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Article: Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
Title | Generation of Natural Killer Cells from Human Expanded Potential Stem Cells |
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Authors | |
Issue Date | 13-Jan-2023 |
Publisher | MyJove Corporation |
Citation | Journal of Visualized Experiments, 2023, v. 2023, n. 191 How to Cite? |
Abstract | The differentiation of natural killer (NK) cells from human pluripotent stem cells allows for research on and the manufacture of clinical-grade cellular products for immunotherapy. Described here is a two-phase protocol that uses a serum-free commercial medium and a cocktail of cytokines (interleukin [IL]-3, IL-7, IL-15, stem cell factor [SCF], and FMS-like tyrosine kinase 3 ligand [Ftl3L]) to differentiate human expanded potential stem cells (hEPSCs) into cells that possess NK cell properties in vitro with both 3-dimensional (3D) and 2-dimensional (2D) culture technology. Following this protocol, CD3-CD56+ or CD45+CD56+ NK cells are consistently generated. When cocultured with tumor targets for 3 h, the differentiated products display mild cytotoxicity as compared to an IL-2-independent permanent cell line, NK92mi cells. The protocol preserves the complexity of the differentiation microenvironment by the generation of 3D structures, thus facilitating the study of the spatial relationships between immune cells and their niches. Meanwhile, the 2D culture system enables the routine phenotypical validation of cell differentiation without harming the delicate differentiation niche. |
Persistent Identifier | http://hdl.handle.net/10722/331442 |
ISSN | 2023 Impact Factor: 1.2 2023 SCImago Journal Rankings: 0.449 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Yu, Ching-Poon | - |
dc.contributor.author | Wang, Clement | - |
dc.contributor.author | Hang, Su | - |
dc.contributor.author | Liu, Pentao | - |
dc.contributor.author | Sugimura, Ryohichi | - |
dc.date.accessioned | 2023-09-21T06:55:46Z | - |
dc.date.available | 2023-09-21T06:55:46Z | - |
dc.date.issued | 2023-01-13 | - |
dc.identifier.citation | Journal of Visualized Experiments, 2023, v. 2023, n. 191 | - |
dc.identifier.issn | 1940-087X | - |
dc.identifier.uri | http://hdl.handle.net/10722/331442 | - |
dc.description.abstract | <p>The differentiation of natural killer (NK) cells from human pluripotent stem cells allows for research on and the manufacture of clinical-grade cellular products for immunotherapy. Described here is a two-phase protocol that uses a serum-free commercial medium and a cocktail of cytokines (interleukin [IL]-3, IL-7, IL-15, stem cell factor [SCF], and FMS-like tyrosine kinase 3 ligand [Ftl3L]) to differentiate human expanded potential stem cells (hEPSCs) into cells that possess NK cell properties in vitro with both 3-dimensional (3D) and 2-dimensional (2D) culture technology. Following this protocol, CD3-CD56+ or CD45+CD56+ NK cells are consistently generated. When cocultured with tumor targets for 3 h, the differentiated products display mild cytotoxicity as compared to an IL-2-independent permanent cell line, NK92mi cells. The protocol preserves the complexity of the differentiation microenvironment by the generation of 3D structures, thus facilitating the study of the spatial relationships between immune cells and their niches. Meanwhile, the 2D culture system enables the routine phenotypical validation of cell differentiation without harming the delicate differentiation niche.<br></p> | - |
dc.language | eng | - |
dc.publisher | MyJove Corporation | - |
dc.relation.ispartof | Journal of Visualized Experiments | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Generation of Natural Killer Cells from Human Expanded Potential Stem Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3791/64608 | - |
dc.identifier.scopus | eid_2-s2.0-85146891430 | - |
dc.identifier.volume | 2023 | - |
dc.identifier.issue | 191 | - |
dc.identifier.eissn | 1940-087X | - |
dc.identifier.isi | WOS:000983477000007 | - |
dc.identifier.issnl | 1940-087X | - |