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Article: Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device

TitleMultiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device
Authors
Issue Date2023
Citation
Nature Biomedical Engineering, 2023 How to Cite?
AbstractPortable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.
Persistent Identifierhttp://hdl.handle.net/10722/334990
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, Ying-
dc.contributor.authorYang, Yang-
dc.contributor.authorWang, Guanghui-
dc.contributor.authorWang, Dou-
dc.contributor.authorShao, Pan Lin-
dc.contributor.authorTang, Jiahu-
dc.contributor.authorHe, Tingzhen-
dc.contributor.authorZheng, Jintao-
dc.contributor.authorHu, Ruibin-
dc.contributor.authorLiu, Yiyi-
dc.contributor.authorXu, Ziyi-
dc.contributor.authorNiu, Dan-
dc.contributor.authorLv, Jiahui-
dc.contributor.authorYang, Jingkai-
dc.contributor.authorXiao, Hongjun-
dc.contributor.authorWu, Shuai-
dc.contributor.authorHe, Shuang-
dc.contributor.authorTang, Zhongrong-
dc.contributor.authorLiu, Yan-
dc.contributor.authorTang, Meijie-
dc.contributor.authorJiang, Xingyu-
dc.contributor.authorYuan, Jing-
dc.contributor.authorDai, Hongjie-
dc.contributor.authorZhang, Bo-
dc.date.accessioned2023-10-20T06:52:16Z-
dc.date.available2023-10-20T06:52:16Z-
dc.date.issued2023-
dc.identifier.citationNature Biomedical Engineering, 2023-
dc.identifier.urihttp://hdl.handle.net/10722/334990-
dc.description.abstractPortable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.-
dc.languageeng-
dc.relation.ispartofNature Biomedical Engineering-
dc.titleMultiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/s41551-023-01092-4-
dc.identifier.scopuseid_2-s2.0-85171641892-
dc.identifier.eissn2157-846X-
dc.identifier.isiWOS:001068142600001-

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