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Article: Expanded Potential Stem Cells from Human Embryos Have an Open Chromatin Configuration with Enhanced Trophoblast Differentiation Ability

TitleExpanded Potential Stem Cells from Human Embryos Have an Open Chromatin Configuration with Enhanced Trophoblast Differentiation Ability
Authors
Keywordschromatin remodeling
expanded potential stem cells
hippo signaling pathway
trophoblast differentiation
Issue Date12-Feb-2023
PublisherWiley Open Access
Citation
Advanced Science, 2023, v. 10, n. 11 How to Cite?
Abstract

Human expanded potential stem cells (hEPSC) have been derived from human embryonic stem cells and induced pluripotent stem cells. Here direct derivation of hEPSC from human pre-implantation embryos is reported. Like the reported hEPSC, the embryo-derived hEPSC (hEPSC-em) exhibit a transcriptome similar to morula, comparable differentiation potency, and high genome editing efficiency. Interestingly, the hEPSC-em show a unique H3 lysine-4 trimethylation (H3K4me3) open chromatin conformation; they possess a higher proportion of H3K4me3 bound broad domain (>5 kb) than the reported hEPSC, naive, and primed embryonic stem cells. The open conformation is associated with enhanced trophoblast differentiation potency with increased trophoblast gene expression upon induction of differentiation and success in derivation of trophoblast stem cells with bona fide characteristics. Hippo signaling is specifically enriched in the H3K4me3 broad domains of the hEPSC-. Knockout of the Hippo signaling gene, YAP1 abolishes the ability of the embryo-derived EPSC to form trophoblast stem cells.


Persistent Identifierhttp://hdl.handle.net/10722/329021
ISSN
2023 Impact Factor: 14.3
2023 SCImago Journal Rankings: 3.914
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, ACH-
dc.contributor.authorLee, YL-
dc.contributor.authorRuan, HZ-
dc.contributor.authorHuang, W-
dc.contributor.authorFong, SW-
dc.contributor.authorTian, SY-
dc.contributor.authorLee, KC-
dc.contributor.authorWu, GM-
dc.contributor.authorTan, YQ-
dc.contributor.authorWong, TCH-
dc.contributor.authorWu, J-
dc.contributor.authorZhang, WY-
dc.contributor.authorCao, DD-
dc.contributor.authorChow, JFC-
dc.contributor.authorLiu, PT-
dc.contributor.authorYeung, WSB-
dc.date.accessioned2023-08-05T07:54:41Z-
dc.date.available2023-08-05T07:54:41Z-
dc.date.issued2023-02-12-
dc.identifier.citationAdvanced Science, 2023, v. 10, n. 11-
dc.identifier.issn2198-3844-
dc.identifier.urihttp://hdl.handle.net/10722/329021-
dc.description.abstract<p> Human expanded potential stem cells (hEPSC) have been derived from human embryonic stem cells and induced pluripotent stem cells. Here direct derivation of hEPSC from human pre-implantation embryos is reported. Like the reported hEPSC, the embryo-derived hEPSC (hEPSC-em) exhibit a transcriptome similar to morula, comparable differentiation potency, and high genome editing efficiency. Interestingly, the hEPSC-em show a unique H3 lysine-4 trimethylation (H3K4me3) open chromatin conformation; they possess a higher proportion of H3K4me3 bound broad domain (>5 kb) than the reported hEPSC, naive, and primed embryonic stem cells. The open conformation is associated with enhanced trophoblast differentiation potency with increased trophoblast gene expression upon induction of differentiation and success in derivation of trophoblast stem cells with bona fide characteristics. Hippo signaling is specifically enriched in the H3K4me3 broad domains of the hEPSC-. Knockout of the Hippo signaling gene, YAP1 abolishes the ability of the embryo-derived EPSC to form trophoblast stem cells. <br></p>-
dc.languageeng-
dc.publisherWiley Open Access-
dc.relation.ispartofAdvanced Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectchromatin remodeling-
dc.subjectexpanded potential stem cells-
dc.subjecthippo signaling pathway-
dc.subjecttrophoblast differentiation-
dc.titleExpanded Potential Stem Cells from Human Embryos Have an Open Chromatin Configuration with Enhanced Trophoblast Differentiation Ability-
dc.typeArticle-
dc.identifier.doi10.1002/advs.202204797-
dc.identifier.scopuseid_2-s2.0-85148034876-
dc.identifier.volume10-
dc.identifier.issue11-
dc.identifier.eissn2198-3844-
dc.identifier.isiWOS:000932759000001-
dc.identifier.issnl2198-3844-

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