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Article: Selective probing and imaging of cells with single walled carbon nanotubes as near-infrared fluorescent molecules

TitleSelective probing and imaging of cells with single walled carbon nanotubes as near-infrared fluorescent molecules
Authors
Issue Date2008
Citation
Nano Letters, 2008, v. 8, n. 2, p. 586-590 How to Cite?
AbstractFluorescent molecules emitting in the near-infrared (NIR, wavelength ∼0.8-2 μm) are relatively scarce and have been actively sought for biological applications because cells and tissues exhibit little auto-fluorescence in this region. Here, we report the use of semiconducting single-walled carbon nanotubes (SWNTs) as near-infrared fluorescent tags for selective probing of cell surface receptors and cell imaging. Biologically inert SWNTs with polyethyleneglycol functionalization are conjugated to antibodies such as Rituxan to selectively recognize CD20 cell surface receptor on B-cells with little nonspecific binding to negative T-cells and Herceptin to recognize HER2/neu positive breast cancer cells. We image selective SWNT-antibody binding to cells by detecting the intrinsic NIR photoluminescence of nanotubes. We observe ultralow NIR autofluorescence for various cells, an advantageous feature over high autofluorescence and large variations between cells lines in the visible. This establishes SWNTs as novel NIR fluorophors for sensitive and selective biological detections and imaging in vitro and potentially in vivo. Further, our results clearly show that the interactions between carbon nanotubes and living cells are strongly dependent on surface functionalization of nanotubes. © 2008 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/334164
ISSN
2023 Impact Factor: 9.6
2023 SCImago Journal Rankings: 3.411
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWelsher, Kevin-
dc.contributor.authorLiu, Zhuang-
dc.contributor.authorDaranciang, Dan-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-10-20T06:46:11Z-
dc.date.available2023-10-20T06:46:11Z-
dc.date.issued2008-
dc.identifier.citationNano Letters, 2008, v. 8, n. 2, p. 586-590-
dc.identifier.issn1530-6984-
dc.identifier.urihttp://hdl.handle.net/10722/334164-
dc.description.abstractFluorescent molecules emitting in the near-infrared (NIR, wavelength ∼0.8-2 μm) are relatively scarce and have been actively sought for biological applications because cells and tissues exhibit little auto-fluorescence in this region. Here, we report the use of semiconducting single-walled carbon nanotubes (SWNTs) as near-infrared fluorescent tags for selective probing of cell surface receptors and cell imaging. Biologically inert SWNTs with polyethyleneglycol functionalization are conjugated to antibodies such as Rituxan to selectively recognize CD20 cell surface receptor on B-cells with little nonspecific binding to negative T-cells and Herceptin to recognize HER2/neu positive breast cancer cells. We image selective SWNT-antibody binding to cells by detecting the intrinsic NIR photoluminescence of nanotubes. We observe ultralow NIR autofluorescence for various cells, an advantageous feature over high autofluorescence and large variations between cells lines in the visible. This establishes SWNTs as novel NIR fluorophors for sensitive and selective biological detections and imaging in vitro and potentially in vivo. Further, our results clearly show that the interactions between carbon nanotubes and living cells are strongly dependent on surface functionalization of nanotubes. © 2008 American Chemical Society.-
dc.languageeng-
dc.relation.ispartofNano Letters-
dc.titleSelective probing and imaging of cells with single walled carbon nanotubes as near-infrared fluorescent molecules-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/nl072949q-
dc.identifier.pmid18197719-
dc.identifier.scopuseid_2-s2.0-40449138288-
dc.identifier.volume8-
dc.identifier.issue2-
dc.identifier.spage586-
dc.identifier.epage590-
dc.identifier.isiWOS:000253166200039-

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