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Article: Apoptosis of ovarian cancer cells induced by methylene blue-mediated sonodynamic action

TitleApoptosis of ovarian cancer cells induced by methylene blue-mediated sonodynamic action
Authors
KeywordsApoptosis
Methylene blue
Ovarian cancer
Sonodynamic therapy
Issue Date2011
Citation
Ultrasonics, 2011, v. 51, n. 3, p. 390-395 How to Cite?
AbstractObjective: The present study aims to investigate apoptosis of ovarian cancer cells induced by methylene blue (MB)-mediated sonodynamic therapy (SDT). Methods: The MB concentration was kept constant at 100 μM and ovarian cancer HO-8910 cells were exposed to ultrasound therapy for 5 s with an intensity of 0.46 W/cm2. The cytotoxicity was investigated 24 h after MB-mediated sonodynamic action. Apoptosis was analyzed using a flow cytometer with Annexin V-FITC and propidium iodine (PI) staining as well as fluorescence microscopy with Hoechst 33258 staining. Intracellular reactive oxygen species (ROS) level was measured by flow cytometer with 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) staining. Results: The cytotoxicity of MB-mediated SDT on HO-8910 cells after MB-mediated SDT was significantly higher than those of other treatments including ultrasound alone, MB alone and sham treatment. Flow cytometric analysis showed a significant increase in the early and late apoptotic cell populations by MB-mediated SDT of HO-8910 cells. Nuclear condensation and increased ROS levels were also found in HO-8910 cells treated by MB-mediated SDT. Conclusions: Our findings demonstrated that MB-mediated sonodynamic action significantly induced apoptosis of HO-8910 cells and an increase in intracellular ROS level. This indicates that apoptosis is an important mechanism of cell death induced by MB-mediated SDT. Thus, MB-mediated SDT might be a potential therapeutic strategy for combating ovarian cancer. © 2010 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/335748
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 1.093
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXiang, Junyan-
dc.contributor.authorXia, Xinshu-
dc.contributor.authorJiang, Yuan-
dc.contributor.authorLeung, Albert Wingnang-
dc.contributor.authorWang, Xinna-
dc.contributor.authorXu, Jing-
dc.contributor.authorWang, Ping-
dc.contributor.authorYu, Heping-
dc.contributor.authorBai, Dingqun-
dc.contributor.authorXu, Chuanshan-
dc.date.accessioned2023-12-28T08:48:28Z-
dc.date.available2023-12-28T08:48:28Z-
dc.date.issued2011-
dc.identifier.citationUltrasonics, 2011, v. 51, n. 3, p. 390-395-
dc.identifier.issn0041-624X-
dc.identifier.urihttp://hdl.handle.net/10722/335748-
dc.description.abstractObjective: The present study aims to investigate apoptosis of ovarian cancer cells induced by methylene blue (MB)-mediated sonodynamic therapy (SDT). Methods: The MB concentration was kept constant at 100 μM and ovarian cancer HO-8910 cells were exposed to ultrasound therapy for 5 s with an intensity of 0.46 W/cm2. The cytotoxicity was investigated 24 h after MB-mediated sonodynamic action. Apoptosis was analyzed using a flow cytometer with Annexin V-FITC and propidium iodine (PI) staining as well as fluorescence microscopy with Hoechst 33258 staining. Intracellular reactive oxygen species (ROS) level was measured by flow cytometer with 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) staining. Results: The cytotoxicity of MB-mediated SDT on HO-8910 cells after MB-mediated SDT was significantly higher than those of other treatments including ultrasound alone, MB alone and sham treatment. Flow cytometric analysis showed a significant increase in the early and late apoptotic cell populations by MB-mediated SDT of HO-8910 cells. Nuclear condensation and increased ROS levels were also found in HO-8910 cells treated by MB-mediated SDT. Conclusions: Our findings demonstrated that MB-mediated sonodynamic action significantly induced apoptosis of HO-8910 cells and an increase in intracellular ROS level. This indicates that apoptosis is an important mechanism of cell death induced by MB-mediated SDT. Thus, MB-mediated SDT might be a potential therapeutic strategy for combating ovarian cancer. © 2010 Elsevier B.V. All rights reserved.-
dc.languageeng-
dc.relation.ispartofUltrasonics-
dc.subjectApoptosis-
dc.subjectMethylene blue-
dc.subjectOvarian cancer-
dc.subjectSonodynamic therapy-
dc.titleApoptosis of ovarian cancer cells induced by methylene blue-mediated sonodynamic action-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ultras.2010.11.005-
dc.identifier.pmid21147492-
dc.identifier.scopuseid_2-s2.0-78650938479-
dc.identifier.volume51-
dc.identifier.issue3-
dc.identifier.spage390-
dc.identifier.epage395-
dc.identifier.isiWOS:000285919400019-

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