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- Publisher Website: 10.1016/j.phrs.2023.106798
- Scopus: eid_2-s2.0-85159818348
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Article: Exosomes derived from mesenchymal stromal cells promote bone regeneration by delivering miR-182–5p-inhibitor
Title | Exosomes derived from mesenchymal stromal cells promote bone regeneration by delivering miR-182–5p-inhibitor |
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Authors | |
Keywords | BMSC Bone regeneration Exosomes MiRNA Scaffold Tissue engineering |
Issue Date | 1-Jun-2023 |
Publisher | Elsevier |
Citation | Pharmacological Research, 2023, v. 192 How to Cite? |
Abstract | Exosomes, small extracellular vesicles that function as a key regulator of cell-to-cell communication, are emerging as a promising candidate for bone regeneration. Here, we aimed to investigate the effect of exosomes from pre-differentiated human alveolar bone-derived bone marrow mesenchymal stromal cells (AB-BMSCs) carrying specific microRNAs on bone regeneration. Exosomes secreted from AB-BMSCs pre-differentiated for 0 and 7 days were cocultured with BMSCs in vitro to investigate their effect on the differentiation of the BMSCs. MiRNAs from AB-BMSCs at different stages of osteogenic differentiation were analyzed. BMSCs seeded on poly-Llactic acid(PLLA) scaffolds were treated with miRNA antagonist-decorated exosomes to verify their effect on new bone regeneration. Exosomes pre-differentiated for 7 days effectively promoted the differentiation of BMSCs. Bioinformatic analysis revealed that miRNAs within the exosomes were differentially expressed, including the upregulation of osteogenic miRNAs (miR-3182, miR-1468) and downregulation of anti-osteogenic miRNAs (miR182-5p, miR-335-3p, miR-382-5p), causing activation of the PI3K/Akt signaling pathway. The treatment of BMSC-seeded scaffolds with anti-miR-182-5p decorated exosomes demonstrated enhanced osteogenic differentiation and efficient formation of new bone. In conclusion, Osteogenic exosomes secreted from pre-differentiated AB-BMSCs were identified and the gene modification of exosomes provides great potential as a bone regeneration strategy. |
Persistent Identifier | http://hdl.handle.net/10722/331505 |
ISSN | 2023 Impact Factor: 9.1 2023 SCImago Journal Rankings: 2.160 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhu, Qinghai | - |
dc.contributor.author | Tang, Yuting | - |
dc.contributor.author | Zhou, Tian | - |
dc.contributor.author | Yang, Li | - |
dc.contributor.author | Zhang, Gao | - |
dc.contributor.author | Meng, Ying | - |
dc.contributor.author | Zhang, Huixin | - |
dc.contributor.author | Gao, Jun | - |
dc.contributor.author | Wang, Chenxing | - |
dc.contributor.author | Su, Yu-Xiong | - |
dc.contributor.author | Ye, Jinhai | - |
dc.date.accessioned | 2023-09-21T06:56:26Z | - |
dc.date.available | 2023-09-21T06:56:26Z | - |
dc.date.issued | 2023-06-01 | - |
dc.identifier.citation | Pharmacological Research, 2023, v. 192 | - |
dc.identifier.issn | 1043-6618 | - |
dc.identifier.uri | http://hdl.handle.net/10722/331505 | - |
dc.description.abstract | <p>Exosomes, small extracellular vesicles that function as a key regulator of cell-to-cell communication, are emerging as a promising candidate for bone regeneration. Here, we aimed to investigate the effect of exosomes from pre-differentiated human alveolar bone-derived bone marrow mesenchymal stromal cells (AB-BMSCs) carrying specific microRNAs on bone regeneration. Exosomes secreted from AB-BMSCs pre-differentiated for 0 and 7 days were cocultured with BMSCs in vitro to investigate their effect on the differentiation of the BMSCs. MiRNAs from AB-BMSCs at different stages of osteogenic differentiation were analyzed. BMSCs seeded on poly-Llactic acid(PLLA) scaffolds were treated with miRNA antagonist-decorated exosomes to verify their effect on new bone regeneration. Exosomes pre-differentiated for 7 days effectively promoted the differentiation of BMSCs. Bioinformatic analysis revealed that miRNAs within the exosomes were differentially expressed, including the upregulation of osteogenic miRNAs (miR-3182, miR-1468) and downregulation of anti-osteogenic miRNAs (miR182-5p, miR-335-3p, miR-382-5p), causing activation of the PI3K/Akt signaling pathway. The treatment of BMSC-seeded scaffolds with anti-miR-182-5p decorated exosomes demonstrated enhanced osteogenic differentiation and efficient formation of new bone. In conclusion, Osteogenic exosomes secreted from pre-differentiated AB-BMSCs were identified and the gene modification of exosomes provides great potential as a bone regeneration strategy.<br></p> | - |
dc.language | eng | - |
dc.publisher | Elsevier | - |
dc.relation.ispartof | Pharmacological Research | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | BMSC | - |
dc.subject | Bone regeneration | - |
dc.subject | Exosomes | - |
dc.subject | MiRNA | - |
dc.subject | Scaffold | - |
dc.subject | Tissue engineering | - |
dc.title | Exosomes derived from mesenchymal stromal cells promote bone regeneration by delivering miR-182–5p-inhibitor | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.phrs.2023.106798 | - |
dc.identifier.scopus | eid_2-s2.0-85159818348 | - |
dc.identifier.volume | 192 | - |
dc.identifier.eissn | 1096-1186 | - |
dc.identifier.isi | WOS:001008369000001 | - |
dc.identifier.issnl | 1043-6618 | - |