File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3390/molecules26216605
- Scopus: eid_2-s2.0-85118399844
- PMID: 34771014
- WOS: WOS:000718661400001
Supplementary
- Citations:
- Appears in Collections:
Article: Concentration-dependent multi-potentiality of l-arginine: Antimicrobial effect, hydroxyapatite stability, and mmps inhibition
Title | Concentration-dependent multi-potentiality of l-arginine: Antimicrobial effect, hydroxyapatite stability, and mmps inhibition |
---|---|
Authors | |
Keywords | Arginine Biofilm Crystals Matrix metalloproteinase Raman spectroscopy |
Issue Date | 2021 |
Citation | Molecules, 2021, v. 26, n. 21, article no. 6605 How to Cite? |
Abstract | This study’s objective was to examine L-arginine (L-arg) supplementation’s effect on mono-species biofilm (Streptococcus mutans/Streptococcus sanguinis) growth and underlying enamel substrates. The experimental groups were 1%, 2%, and 4% arg, and 0.9% NaCl was used as the vehicle control. Sterilised enamel blocks were subjected to 7-day treatment with test solutions and S. mutans/S. sanguinis inoculum in BHI. Post-treatment, the treated biofilms stained for live/dead bacterial cells were analysed using confocal microscopy. The enamel specimens were analysed using X-ray diffraction crystallography (XRD), Raman spectroscopy (RS), and transmission electron microscopy (TEM). The molecular interactions between arg and MMP-2/MMP-9 were determined by computational molecular docking and MMP assays. With increasing arg concentrations, bacterial survival significantly decreased (p < 0.05). The XRD peak intensity with 1%/2% arg was significantly higher than with 4% arg and the control (p < 0.05). The bands associated with the mineral phase by RS were significantly accentuated in the 1%/2% arg specimens compared to in other groups (p < 0.05). The TEM analysis revealed that 4% arg exhibited an ill-defined shape of enamel crystals. Docking of arg molecules to MMPs appears feasible, with arg inhibiting MMP-2/MMP-9 (p < 0.05). L-arginine supplementation has an antimicrobial effect on mono-species biofilm. L-arginine treatment at lower (1%/2%) concentrations exhibits enamel hydroxyapatite stability, while the molecule has the potential to inhibit MMP-2/MMP-9. |
Persistent Identifier | http://hdl.handle.net/10722/336290 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bijle, Mohammed Nadeem | - |
dc.contributor.author | Pichika, Mallikarjuna Rao | - |
dc.contributor.author | Mak, Kit Kay | - |
dc.contributor.author | Parolia, Abhishek | - |
dc.contributor.author | Babar, Muneer Gohar | - |
dc.contributor.author | Yiu, Cynthia | - |
dc.contributor.author | Daood, Umer | - |
dc.date.accessioned | 2024-01-15T08:25:15Z | - |
dc.date.available | 2024-01-15T08:25:15Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Molecules, 2021, v. 26, n. 21, article no. 6605 | - |
dc.identifier.uri | http://hdl.handle.net/10722/336290 | - |
dc.description.abstract | This study’s objective was to examine L-arginine (L-arg) supplementation’s effect on mono-species biofilm (Streptococcus mutans/Streptococcus sanguinis) growth and underlying enamel substrates. The experimental groups were 1%, 2%, and 4% arg, and 0.9% NaCl was used as the vehicle control. Sterilised enamel blocks were subjected to 7-day treatment with test solutions and S. mutans/S. sanguinis inoculum in BHI. Post-treatment, the treated biofilms stained for live/dead bacterial cells were analysed using confocal microscopy. The enamel specimens were analysed using X-ray diffraction crystallography (XRD), Raman spectroscopy (RS), and transmission electron microscopy (TEM). The molecular interactions between arg and MMP-2/MMP-9 were determined by computational molecular docking and MMP assays. With increasing arg concentrations, bacterial survival significantly decreased (p < 0.05). The XRD peak intensity with 1%/2% arg was significantly higher than with 4% arg and the control (p < 0.05). The bands associated with the mineral phase by RS were significantly accentuated in the 1%/2% arg specimens compared to in other groups (p < 0.05). The TEM analysis revealed that 4% arg exhibited an ill-defined shape of enamel crystals. Docking of arg molecules to MMPs appears feasible, with arg inhibiting MMP-2/MMP-9 (p < 0.05). L-arginine supplementation has an antimicrobial effect on mono-species biofilm. L-arginine treatment at lower (1%/2%) concentrations exhibits enamel hydroxyapatite stability, while the molecule has the potential to inhibit MMP-2/MMP-9. | - |
dc.language | eng | - |
dc.relation.ispartof | Molecules | - |
dc.subject | Arginine | - |
dc.subject | Biofilm | - |
dc.subject | Crystals | - |
dc.subject | Matrix metalloproteinase | - |
dc.subject | Raman spectroscopy | - |
dc.title | Concentration-dependent multi-potentiality of l-arginine: Antimicrobial effect, hydroxyapatite stability, and mmps inhibition | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3390/molecules26216605 | - |
dc.identifier.pmid | 34771014 | - |
dc.identifier.scopus | eid_2-s2.0-85118399844 | - |
dc.identifier.volume | 26 | - |
dc.identifier.issue | 21 | - |
dc.identifier.spage | article no. 6605 | - |
dc.identifier.epage | article no. 6605 | - |
dc.identifier.eissn | 1420-3049 | - |
dc.identifier.isi | WOS:000718661400001 | - |