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- Publisher Website: 10.1016/j.mce.2023.111970
- Scopus: eid_2-s2.0-85160715794
- WOS: WOS:001011611600001
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Article: The involvement of let-7 in hCG-induced progesterone synthesis via regulating p27Kip1 and p21Cip1 expression
Title | The involvement of let-7 in hCG-induced progesterone synthesis via regulating p27Kip1 and p21Cip1 expression |
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Authors | |
Keywords | hCG Let-7 p21Cip1 p27Kip1 Progesterone |
Issue Date | 29-May-2023 |
Publisher | Springer |
Citation | Archives of Gynecology and Obstetrics, 2023, v. 573 How to Cite? |
Abstract | Progesterone is essential in females to maintain a regular menstrual cycle and pregnancy. The luteinizing hormone (LH) surge induces the luteinization of granulosa cells and thecal cells to form the corpus luteum, which is responsible for progesterone synthesis. However, the specific mechanism of how hCG, the analog of LH, regulates progesterone synthesis has yet to be fully discovered. In this study, we found that progesterone level was increased in adult wild-type pregnant mice 2 and 7 days post-coitum, along with a decrease in let-7 expression compared with the estrus stage. Besides, the let-7 expression was negatively correlated with progesterone level in post-delivery day 23 wild-type female mice after being injected with PMSG and hCG. Then, using let-7 transgenic mice and a human granulosa cell line, we found that overexpression of let-7 antagonized progesterone level via targeting p27Kip1 and p21Cip1 and steroidogenic acute regulatory protein (StAR) expression, which is a rate-limiting enzyme in progesterone synthesis. Furthermore, hCG suppressed let-7 expression by stimulating the MAPK pathway. This study elucidated the role of microRNA let-7 in regulating hCG-induced progesterone production and provided new insights into its role in clinical application. |
Persistent Identifier | http://hdl.handle.net/10722/329018 |
ISSN | 2023 Impact Factor: 2.1 2023 SCImago Journal Rankings: 0.782 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chen, J | - |
dc.contributor.author | Liu, K | - |
dc.contributor.author | Liu W | - |
dc.contributor.author | Yeung, WS | - |
dc.date.accessioned | 2023-08-05T07:54:39Z | - |
dc.date.available | 2023-08-05T07:54:39Z | - |
dc.date.issued | 2023-05-29 | - |
dc.identifier.citation | Archives of Gynecology and Obstetrics, 2023, v. 573 | - |
dc.identifier.issn | 0932-0067 | - |
dc.identifier.uri | http://hdl.handle.net/10722/329018 | - |
dc.description.abstract | <p> Progesterone is essential in females to maintain a regular menstrual cycle and pregnancy. The luteinizing hormone (LH) surge induces the luteinization of granulosa cells and thecal cells to form the corpus luteum, which is responsible for progesterone synthesis. However, the specific mechanism of how hCG, the analog of LH, regulates progesterone synthesis has yet to be fully discovered. In this study, we found that progesterone level was increased in adult wild-type pregnant mice 2 and 7 days post-coitum, along with a decrease in let-7 expression compared with the estrus stage. Besides, the let-7 expression was negatively correlated with progesterone level in post-delivery day 23 wild-type female mice after being injected with PMSG and hCG. Then, using let-7 transgenic mice and a human granulosa cell line, we found that overexpression of let-7 antagonized progesterone level via targeting p27<sup>Kip1</sup> and p21<sup>Cip1</sup> and steroidogenic acute regulatory protein (StAR) expression, which is a rate-limiting enzyme in progesterone synthesis. Furthermore, hCG suppressed let-7 expression by stimulating the MAPK pathway. This study elucidated the role of microRNA let-7 in regulating hCG-induced progesterone production and provided new insights into its role in clinical application. <br></p> | - |
dc.language | eng | - |
dc.publisher | Springer | - |
dc.relation.ispartof | Archives of Gynecology and Obstetrics | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | hCG | - |
dc.subject | Let-7 | - |
dc.subject | p21Cip1 | - |
dc.subject | p27Kip1 | - |
dc.subject | Progesterone | - |
dc.title | The involvement of let-7 in hCG-induced progesterone synthesis via regulating p27Kip1 and p21Cip1 expression | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.mce.2023.111970 | - |
dc.identifier.scopus | eid_2-s2.0-85160715794 | - |
dc.identifier.volume | 573 | - |
dc.identifier.eissn | 1432-0711 | - |
dc.identifier.isi | WOS:001011611600001 | - |
dc.identifier.issnl | 0932-0067 | - |