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Article: Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis

TitleMultiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis
Authors
KeywordsChromochloris zofingiensis
glycerol-3-phosphate acyltransferase
multiomics
oleaginousness
trait improvement
triacylglycerol
Issue Date2019
Citation
Plant Journal, 2019, v. 98, n. 6, p. 1060-1077 How to Cite?
AbstractChromochloris zofingiensis, featured due to its capability to simultaneously synthesize triacylglycerol (TAG) and astaxanthin, is emerging as a leading candidate alga for production uses. To better understand the oleaginous mechanism of this alga, we conducted a multiomics analysis by systematically integrating time-resolved transcriptomes, lipidomes and metabolomes in response to nitrogen deprivation. The data analysis unraveled the distinct mechanism of TAG accumulation, which involved coordinated stimulation of multiple biological processes including supply of energy and reductants, carbon reallocation from protein and starch, and ‘pushing’ and ‘pulling’ carbon to TAG synthesis. Unlike the model alga Chlamydomonas, de novo fatty acid synthesis in C. zofingiensis was promoted, together with enhanced turnover of both glycolipids and phospholipids, supporting the drastic need of acyls for TAG assembly. Moreover, genomewide analysis identified many key functional enzymes and transcription factors that had engineering potential for TAG modulation. Two genes encoding glycerol-3-phosphate acyltransferase (GPAT), the first committed enzyme for TAG assembly, were found in the C. zofingiensis genome; in vivo functional characterization revealed that extrachloroplastic GPAT instead of chloroplastic GPAT played a central role in TAG synthesis. These findings illuminate distinct oleaginousness mechanisms in C. zofingiensis and pave the way towards rational manipulation of this alga to becone an emerging model for trait improvements.
Persistent Identifierhttp://hdl.handle.net/10722/329555
ISSN
2023 Impact Factor: 6.2
2023 SCImago Journal Rankings: 2.176
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, Jin-
dc.contributor.authorSun, Zheng-
dc.contributor.authorMao, Xuemei-
dc.contributor.authorGerken, Henri-
dc.contributor.authorWang, Xiaofei-
dc.contributor.authorYang, Wenqiang-
dc.date.accessioned2023-08-09T03:33:38Z-
dc.date.available2023-08-09T03:33:38Z-
dc.date.issued2019-
dc.identifier.citationPlant Journal, 2019, v. 98, n. 6, p. 1060-1077-
dc.identifier.issn0960-7412-
dc.identifier.urihttp://hdl.handle.net/10722/329555-
dc.description.abstractChromochloris zofingiensis, featured due to its capability to simultaneously synthesize triacylglycerol (TAG) and astaxanthin, is emerging as a leading candidate alga for production uses. To better understand the oleaginous mechanism of this alga, we conducted a multiomics analysis by systematically integrating time-resolved transcriptomes, lipidomes and metabolomes in response to nitrogen deprivation. The data analysis unraveled the distinct mechanism of TAG accumulation, which involved coordinated stimulation of multiple biological processes including supply of energy and reductants, carbon reallocation from protein and starch, and ‘pushing’ and ‘pulling’ carbon to TAG synthesis. Unlike the model alga Chlamydomonas, de novo fatty acid synthesis in C. zofingiensis was promoted, together with enhanced turnover of both glycolipids and phospholipids, supporting the drastic need of acyls for TAG assembly. Moreover, genomewide analysis identified many key functional enzymes and transcription factors that had engineering potential for TAG modulation. Two genes encoding glycerol-3-phosphate acyltransferase (GPAT), the first committed enzyme for TAG assembly, were found in the C. zofingiensis genome; in vivo functional characterization revealed that extrachloroplastic GPAT instead of chloroplastic GPAT played a central role in TAG synthesis. These findings illuminate distinct oleaginousness mechanisms in C. zofingiensis and pave the way towards rational manipulation of this alga to becone an emerging model for trait improvements.-
dc.languageeng-
dc.relation.ispartofPlant Journal-
dc.subjectChromochloris zofingiensis-
dc.subjectglycerol-3-phosphate acyltransferase-
dc.subjectmultiomics-
dc.subjectoleaginousness-
dc.subjecttrait improvement-
dc.subjecttriacylglycerol-
dc.titleMultiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/tpj.14302-
dc.identifier.pmid30828893-
dc.identifier.scopuseid_2-s2.0-85063666674-
dc.identifier.volume98-
dc.identifier.issue6-
dc.identifier.spage1060-
dc.identifier.epage1077-
dc.identifier.eissn1365-313X-
dc.identifier.isiWOS:000473644100009-

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