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Article: Feasibility of outer membrane protein C as a protein presenting platform targeting antigen to surface of outer membrane vesicle
Title | Feasibility of outer membrane protein C as a protein presenting platform targeting antigen to surface of outer membrane vesicle 外膜蛋白C作为蛋白呈递平台将抗原定位至细菌外膜囊泡表面的可行性 |
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Authors | |
Issue Date | 7-Feb-2022 |
Publisher | 中國生物製品學雜誌編輯部 |
Citation | Chinese Journal of Biologicals, 2022, v. 36, n. 1 How to Cite? |
Abstract | Objective To investigate the feasibility of outer membrane protein C(OmpC)as a protein presenting platform targeting antigen to the surface of outer membrane vesicle(OMV).Methods The recombinant expression plasmid containing ompC gene fragment and Staphylococcus aureus EsxA antigen gene(esxA gene)was constructed,transformed to competent E. coli BL21(DE3),induced by IPTG,and analyzed for expressed product by 12% SDS-PAGE.The total protein of recombinant strain OMV was analyzed by 12% SDS-PAGE,and the localization of fusion protein on the surface of OMV was detected by Western blot and Flow NanoAnalyzer.Results The recombinant expression plasmid containing ompC gene and esxA gene was constructed correctly as proved by sequencing.12% SDS-PAGE showed that the fusion protein OmpC-EsxA had a relative molecular mass of about 57 000,which was consistent with the expected size,while the total protein of OMV showed multiple target protein bands,indicating that recombinant strain OMV was successfully extracted.The fusion protein OmpCEsxA on the surface of recombinant strain OMV specifically bound to mouse antibody against His-Tag,and OMVs labeled with fluorescent antibody were detected by Flow NanoAnalyzer.Conclusion OmpC may be used as a protein presenting platform to locate antigen to OMV surface,which was expected to be applied in the development of antigen presentation vaccine. |
Persistent Identifier | http://hdl.handle.net/10722/329033 |
ISSN | 2023 SCImago Journal Rankings: 0.105 |
DC Field | Value | Language |
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dc.contributor.author | Sun, Jingjing | - |
dc.contributor.author | Huang, Jiandong | - |
dc.date.accessioned | 2023-08-05T07:54:46Z | - |
dc.date.available | 2023-08-05T07:54:46Z | - |
dc.date.issued | 2022-02-07 | - |
dc.identifier.citation | Chinese Journal of Biologicals, 2022, v. 36, n. 1 | - |
dc.identifier.issn | 1004-5503 | - |
dc.identifier.uri | http://hdl.handle.net/10722/329033 | - |
dc.description.abstract | <p>Objective To investigate the feasibility of outer membrane protein C(OmpC)as a protein presenting platform targeting antigen to the surface of outer membrane vesicle(OMV).Methods The recombinant expression plasmid containing ompC gene fragment and Staphylococcus aureus EsxA antigen gene(esxA gene)was constructed,transformed to competent E. coli BL21(DE3),induced by IPTG,and analyzed for expressed product by 12% SDS-PAGE.The total protein of recombinant strain OMV was analyzed by 12% SDS-PAGE,and the localization of fusion protein on the surface of OMV was detected by Western blot and Flow NanoAnalyzer.Results The recombinant expression plasmid containing ompC gene and esxA gene was constructed correctly as proved by sequencing.12% SDS-PAGE showed that the fusion protein OmpC-EsxA had a relative molecular mass of about 57 000,which was consistent with the expected size,while the total protein of OMV showed multiple target protein bands,indicating that recombinant strain OMV was successfully extracted.The fusion protein OmpCEsxA on the surface of recombinant strain OMV specifically bound to mouse antibody against His-Tag,and OMVs labeled with fluorescent antibody were detected by Flow NanoAnalyzer.Conclusion OmpC may be used as a protein presenting platform to locate antigen to OMV surface,which was expected to be applied in the development of antigen presentation vaccine. <br></p> | - |
dc.language | chi | - |
dc.publisher | 中國生物製品學雜誌編輯部 | - |
dc.relation.ispartof | Chinese Journal of Biologicals | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Feasibility of outer membrane protein C as a protein presenting platform targeting antigen to surface of outer membrane vesicle | - |
dc.title | 外膜蛋白C作为蛋白呈递平台将抗原定位至细菌外膜囊泡表面的可行性 | - |
dc.type | Article | - |
dc.identifier.doi | 10.13200/j.cnki.cjb.003788 | - |
dc.identifier.volume | 36 | - |
dc.identifier.issue | 1 | - |
dc.identifier.issnl | 1004-5503 | - |