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- Publisher Website: 10.3390/polym13050704
- Scopus: eid_2-s2.0-85102269453
- WOS: WOS:000628410300001
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Article: Reinforced universal adhesive by ribose crosslinker: A novel strategy in adhesive dentistry
Title | Reinforced universal adhesive by ribose crosslinker: A novel strategy in adhesive dentistry |
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Authors | |
Keywords | Crosslinking Dentin Hybrid layer Ribose Universal adhesives |
Issue Date | 2021 |
Citation | Polymers, 2021, v. 13, n. 5, p. 1-16 How to Cite? |
Abstract | Enzymatic biodegradation of demineralized collagen fibrils could lead to the reduction of resin–dentin bond strength. Therefore, methods that provide protection to collagen fibrils appear to be a pragmatic solution to improve bond strength. Thus, the study’s aim was to investigate the effect of ribose (RB) on demineralized resin–dentin specimens in a modified universal adhesive. Dentin specimens were obtained, standardized and then bonded in vitro with a commercial multi-mode adhesive modified with 0, 0.5%, 1%, and 2% RB, restored with resin composite, and tested for micro-tensile bond strength (µTBS) after storage for 24 h in artificial saliva. Scanning electron microscopy (SEM) was performed to analyze resin–dentin interface. Contact angles were analyzed using a contact angle analyzer. Depth of penetration of adhesives and nanoleakage were assessed using micro-Raman spectroscopy and silver tracing. Molecular docking studies were carried out using Schrodinger small-molecule drug discovery suite 2019-4. Matrix metalloproteinases-2 (MMP-2) and cathepsin-K activities in RB-treated specimens were quantified using enzyme-linked immunosorbent assay (ELISA). The significance level was set at α = 0.05 for all statistical analyses. Incorporation of RB at 1% or 2% is of significant potential (p < 0.05) as it can be associated with improved wettability on dentin surfaces (0.5% had the lowest contact angle) as well as appreciable hybrid layer quality, and higher resin penetration. Improvement of the adhesive bond strength was shown when adding RB at 1% concentration to universal adhesive (p < 0.05). Modified adhesive increased the resistance of collagen degradation by inhibiting MMP-2 and cathepsin-K. A higher RB concentration was associated with improved results (p < 0.01). D-ribose showed favorable negative binding to collagen. In conclusion, universal adhesive using 1% or 2% RB helped in maintaining dentin collagen scaffold and proved to be successful in improving wettability, protease inhibition, and stability of demineralized dentin substrates. A more favorable substrate is created which, in turn, leads to a more stable dentin-adhesive bond. This could lead to more advantageous outcomes in a clinical scenario where a stable bond may result in longevity of the dental restoration. |
Persistent Identifier | http://hdl.handle.net/10722/336271 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Bourgi, Rim | - |
dc.contributor.author | Daood, Umer | - |
dc.contributor.author | Bijle, Mohammed Nadeem | - |
dc.contributor.author | Fawzy, Amr | - |
dc.contributor.author | Ghaleb, Maroun | - |
dc.contributor.author | Hardan, Louis | - |
dc.date.accessioned | 2024-01-15T08:25:04Z | - |
dc.date.available | 2024-01-15T08:25:04Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Polymers, 2021, v. 13, n. 5, p. 1-16 | - |
dc.identifier.uri | http://hdl.handle.net/10722/336271 | - |
dc.description.abstract | Enzymatic biodegradation of demineralized collagen fibrils could lead to the reduction of resin–dentin bond strength. Therefore, methods that provide protection to collagen fibrils appear to be a pragmatic solution to improve bond strength. Thus, the study’s aim was to investigate the effect of ribose (RB) on demineralized resin–dentin specimens in a modified universal adhesive. Dentin specimens were obtained, standardized and then bonded in vitro with a commercial multi-mode adhesive modified with 0, 0.5%, 1%, and 2% RB, restored with resin composite, and tested for micro-tensile bond strength (µTBS) after storage for 24 h in artificial saliva. Scanning electron microscopy (SEM) was performed to analyze resin–dentin interface. Contact angles were analyzed using a contact angle analyzer. Depth of penetration of adhesives and nanoleakage were assessed using micro-Raman spectroscopy and silver tracing. Molecular docking studies were carried out using Schrodinger small-molecule drug discovery suite 2019-4. Matrix metalloproteinases-2 (MMP-2) and cathepsin-K activities in RB-treated specimens were quantified using enzyme-linked immunosorbent assay (ELISA). The significance level was set at α = 0.05 for all statistical analyses. Incorporation of RB at 1% or 2% is of significant potential (p < 0.05) as it can be associated with improved wettability on dentin surfaces (0.5% had the lowest contact angle) as well as appreciable hybrid layer quality, and higher resin penetration. Improvement of the adhesive bond strength was shown when adding RB at 1% concentration to universal adhesive (p < 0.05). Modified adhesive increased the resistance of collagen degradation by inhibiting MMP-2 and cathepsin-K. A higher RB concentration was associated with improved results (p < 0.01). D-ribose showed favorable negative binding to collagen. In conclusion, universal adhesive using 1% or 2% RB helped in maintaining dentin collagen scaffold and proved to be successful in improving wettability, protease inhibition, and stability of demineralized dentin substrates. A more favorable substrate is created which, in turn, leads to a more stable dentin-adhesive bond. This could lead to more advantageous outcomes in a clinical scenario where a stable bond may result in longevity of the dental restoration. | - |
dc.language | eng | - |
dc.relation.ispartof | Polymers | - |
dc.subject | Crosslinking | - |
dc.subject | Dentin | - |
dc.subject | Hybrid layer | - |
dc.subject | Ribose | - |
dc.subject | Universal adhesives | - |
dc.title | Reinforced universal adhesive by ribose crosslinker: A novel strategy in adhesive dentistry | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3390/polym13050704 | - |
dc.identifier.scopus | eid_2-s2.0-85102269453 | - |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 16 | - |
dc.identifier.eissn | 2073-4360 | - |
dc.identifier.isi | WOS:000628410300001 | - |