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Article: IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries

TitleIPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries
Authors
Keywordsdirected differentiation
human bone marrow stromal cells
remyelination
Schwann cells
sciatic nerve injury
sensory neuron
spinal cord injury
Issue Date25-May-2023
PublisherMDPI
Citation
Cells, 2023, v. 12, n. 11 How to Cite?
Abstract

The in vitro derivation of Schwann cells from human bone marrow stromal cells (hBMSCs) opens avenues for autologous transplantation to achieve remyelination therapy for post-traumatic neural regeneration. Towards this end, we exploited human induced pluripotent stem-cell-derived sensory neurons to direct Schwann-cell-like cells derived from among the hBMSC-neurosphere cells into lineage-committed Schwann cells (hBMSC-dSCs). These cells were seeded into synthetic conduits for bridging critical gaps in a rat model of sciatic nerve injury. With improvement in gait by 12-week post-bridging, evoked signals were also detectable across the bridged nerve. Confocal microscopy revealed axially aligned axons in association with MBP-positive myelin layers across the bridge in contrast to null in non-seeded controls. Myelinating hBMSC-dSCs within the conduit were positive for both MBP and human nucleus marker HuN. We then implanted hBMSC-dSCs into the contused thoracic cord of rats. By 12-week post-implantation, significant improvement in hindlimb motor function was detectable if chondroitinase ABC was co-delivered to the injured site; such cord segments showed axons myelinated by hBMSC-dSCs. Results support translation into a protocol by which lineage-committed hBMSC-dSCs become available for motor function recovery after traumatic injury to both peripheral and central nervous systems.


Persistent Identifierhttp://hdl.handle.net/10722/330934
ISSN
2023 Impact Factor: 5.1
2023 SCImago Journal Rankings: 1.547
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTam, KW-
dc.contributor.authorWong, CY-
dc.contributor.authorWu, KLK-
dc.contributor.authorLam, G-
dc.contributor.authorLiang, XT-
dc.contributor.authorWong, WT-
dc.contributor.authorLi, MTS-
dc.contributor.authorLiu, WY-
dc.contributor.authorCai, S-
dc.contributor.authorShea, GKH-
dc.contributor.authorShum, DKY-
dc.contributor.authorChan, YS-
dc.date.accessioned2023-09-21T06:51:15Z-
dc.date.available2023-09-21T06:51:15Z-
dc.date.issued2023-05-25-
dc.identifier.citationCells, 2023, v. 12, n. 11-
dc.identifier.issn2073-4409-
dc.identifier.urihttp://hdl.handle.net/10722/330934-
dc.description.abstract<p>The in vitro derivation of Schwann cells from human bone marrow stromal cells (hBMSCs) opens avenues for autologous transplantation to achieve remyelination therapy for post-traumatic neural regeneration. Towards this end, we exploited human induced pluripotent stem-cell-derived sensory neurons to direct Schwann-cell-like cells derived from among the hBMSC-neurosphere cells into lineage-committed Schwann cells (hBMSC-dSCs). These cells were seeded into synthetic conduits for bridging critical gaps in a rat model of sciatic nerve injury. With improvement in gait by 12-week post-bridging, evoked signals were also detectable across the bridged nerve. Confocal microscopy revealed axially aligned axons in association with MBP-positive myelin layers across the bridge in contrast to null in non-seeded controls. Myelinating hBMSC-dSCs within the conduit were positive for both MBP and human nucleus marker HuN. We then implanted hBMSC-dSCs into the contused thoracic cord of rats. By 12-week post-implantation, significant improvement in hindlimb motor function was detectable if chondroitinase ABC was co-delivered to the injured site; such cord segments showed axons myelinated by hBMSC-dSCs. Results support translation into a protocol by which lineage-committed hBMSC-dSCs become available for motor function recovery after traumatic injury to both peripheral and central nervous systems.</p>-
dc.languageeng-
dc.publisherMDPI-
dc.relation.ispartofCells-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectdirected differentiation-
dc.subjecthuman bone marrow stromal cells-
dc.subjectremyelination-
dc.subjectSchwann cells-
dc.subjectsciatic nerve injury-
dc.subjectsensory neuron-
dc.subjectspinal cord injury-
dc.titleIPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries-
dc.typeArticle-
dc.identifier.doi10.3390/cells12111479-
dc.identifier.scopuseid_2-s2.0-85161444738-
dc.identifier.volume12-
dc.identifier.issue11-
dc.identifier.eissn2073-4409-
dc.identifier.isiWOS:001006299300001-
dc.identifier.issnl2073-4409-

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