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Article: Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window

TitleMolecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window
Authors
KeywordsBioconjugate
Clickable dye
Density gradient ultracentrifugation separation
NIR-II molecular imaging
NIR-II multicolor molecular imaging
Issue Date2017
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2017, v. 114, n. 5, p. 962-967 How to Cite?
AbstractFluorescence imaging multiplicity of biological systems is an area of intense focus, currently limited to fluorescence channels in the visible and first near-infrared (NIR-I; ∼700-900 nm) spectral regions. The development of conjugatable fluorophores with longer wavelength emission is highly desired to afford more targeting channels, reduce background autofluorescence, and achieve deeper tissue imaging depths. We have developed NIR-II (1,000-1,700 nm) molecular imaging agents with a bright NIR-II fluorophore through high-efficiency click chemistry to specific molecular antibodies. Relying on buoyant density differences during density gradient ultracentrifugation separations, highly pure NIR-II fluorophore-antibody conjugates emitting ∼1,100 nm were obtained for use as molecular-specific NIR-II probes. This facilitated 3D staining of ∼170-μm histological brain tissues sections on a home-built confocal microscope, demonstrating multicolor molecular imaging across both the NIR-I and NIR-II windows (800-1,700 nm).
Persistent Identifierhttp://hdl.handle.net/10722/334462
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhu, Shoujun-
dc.contributor.authorYang, Qinglai-
dc.contributor.authorAntaris, Alexander L.-
dc.contributor.authorYue, Jingying-
dc.contributor.authorMa, Zhuoran-
dc.contributor.authorWang, Huasen-
dc.contributor.authorHuang, Wei-
dc.contributor.authorWan, Hao-
dc.contributor.authorWang, Joy-
dc.contributor.authorDiao, Shuo-
dc.contributor.authorZhang, Bo-
dc.contributor.authorLi, Xiaoyang-
dc.contributor.authorZhong, Yeteng-
dc.contributor.authorYu, Kuai-
dc.contributor.authorHong, Guosong-
dc.contributor.authorLuo, Jian-
dc.contributor.authorLiang, Yongye-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-10-20T06:48:18Z-
dc.date.available2023-10-20T06:48:18Z-
dc.date.issued2017-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2017, v. 114, n. 5, p. 962-967-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/334462-
dc.description.abstractFluorescence imaging multiplicity of biological systems is an area of intense focus, currently limited to fluorescence channels in the visible and first near-infrared (NIR-I; ∼700-900 nm) spectral regions. The development of conjugatable fluorophores with longer wavelength emission is highly desired to afford more targeting channels, reduce background autofluorescence, and achieve deeper tissue imaging depths. We have developed NIR-II (1,000-1,700 nm) molecular imaging agents with a bright NIR-II fluorophore through high-efficiency click chemistry to specific molecular antibodies. Relying on buoyant density differences during density gradient ultracentrifugation separations, highly pure NIR-II fluorophore-antibody conjugates emitting ∼1,100 nm were obtained for use as molecular-specific NIR-II probes. This facilitated 3D staining of ∼170-μm histological brain tissues sections on a home-built confocal microscope, demonstrating multicolor molecular imaging across both the NIR-I and NIR-II windows (800-1,700 nm).-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.subjectBioconjugate-
dc.subjectClickable dye-
dc.subjectDensity gradient ultracentrifugation separation-
dc.subjectNIR-II molecular imaging-
dc.subjectNIR-II multicolor molecular imaging-
dc.titleMolecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1073/pnas.1617990114-
dc.identifier.pmid28096386-
dc.identifier.scopuseid_2-s2.0-85011295897-
dc.identifier.volume114-
dc.identifier.issue5-
dc.identifier.spage962-
dc.identifier.epage967-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000393196300063-

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