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Article: Engineered Salmonella inhibits GPX4 expression and induces ferroptosis to suppress glioma growth in vitro and in vivo

TitleEngineered Salmonella inhibits GPX4 expression and induces ferroptosis to suppress glioma growth in vitro and in vivo
Authors
KeywordsEngineered Salmonella
Ferroptosis
Glioma
GPX4
Issue Date23-Jun-2023
PublisherSpringer
Citation
Journal of Neuro-Oncology, 2023 How to Cite?
Abstract

Purpose

Glioma is a life-threatening malignancy where conventional therapies are ineffective. Bacterial cancer therapy has shown potential for glioma treatment, in particular, the facultative anaerobe Salmonella has been extensively studied. Meanwhile, ferroptosis is a newly characterized form of cell death. Nevertheless, the role of ferroptosis in Salmonella-induced tumour cell death remains unclear. Therefore, we aim to elucidate whether Salmonella YB1 exerts therapeutic effects via inducing ferroptosis in glioma.

Methods

Following Salmonella YB1 infection, mRNA sequencing was applied to detect ferroptosis-related gene expression and the levels of reactive oxygen species, malondialdehyde, and glutathione were quantified. Transmission electron microscopy (TEM) was then used to observe the changes in the mitochondrial morphology of glioma cells. The role of ferroptosis in the anti-tumor effect of YB1 was assessed in vivo in mouse tumor xenograft models. 

Results

Whole-transcriptome analysis revealed that Salmonella YB1 infection alters ferroptosis-related gene expression in the U87 glioma cell line. Moreover, we found that Salmonella-induced ferroptosis is correlated with reduced levels of glutathione and glutathione peroxidase-4 (GPX4) and increased levels of reactive oxygen species and malondialdehyde in vitro. Meanwhile, TEM revealed that mitochondria are shrunken and mitochondrial membrane density increases in infected glioma cells. Experiments in vivo further showed that tumor growth in the Salmonella-treated group was significantly slower compared to the control and Fer-1 groups. However, Salmonella-induced tumor suppression can be reversed in vivo by Fer-1 treatment.

Conclusion

Salmonella YB1 inhibits GPX4 expression and induces ferroptosis to suppress glioma growth. Hence, ferroptosis regulation might represent a promising strategy to improve the efficacy of bacterial cancer therapy. 


Persistent Identifierhttp://hdl.handle.net/10722/329058
ISSN
2023 Impact Factor: 3.2
2023 SCImago Journal Rankings: 1.131
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, Jiawen-
dc.contributor.authorLi, Ting-
dc.contributor.authorZhou, Nan-
dc.contributor.authorHe, Yige-
dc.contributor.authorZhong, Jiasheng-
dc.contributor.authorMa, Chengcheng-
dc.contributor.authorZeng, Meiqin-
dc.contributor.authorJi, Jingsen-
dc.contributor.authorHuang, Jian Dong-
dc.contributor.authorKe, Yiquan-
dc.contributor.authorSun, Haitao-
dc.date.accessioned2023-08-05T07:54:57Z-
dc.date.available2023-08-05T07:54:57Z-
dc.date.issued2023-06-23-
dc.identifier.citationJournal of Neuro-Oncology, 2023-
dc.identifier.issn0167-594X-
dc.identifier.urihttp://hdl.handle.net/10722/329058-
dc.description.abstract<p>Purpose <br></p><p>Glioma is a life-threatening malignancy where conventional therapies are ineffective. Bacterial cancer therapy has shown potential for glioma treatment, in particular, the facultative anaerobe Salmonella has been extensively studied. Meanwhile, ferroptosis is a newly characterized form of cell death. Nevertheless, the role of ferroptosis in Salmonella-induced tumour cell death remains unclear. Therefore, we aim to elucidate whether Salmonella YB1 exerts therapeutic effects via inducing ferroptosis in glioma.<br></p><p>Methods <br></p><p>Following Salmonella YB1 infection, mRNA sequencing was applied to detect ferroptosis-related gene expression and the levels of reactive oxygen species, malondialdehyde, and glutathione were quantified. Transmission electron microscopy (TEM) was then used to observe the changes in the mitochondrial morphology of glioma cells. The role of ferroptosis in the anti-tumor effect of YB1 was assessed in vivo in mouse tumor xenograft models. <br></p><p>Results <br></p><p>Whole-transcriptome analysis revealed that Salmonella YB1 infection alters ferroptosis-related gene expression in the U87 glioma cell line. Moreover, we found that Salmonella-induced ferroptosis is correlated with reduced levels of glutathione and glutathione peroxidase-4 (GPX4) and increased levels of reactive oxygen species and malondialdehyde in vitro. Meanwhile, TEM revealed that mitochondria are shrunken and mitochondrial membrane density increases in infected glioma cells. Experiments in vivo further showed that tumor growth in the Salmonella-treated group was significantly slower compared to the control and Fer-1 groups. However, Salmonella-induced tumor suppression can be reversed in vivo by Fer-1 treatment.</p><p>Conclusion <br></p><p>Salmonella YB1 inhibits GPX4 expression and induces ferroptosis to suppress glioma growth. Hence, ferroptosis regulation might represent a promising strategy to improve the efficacy of bacterial cancer therapy. </p>-
dc.languageeng-
dc.publisherSpringer-
dc.relation.ispartofJournal of Neuro-Oncology-
dc.subjectEngineered Salmonella-
dc.subjectFerroptosis-
dc.subjectGlioma-
dc.subjectGPX4-
dc.titleEngineered Salmonella inhibits GPX4 expression and induces ferroptosis to suppress glioma growth in vitro and in vivo-
dc.typeArticle-
dc.identifier.doi10.1007/s11060-023-04369-5-
dc.identifier.scopuseid_2-s2.0-85162653358-
dc.identifier.eissn1573-7373-
dc.identifier.isiWOS:001019480400001-
dc.identifier.issnl0167-594X-

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