File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The ion channel activity of the SARS-coronavirus 3a protein is linked to its pro-apoptotic function

TitleThe ion channel activity of the SARS-coronavirus 3a protein is linked to its pro-apoptotic function
Authors
KeywordsDrosophila
Ion channel
Severe acute respiratory syndrome
Site-directed mutagenesis
Vero E6
Issue Date2009
Citation
International Journal of Biochemistry and Cell Biology, 2009, v. 41, n. 11, p. 2232-2239 How to Cite?
AbstractThe severe acute respiratory syndrome-coronavirus (SARS-CoV) caused an outbreak of atypical pneumonia in 2003. The SARS-CoV viral genome encodes several proteins which have no homology to proteins in any other coronaviruses, and a number of these proteins have been implicated in viral cytopathies. One such protein is 3a, which is also known as X1, ORF3 and U274. 3a expression is detected in both SARS-CoV infected cultured cells and patients. Among the different functions identified, 3a is a capable of inducing apoptosis. We previously showed that caspase pathways are involved in 3a-induced apoptosis. In this study, we attempted to find out protein domains on 3a that are essential for its pro-apoptotic function. Protein sequence analysis reveals that 3a possesses three major protein signatures, the cysteine-rich, Yxxφ{symbol} and diacidic domains. We showed that 3a proteins carrying respective mutations in these protein domains exhibit reduced pro-apoptotic activities, indicating the importance of these domains on 3a's pro-apoptotic function. It was previously reported that 3a possesses potassium ion channel activity. We further demonstrated that the blockade of 3a's potassium channel activity abolished caspase-dependent apoptosis. This report provides the first evidence that ion channel activity of 3a is required for its pro-apoptotic function. As ion channel activity has been reported to regulate apoptosis in different pathologic conditions, finding ways to modulate the ion channel activity may offer a new direction toward the inhibition of apoptosis triggered by SARS-CoV. © 2009 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/336076
ISSN
2023 Impact Factor: 3.4
2023 SCImago Journal Rankings: 1.079
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, Chak Ming-
dc.contributor.authorTsoi, Ho-
dc.contributor.authorChan, Wing Man-
dc.contributor.authorZhai, Shenyu-
dc.contributor.authorWong, Ching On-
dc.contributor.authorYao, Xiaoqiang-
dc.contributor.authorChan, Wood Yee-
dc.contributor.authorTsui, Stephen Kwok Wing-
dc.contributor.authorChan, Ho Yin Edwin-
dc.date.accessioned2024-01-15T08:23:13Z-
dc.date.available2024-01-15T08:23:13Z-
dc.date.issued2009-
dc.identifier.citationInternational Journal of Biochemistry and Cell Biology, 2009, v. 41, n. 11, p. 2232-2239-
dc.identifier.issn1357-2725-
dc.identifier.urihttp://hdl.handle.net/10722/336076-
dc.description.abstractThe severe acute respiratory syndrome-coronavirus (SARS-CoV) caused an outbreak of atypical pneumonia in 2003. The SARS-CoV viral genome encodes several proteins which have no homology to proteins in any other coronaviruses, and a number of these proteins have been implicated in viral cytopathies. One such protein is 3a, which is also known as X1, ORF3 and U274. 3a expression is detected in both SARS-CoV infected cultured cells and patients. Among the different functions identified, 3a is a capable of inducing apoptosis. We previously showed that caspase pathways are involved in 3a-induced apoptosis. In this study, we attempted to find out protein domains on 3a that are essential for its pro-apoptotic function. Protein sequence analysis reveals that 3a possesses three major protein signatures, the cysteine-rich, Yxxφ{symbol} and diacidic domains. We showed that 3a proteins carrying respective mutations in these protein domains exhibit reduced pro-apoptotic activities, indicating the importance of these domains on 3a's pro-apoptotic function. It was previously reported that 3a possesses potassium ion channel activity. We further demonstrated that the blockade of 3a's potassium channel activity abolished caspase-dependent apoptosis. This report provides the first evidence that ion channel activity of 3a is required for its pro-apoptotic function. As ion channel activity has been reported to regulate apoptosis in different pathologic conditions, finding ways to modulate the ion channel activity may offer a new direction toward the inhibition of apoptosis triggered by SARS-CoV. © 2009 Elsevier Ltd.-
dc.languageeng-
dc.relation.ispartofInternational Journal of Biochemistry and Cell Biology-
dc.subjectDrosophila-
dc.subjectIon channel-
dc.subjectSevere acute respiratory syndrome-
dc.subjectSite-directed mutagenesis-
dc.subjectVero E6-
dc.titleThe ion channel activity of the SARS-coronavirus 3a protein is linked to its pro-apoptotic function-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biocel.2009.04.019-
dc.identifier.pmid19398035-
dc.identifier.scopuseid_2-s2.0-70349290577-
dc.identifier.volume41-
dc.identifier.issue11-
dc.identifier.spage2232-
dc.identifier.epage2239-
dc.identifier.isiWOS:000271124700019-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats