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Article: Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells
Title | Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells |
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Authors | |
Keywords | Cell Biology CRISPR |
Issue Date | 16-Jun-2023 |
Publisher | Cell Press |
Citation | STAR Protocols, 2023, v. 4, n. 2 How to Cite? |
Abstract | We present here a protocol for biallelic tagging of an endogenous gene in human cells using CRISPR-Cas9 editing technology. Using RIF1 as an example, we describe tagging the gene with a mini-auxin-inducible degron and a green fluorescent protein at its C terminus. We detail steps for preparing and designing the sgRNA and homologous repair template, and clone selection and verification. For complete details on the use and execution of this protocol, please refer to Kong et al. |
Persistent Identifier | http://hdl.handle.net/10722/331162 |
ISSN | 2023 Impact Factor: 1.3 2023 SCImago Journal Rankings: 0.955 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Kong, Nannan | - |
dc.contributor.author | Chan, Ying Wai | - |
dc.date.accessioned | 2023-09-21T06:53:17Z | - |
dc.date.available | 2023-09-21T06:53:17Z | - |
dc.date.issued | 2023-06-16 | - |
dc.identifier.citation | STAR Protocols, 2023, v. 4, n. 2 | - |
dc.identifier.issn | 2666-1667 | - |
dc.identifier.uri | http://hdl.handle.net/10722/331162 | - |
dc.description.abstract | <p>We present here a protocol for biallelic tagging of an endogenous gene in human cells using CRISPR-Cas9 editing technology. Using RIF1 as an example, we describe tagging the gene with a mini-auxin-inducible degron and a green fluorescent protein at its C terminus. We detail steps for preparing and designing the sgRNA and homologous repair template, and clone selection and verification. For complete details on the use and execution of this protocol, please refer to Kong et al.<br></p> | - |
dc.language | eng | - |
dc.publisher | Cell Press | - |
dc.relation.ispartof | STAR Protocols | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Cell Biology | - |
dc.subject | CRISPR | - |
dc.title | Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.xpro.2023.102286 | - |
dc.identifier.scopus | eid_2-s2.0-85160267059 | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 2 | - |
dc.identifier.eissn | 2666-1667 | - |
dc.identifier.isi | WOS:001094590600001 | - |
dc.identifier.issnl | 2666-1667 | - |