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- Publisher Website: 10.3390/pathogens12080995
- Scopus: eid_2-s2.0-85168892348
- WOS: WOS:001056640600001
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Article: Usefulness of next-generation sequencing in excluding bovine leukemia virus as a cause of adult camel leucosis in dromedaries
Title | Usefulness of next-generation sequencing in excluding bovine leukemia virus as a cause of adult camel leucosis in dromedaries |
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Authors | |
Issue Date | 29-Jul-2023 |
Publisher | MDPI |
Citation | Pathogens, 2023, v. 12, n. 8 How to Cite? |
Abstract | Adult camel leukosis is an emerging hematological and neoplastic disease in dromedaries. It has been hypothesized that bovine leukemia virus (BLV) or its genetic variants may be associated with adult camel leukosis. In this study, we used next-generation sequencing (NGS) to detect all possible viruses in five lung samples from five dromedaries with histopathological evidence of adult camel leukosis and four tissue samples from two control dromedaries. A total throughput of 114.7 Gb was achieved, with an average of 12.7 Gb/sample. For each sample, all the pair-end 151-bp reads were filtered to remove rRNA sequences, bacterial genomes and redundant sequences, resulting in 1-7 Gb clean reads, of which <3% matched to viruses. The largest portion of these viral sequences was composed of bacterial phages. About 100-300 reads in each sample matched "multiple sclerosis-associated retrovirus", but manual analysis showed that they were only repetitive sequences commonly present in mammalian genomes. All viral reads were also extracted for analysis, confirming that no BLV or its genetic variants or any other virus was detected in the nine tissue samples. NGS is not only useful for detecting microorganisms associated with infectious diseases, but also important for excluding an infective cause in scenarios where such a possibility is suspected. |
Persistent Identifier | http://hdl.handle.net/10722/333955 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 0.843 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wernery, U | - |
dc.contributor.author | Teng, JL | - |
dc.contributor.author | Ma, Y | - |
dc.contributor.author | Kinne, J | - |
dc.contributor.author | Yeung, ML | - |
dc.contributor.author | Lau, SK | - |
dc.contributor.author | Woo, PC | - |
dc.date.accessioned | 2023-10-10T03:14:54Z | - |
dc.date.available | 2023-10-10T03:14:54Z | - |
dc.date.issued | 2023-07-29 | - |
dc.identifier.citation | Pathogens, 2023, v. 12, n. 8 | - |
dc.identifier.issn | 2076-0817 | - |
dc.identifier.uri | http://hdl.handle.net/10722/333955 | - |
dc.description.abstract | <p>Adult camel leukosis is an emerging hematological and neoplastic disease in dromedaries. It has been hypothesized that bovine leukemia virus (BLV) or its genetic variants may be associated with adult camel leukosis. In this study, we used next-generation sequencing (NGS) to detect all possible viruses in five lung samples from five dromedaries with histopathological evidence of adult camel leukosis and four tissue samples from two control dromedaries. A total throughput of 114.7 Gb was achieved, with an average of 12.7 Gb/sample. For each sample, all the pair-end 151-bp reads were filtered to remove rRNA sequences, bacterial genomes and redundant sequences, resulting in 1-7 Gb clean reads, of which <3% matched to viruses. The largest portion of these viral sequences was composed of bacterial phages. About 100-300 reads in each sample matched "multiple sclerosis-associated retrovirus", but manual analysis showed that they were only repetitive sequences commonly present in mammalian genomes. All viral reads were also extracted for analysis, confirming that no BLV or its genetic variants or any other virus was detected in the nine tissue samples. NGS is not only useful for detecting microorganisms associated with infectious diseases, but also important for excluding an infective cause in scenarios where such a possibility is suspected.<br></p> | - |
dc.language | eng | - |
dc.publisher | MDPI | - |
dc.relation.ispartof | Pathogens | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Usefulness of next-generation sequencing in excluding bovine leukemia virus as a cause of adult camel leucosis in dromedaries | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/pathogens12080995 | - |
dc.identifier.scopus | eid_2-s2.0-85168892348 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 8 | - |
dc.identifier.eissn | 2076-0817 | - |
dc.identifier.isi | WOS:001056640600001 | - |
dc.identifier.issnl | 2076-0817 | - |