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Article: Multiplexed detection of SARS-CoV-2 based on upconversion luminescence nanoprobe/MXene biosensing platform for COVID-19 point-of-care diagnostics

TitleMultiplexed detection of SARS-CoV-2 based on upconversion luminescence nanoprobe/MXene biosensing platform for COVID-19 point-of-care diagnostics
Authors
KeywordsLanthanide-doped upconversion nanoparticles
Multiplexed detection
Nb2CTx MXene
SARS-CoV-2
Issue Date10-Nov-2022
PublisherElsevier
Citation
Materials & Design, 2022, v. 223 How to Cite?
Abstract

Multiplexed detection is essential in biomedical sciences since it is more efficient and accurate than single-analyte detection. For an accurate early diagnosis of COVID-19, a multiplexed detection strategy is required to avoid false negatives with the existing gold standard assay. Nb2CTx nanosheets were found to efficiently quench the fluorescence emission of lanthanide-doped upconversion luminescence nanoparticles at wavelengths ranging from visible to near-infrared spectrum. Using this broad-spectrum quencher, we developed a label-free FRET-based biosensor for rapid and accurate detection of SARS-CoV-2 RNA. To target ORF and N genes, two types of oligo-modified lanthanide-doped upconversion nanoparticles can be used simultaneously to identify-two sites in one assay via upconversion fluorescence enhancement intensity measurement with detection limits of 15 pM and 914 pM, respectively. Moreover, with multisite cross-validation, this multiplexed and sensitive biosensor is capable of simultaneous and multicolor analysis of two gene fragments of SARS-CoV-2 Omicron variant within minutes in a single homogeneous solution, which significantly improves the detection efficiency. The diagnosis result via our assay is consistent with the PCR result, demonstrating its application in the rapid and accurate screening of multiple genes of SARS-CoV-2 and other infectious diseases.

Keywords: Lanthanide-doped upconversion nanoparticles; Multiplexed detection; Nb2CTx MXene; SARS-CoV-2.


Persistent Identifierhttp://hdl.handle.net/10722/331486
ISSN
2023 Impact Factor: 7.6
2023 SCImago Journal Rankings: 1.684
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSong, Menglin-
dc.contributor.authorMa, Yingjing-
dc.contributor.authorLi, Lihua-
dc.contributor.authorWong, Man-Chung-
dc.contributor.authorWang, Pui-
dc.contributor.authorChen, Jiangkun-
dc.contributor.authorChen, Honglin-
dc.contributor.authorWang, Feng-
dc.contributor.authorHao, Jianhua-
dc.date.accessioned2023-09-21T06:56:15Z-
dc.date.available2023-09-21T06:56:15Z-
dc.date.issued2022-11-10-
dc.identifier.citationMaterials & Design, 2022, v. 223-
dc.identifier.issn0264-1275-
dc.identifier.urihttp://hdl.handle.net/10722/331486-
dc.description.abstract<p>Multiplexed detection is essential in biomedical sciences since it is more efficient and accurate than single-analyte detection. For an accurate early diagnosis of COVID-19, a multiplexed detection strategy is required to avoid false negatives with the existing gold standard assay. Nb<sub>2</sub>CT<sub>x</sub> nanosheets were found to efficiently quench the fluorescence emission of lanthanide-doped upconversion luminescence nanoparticles at wavelengths ranging from visible to near-infrared spectrum. Using this broad-spectrum quencher, we developed a label-free FRET-based biosensor for rapid and accurate detection of SARS-CoV-2 RNA. To target ORF and N genes, two types of oligo-modified lanthanide-doped upconversion nanoparticles can be used simultaneously to identify-two sites in one assay via upconversion fluorescence enhancement intensity measurement with detection limits of 15 pM and 914 pM, respectively. Moreover, with multisite cross-validation, this multiplexed and sensitive biosensor is capable of simultaneous and multicolor analysis of two gene fragments of SARS-CoV-2 Omicron variant within minutes in a single homogeneous solution, which significantly improves the detection efficiency. The diagnosis result <em>via</em> our assay is consistent with the PCR result, demonstrating its application in the rapid and accurate screening of multiple genes of SARS-CoV-2 and other infectious diseases.<br></p><p><strong>Keywords: </strong>Lanthanide-doped upconversion nanoparticles; Multiplexed detection; Nb2CTx MXene; SARS-CoV-2.<br></p>-
dc.languageeng-
dc.publisherElsevier-
dc.relation.ispartofMaterials & Design-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectLanthanide-doped upconversion nanoparticles-
dc.subjectMultiplexed detection-
dc.subjectNb2CTx MXene-
dc.subjectSARS-CoV-2-
dc.titleMultiplexed detection of SARS-CoV-2 based on upconversion luminescence nanoprobe/MXene biosensing platform for COVID-19 point-of-care diagnostics-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.matdes.2022.111249-
dc.identifier.scopuseid_2-s2.0-85140000564-
dc.identifier.volume223-
dc.identifier.eissn1873-4197-
dc.identifier.isiWOS:000897008100008-
dc.identifier.issnl0264-1275-

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