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Conference Paper: Photoacoustic molecular imaging using single walled carbon nanotubes in living mice

TitlePhotoacoustic molecular imaging using single walled carbon nanotubes in living mice
Authors
Issue Date2009
Citation
Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2009, v. 7177, article no. 717725 How to Cite?
AbstractPhotoacoustic molecular imaging is an emerging technology offering non-invasive high resolution imaging of the molecular expressions of a disease using a photoacoustic imaging agent. Here we demonstrate for the first time the utility of single walled carbon nanotubes (SWNTs) as targeted imaging agents in living mice bearing tumor xenografts. SWNTs were conjugated with polyethylene-glycol-5000 connected to Arg-Gly-Asp (RGD) peptide to target the αvβ3 integrin that is associated with tumor angiogenesis. In-vitro, we characterized the photoacoustic spectra of the particles, their signal linearity and tested their uptake by αvβ3-expressing cells (U87MG). The photoacoustic signal of SWNTs was found not to be affected by the RGD conjugation to the SWNTs and was also found to be highly linear with concentration (R2 = 0.9997 for 25-400nM). The cell uptake studies showed that RGD-targeted SWNTs gave 75% higher photoacoustic signal than non-targeted SWNTs when incubated with U87MG cells. In-vivo, we measured the minimal detectable concentration of SWNTs in living mice by subcutaneously injecting SWNTs at increasing concentrations. The lowest detectable concentration of SWNTs in living mice was found to be 50nM. Finally, we administered RGD-targeted and non-targeted SWNTs via the tail-vein to U87MG tumor-bearing mice (n=4 for each group) and measured the signal from the tumor before and up to 4 hours post-injection. At 4 hours post-injection, tumors of mice injected with RGD-targeted SWNTs showed 8 times higher photoacoustic signal compared with mice injected with non-targeted SWNTs. These results were verified ex-vivo using a Raman microscope that is sensitive to the SWNTs Raman signal. © 2009 SPIE.
Persistent Identifierhttp://hdl.handle.net/10722/334198
ISSN
2023 SCImago Journal Rankings: 0.226
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDe La Zerda, Adam-
dc.contributor.authorZavaleta, Cristina-
dc.contributor.authorKeren, Shay-
dc.contributor.authorVaithilingam, Srikant-
dc.contributor.authorBodapati, Sunil-
dc.contributor.authorTeed, Robert-
dc.contributor.authorLiu, Zhuang-
dc.contributor.authorLevi, Jelena-
dc.contributor.authorSmith, Bryan R.-
dc.contributor.authorMa, Te Jen-
dc.contributor.authorOralkan, Omer-
dc.contributor.authorCheng, Zhen-
dc.contributor.authorChen, Xiaoyuan-
dc.contributor.authorDai, Hongjie-
dc.contributor.authorKhuri-Yakub, Butrus T.-
dc.contributor.authorGambhir, Sanjiv S.-
dc.date.accessioned2023-10-20T06:46:26Z-
dc.date.available2023-10-20T06:46:26Z-
dc.date.issued2009-
dc.identifier.citationProgress in Biomedical Optics and Imaging - Proceedings of SPIE, 2009, v. 7177, article no. 717725-
dc.identifier.issn1605-7422-
dc.identifier.urihttp://hdl.handle.net/10722/334198-
dc.description.abstractPhotoacoustic molecular imaging is an emerging technology offering non-invasive high resolution imaging of the molecular expressions of a disease using a photoacoustic imaging agent. Here we demonstrate for the first time the utility of single walled carbon nanotubes (SWNTs) as targeted imaging agents in living mice bearing tumor xenografts. SWNTs were conjugated with polyethylene-glycol-5000 connected to Arg-Gly-Asp (RGD) peptide to target the αvβ3 integrin that is associated with tumor angiogenesis. In-vitro, we characterized the photoacoustic spectra of the particles, their signal linearity and tested their uptake by αvβ3-expressing cells (U87MG). The photoacoustic signal of SWNTs was found not to be affected by the RGD conjugation to the SWNTs and was also found to be highly linear with concentration (R2 = 0.9997 for 25-400nM). The cell uptake studies showed that RGD-targeted SWNTs gave 75% higher photoacoustic signal than non-targeted SWNTs when incubated with U87MG cells. In-vivo, we measured the minimal detectable concentration of SWNTs in living mice by subcutaneously injecting SWNTs at increasing concentrations. The lowest detectable concentration of SWNTs in living mice was found to be 50nM. Finally, we administered RGD-targeted and non-targeted SWNTs via the tail-vein to U87MG tumor-bearing mice (n=4 for each group) and measured the signal from the tumor before and up to 4 hours post-injection. At 4 hours post-injection, tumors of mice injected with RGD-targeted SWNTs showed 8 times higher photoacoustic signal compared with mice injected with non-targeted SWNTs. These results were verified ex-vivo using a Raman microscope that is sensitive to the SWNTs Raman signal. © 2009 SPIE.-
dc.languageeng-
dc.relation.ispartofProgress in Biomedical Optics and Imaging - Proceedings of SPIE-
dc.titlePhotoacoustic molecular imaging using single walled carbon nanotubes in living mice-
dc.typeConference_Paper-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1117/12.806497-
dc.identifier.scopuseid_2-s2.0-66249139571-
dc.identifier.volume7177-
dc.identifier.spagearticle no. 717725-
dc.identifier.epagearticle no. 717725-
dc.identifier.isiWOS:000285714100066-

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