotoxicity and has been applied in visualising alcohol dehydrogenase activity in HepG2, A549 and HEK293T cells, demonstrating its potential as a convenient, easy-to-use bioanalytical tools in unveiling the roles of the enzyme in alcohol metabolism.</p>-
dc.languageeng-
dc.publisherChemistry Europe-
dc.relation.ispartofAnalysis & Sensing-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleActivity‐based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells-
dc.typeArticle-
dc.identifier.doi10.1002/anse.202300012-
dc.identifier.eissn2629-2742-
dc.identifier.issnl2629-2742-

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